"PKB/AKT is involved in resumption of meiosis in mouse oocytes" . "Baran, V." . "PKB/AKT is involved in resumption of meiosis in mouse oocytes"@en . "Motl\u00EDk, Jan" . . "6"^^ . "P(GA523/03/0857), Z(AV0Z50450515)" . "Schultz, R. M." . "492188" . "FR - Francouzsk\u00E1 republika" . "Activation of PKB (protein kinase B) and CDK1 (cyclin-dependent kinase 1) during maturation of mouse oocytes was analysed. PKB phosphorylation and activation preceded GVBD (germinal vesicle breakdown) in oocytes maturing either in vitro or in vivo. Activation was transient and PKB activity was markedly reduced when virtually all of the oocytes had undergone GVBD. Treatment of oocytes with butyrolactone I, a specific inhibitor of CDK1, revealed that PKB activation is independent of CDK1 activity. LY-294002, an inhibitor of phosphoinositide 3-kinase\u2013PKB signalling, suppressed activation of PKB and CDK1 as well as resumption of meiosis. During resumption of meiosis, PKB phosphorylated on Ser473 is associated with nuclear membrane and centrosome, whereas PKB phosphorylated on Thr308 is localized on centrosome only. The presence of phosphorylated PKB on centrosome at the time of GVBD suggests its important role for an initial CDK1 activation."@en . . "Byla provedena anal\u00FDza aktivace PKB (proteinkin\u00E1za B) a CDK1 (cyklin-dependentn\u00ED kin\u00E1za 1) v pr\u016Fb\u011Bhu zr\u00E1n\u00ED oocyt\u016F my\u0161i. B\u011Bhem zr\u00E1n\u00ED oocyt\u016F v podm\u00EDnk\u00E1ch in vitro a in vivo fosforylace a aktivace PKB p\u0159edch\u00E1zela GVBD (rozpad z\u00E1rode\u010Dn\u00E9ho v\u00E1\u010Dku). Tato aktivace byla do\u010Dasn\u00E1 a aktivita PKB v\u00FDrazn\u011B poklesla po t\u00E9, co t\u00E9m\u011B\u0159 v\u0161echny oocyty pro\u0161ly GVBD. O\u0161et\u0159en\u00ED oocyt\u016F butyrolactonem I (inhibitor CDK1) prok\u00E1zalo, \u017Ee k aktivaci PKB doch\u00E1z\u00ED nez\u00E1visle na aktivit\u011B CDK1. LY-294002, inhibitor fosfoinositid 3-kin\u00E1zov\u00E9-PKB sign\u00E1ln\u00ED dr\u00E1hy, potla\u010Dil aktivaci PKB a CDK1 jako\u017E i znovuzah\u00E1jen\u00ED mei\u00F3zy. B\u011Bhem znovuzah\u00E1jen\u00ED mei\u00F3zy PKB fosforylovan\u00E1 na Ser473 byla lokalizov\u00E1na na jadern\u00E9 membr\u00E1n\u011B a na centroz\u00F3mu, zat\u00EDmco PKB fosforylovan\u00E1 na Thr308 byla detekov\u00E1na pouze na centroz\u00F3mu. V\u00FDskyt fosforylovan\u00E9 PKB na centroz\u00F3mu v pr\u016Fb\u011Bhu GVBD nazna\u010Duje, \u017Ee PKB m\u00E1 v\u00FDznamnou funkci p\u0159i po\u010D\u00E1te\u010Dn\u00ED aktivaci CDK1."@cs . . . . "RIV/67985904:_____/06:00038826" . . . "Biology of the Cell" . . "13"^^ . . "0248-4900" . "4"^^ . "PKB/AKT se pod\u00EDl\u00ED na znovuzah\u00E1jen\u00ED mei\u00F3zy oocyt\u016F my\u0161i"@cs . "98" . . . "CDK1; germinal vesicle breakdown; okadaic acid"@en . "Kubelka, Michal" . "\u0160olc, Petr" . . . . "PKB/AKT is involved in resumption of meiosis in mouse oocytes" . "Kalous, Jaroslav" . "PKB/AKT se pod\u00EDl\u00ED na znovuzah\u00E1jen\u00ED mei\u00F3zy oocyt\u016F my\u0161i"@cs . . . . "[29DD534C2566]" . "-" . "PKB/AKT is involved in resumption of meiosis in mouse oocytes"@en . . . "111;123" . "RIV/67985904:_____/06:00038826!RIV06-AV0-67985904" . . "Activation of PKB (protein kinase B) and CDK1 (cyclin-dependent kinase 1) during maturation of mouse oocytes was analysed. PKB phosphorylation and activation preceded GVBD (germinal vesicle breakdown) in oocytes maturing either in vitro or in vivo. Activation was transient and PKB activity was markedly reduced when virtually all of the oocytes had undergone GVBD. Treatment of oocytes with butyrolactone I, a specific inhibitor of CDK1, revealed that PKB activation is independent of CDK1 activity. LY-294002, an inhibitor of phosphoinositide 3-kinase\u2013PKB signalling, suppressed activation of PKB and CDK1 as well as resumption of meiosis. During resumption of meiosis, PKB phosphorylated on Ser473 is associated with nuclear membrane and centrosome, whereas PKB phosphorylated on Thr308 is localized on centrosome only. The presence of phosphorylated PKB on centrosome at the time of GVBD suggests its important role for an initial CDK1 activation." .