. "N/A"@en . "Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases."@en . "Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases." . "703878" . "P(KSK2038602), Z(AV0Z5045916)" . "Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohemine, either alone or in combination with ionomycin, is able to activate young matured bovine oocytes. Furthermore, the effect of bohemine on the patterns of DNA synthesis after pronuclear formation as well as changes in his tone H1 kinase and MAP kinase activities during the process of activation were studied. Our results with bohemine show that the specific inhibition of CDKs in metaphase II bovine oocytes induces parthenogenetic activation in a dose-dependent manner (25, 50, and 100 mu M, respectively), either alone (3%, 30%, and 50%) or in combination with ionomycin (30%, 70%, and 87.5%). A single pronucleus and extrusion of the second polar body was observed (97%) when Ca2+ influx was"@en . . "4" . . "Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases."@en . "RIV/67985904:_____/00:21003029!RIV/2003/AV0/A21003/N" . "Kubelka, Michal" . . . "55" . . "Motl\u00EDk, Jan" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "1040-452X" . "422;432" . "2"^^ . . "Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohemine, either alone or in combination with ionomycin, is able to activate young matured bovine oocytes. Furthermore, the effect of bohemine on the patterns of DNA synthesis after pronuclear formation as well as changes in his tone H1 kinase and MAP kinase activities during the process of activation were studied. Our results with bohemine show that the specific inhibition of CDKs in metaphase II bovine oocytes induces parthenogenetic activation in a dose-dependent manner (25, 50, and 100 mu M, respectively), either alone (3%, 30%, and 50%) or in combination with ionomycin (30%, 70%, and 87.5%). A single pronucleus and extrusion of the second polar body was observed (97%) when Ca2+ influx was" . "[E0D2AAE60364]" . "Alberio, R." . "0"^^ . "0"^^ . "Molecular Reproduction and Development" . . "11"^^ . "6"^^ . . . "Activation of bovine oocytes by specific inhibition of cyclin-dependent kinases." . "Hajduch, M." . . "RIV/67985904:_____/00:21003029" . "Zakhartchenko, V." . . . "Wolf, E." . .