. "Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes" . "Pro kondenzaci chromosom\u016F b\u011Bhem meotick\u00E9ho zr\u00E1n\u00ED prase\u010D\u00EDch oocyt\u016F nen\u00ED nezbytn\u00E1 ani aktivita Aurory B ani fosforylace histonu H3"@cs . . . . "[E303B2FECF6A]" . . . "Kubelka, Michal" . "8"^^ . "74" . "Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes" . "Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes"@en . "488100" . "2"^^ . "P(GA204/03/0816), Z(AV0Z50450515)" . . . "Neither Aurora B Activity nor Histone H3 Phosphorylation is Essential for Chromosome Condensation During Meiotic Maturation of Porcine Oocytes"@en . . . "905;912" . "2"^^ . . . "Aurora B kin\u00E1za je tzv. chromosom\u00E1ln\u00ED \u201Epassenger\u201C protein, kter\u00FD je nezbytn\u00FD pro \u0159adu proces\u016F prob\u00EDhaj\u00EDch\u00EDch b\u011Bhem mit\u00F3zy. Jeho aktivita je regulov\u00E1na spojen\u00EDm s dal\u0161\u00EDmi \u201Epassenger\u201C proteiny, INCENP a Survivin, a jeho fosforylac\u00ED na Thr-232. V t\u00E9to pr\u00E1ci, byla studov\u00E1na exprese a fosforylace (Thr-232) Aurory B kin\u00E1zy b\u011Bhem meiotick\u00E9ho zr\u00E1n\u00ED prase\u010D\u00EDch oocyt\u016F spolu s fosforylac\u00ED histonu H3 a v korelaci s kondenzac\u00ED chromosom\u016F. Uk\u00E1zalo se, \u017Ee fosforylace histonu H3 na Ser-10, ale ne na Ser-28, koreluje s postupnou kondenzac\u00ED chromosom\u016F b\u011Bhem zr\u00E1n\u00ED oocyt\u016F; fosforylace na Ser 10 za\u010D\u00EDn\u00E1 v okol\u00ED doby rozpadu jadern\u00E9 membr\u00E1ny, s maxim\u00E1ln\u00ED aktivitou v metaf\u00E1zi I, zat\u00EDmco fosforylace na Ser-28 se b\u011Bhem zr\u00E1n\u00ED oocyt\u016F v\u00FDrazn\u011B nem\u011Bn\u00ED. Po o\u0161et\u0159en\u00ED oocyt\u016F 50 mM Butyrolactonem I (BL-I), inhibitor cyklin dependentn\u00EDch kin\u00E1z, \u010Di cykloheximidem ( 10mg/ml) \u2013 inhibitor proteosynt\u00E9zy, doch\u00E1z\u00ED k zastaven\u00ED oocytu ve f\u00E1zi tzv. z\u00E1rode\u010Dn\u00E9ho v\u00E1\u010Dku (germinal vesicle \u2013 GV), ve kter\u00E9 z\u016Fst\u00E1v\u00E1 neporu\u0161ena jadern\u00E1 membr\u00E1na, nicm..."@cs . "Aurora-B kinase is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora B kinase during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 mM Butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10mg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germin..." . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Biology of Reproduction" . "Aurora-B kinase is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora B kinase during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser-10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 mM Butyrolactone I (BL-I), an inhibitor of cyclin-dependent kinases, or cycloheximide (10mg/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germin..."@en . . "gamete biology; kinases; meiosis"@en . "Pro kondenzaci chromosom\u016F b\u011Bhem meotick\u00E9ho zr\u00E1n\u00ED prase\u010D\u00EDch oocyt\u016F nen\u00ED nezbytn\u00E1 ani aktivita Aurory B ani fosforylace histonu H3"@cs . "Jel\u00EDnkov\u00E1, Lucie" . "RIV/67985904:_____/06:00040831" . . . "5" . "0006-3363" . . "RIV/67985904:_____/06:00040831!RIV07-AV0-67985904" . .