"[391D023C2840]" . . "37;47" . "Stojkovic, M." . "Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle."@en . "0"^^ . "1" . "0"^^ . "1"^^ . "Motl\u00EDk, Jan" . "M-phase synchronized bovine blastomeres were used to study the effect of nuclear-cytoplasmic synchronization on the developmental potential after nuclear transfer (NT). The capacity of nocodazole and benomyl to reversibly synchronize blastomeres from embryos in M-phase was evaluated. Nocodazole reversibly arrested bovine embryos at the studied stages and induced high rates of M-phases in morulae and compact morulae. In contrast, benomyl was less efficient than nocodazole to synchronize in M-phase. After transfer of an M-phase blastomere, premature chromatin condensation was the prevalent finding 1 hr post-fusion (hpf). Condensed chromosomes non-arranged in the equatorial plate (1-3 hpf) that acquired an organized structure over time (3-7 hpf) were subsequently observed. Anaphase;telophase structures were predominantly recorded at 4-9 hpf. About 50% of the embryos activated at both 3-4 and 6-7 hpf extruded a polar body-like structure 5 hr after activation, but this was not observed in embryos activated"@en . . "Alberio, R." . . . "Wolf, E." . . "11"^^ . "Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle." . "Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle." . "Molecular Reproduction and Development" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "5"^^ . "705493" . "57" . . "RIV/67985904:_____/00:21003030!RIV/2003/AV0/A21003/N" . . "P(KSK2038602), Z(AV0Z5045916)" . . . "Behavior of M-phase synchronized blastomeres after nuclear transfer in cattle."@en . "M-phase synchronized bovine blastomeres were used to study the effect of nuclear-cytoplasmic synchronization on the developmental potential after nuclear transfer (NT). The capacity of nocodazole and benomyl to reversibly synchronize blastomeres from embryos in M-phase was evaluated. Nocodazole reversibly arrested bovine embryos at the studied stages and induced high rates of M-phases in morulae and compact morulae. In contrast, benomyl was less efficient than nocodazole to synchronize in M-phase. After transfer of an M-phase blastomere, premature chromatin condensation was the prevalent finding 1 hr post-fusion (hpf). Condensed chromosomes non-arranged in the equatorial plate (1-3 hpf) that acquired an organized structure over time (3-7 hpf) were subsequently observed. Anaphase;telophase structures were predominantly recorded at 4-9 hpf. About 50% of the embryos activated at both 3-4 and 6-7 hpf extruded a polar body-like structure 5 hr after activation, but this was not observed in embryos activated" . . "RIV/67985904:_____/00:21003030" . "1040-452X" . . "N/A"@en . . . . "Zakhartchenko, V." .