. "Vodi\u010Dka, Petr" . "RIV/67985904:_____/00:21003012!RIV/2003/AV0/A21003/N" . "64" . "1;111" . "P(GV524/96/K162), P(KSK2038602), Z(AV0Z5045916)" . "Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence." . "Bovine oocytes originated from follicles of two different size categories (medium (M), 3-6mm and small (S), 1-2mm) were cultured for 24 and 70h, respectively, in a meiosis-inhibiting medium (MIM) supplemented with 100&mgr;M butyrolactone I (BL I). At the end of culture, cumulus oocyte complexes (COC) from M and S follicles were labeled by 3H-uridine for 30min. The autoradiography (ARG) of semi-thin sections of COC showed the labeling of the germinal vesicles (GV) as: (1) The COC of the M category labeled immediately after isolation from follicles showed only weak labeling (+) of the GV. The COC of the S category labeled immediately after their isolation showed mostly intensive labeling (++++, +++) of the GV. (2) When the COC were labeled after 24 and 70h of culture in MIM, no labeling was observed in the M category. The S category of oocytes showed the slightly decreased labeling (+++, ++) after 24h and negligible labeling after 70h of culture. The pattern of very intensive labeling of granulosa cell" . . . "[88E3B913D664]" . "Animal Reproduction Science" . . "N/A"@en . . . "707810" . . . . "Pavlok, Anton\u00EDn" . "Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence."@en . "NL - Nizozemsko" . "Ka\u0148ka, Ji\u0159\u00ED" . . "1-2" . "Bovine oocytes originated from follicles of two different size categories (medium (M), 3-6mm and small (S), 1-2mm) were cultured for 24 and 70h, respectively, in a meiosis-inhibiting medium (MIM) supplemented with 100&mgr;M butyrolactone I (BL I). At the end of culture, cumulus oocyte complexes (COC) from M and S follicles were labeled by 3H-uridine for 30min. The autoradiography (ARG) of semi-thin sections of COC showed the labeling of the germinal vesicles (GV) as: (1) The COC of the M category labeled immediately after isolation from follicles showed only weak labeling (+) of the GV. The COC of the S category labeled immediately after their isolation showed mostly intensive labeling (++++, +++) of the GV. (2) When the COC were labeled after 24 and 70h of culture in MIM, no labeling was observed in the M category. The S category of oocytes showed the slightly decreased labeling (+++, ++) after 24h and negligible labeling after 70h of culture. The pattern of very intensive labeling of granulosa cell"@en . . "0"^^ . "4"^^ . . "0"^^ . "4"^^ . "Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence." . . "RIV/67985904:_____/00:21003012" . . . "Motl\u00EDk, Jan" . . "0378-4320" . . "11"^^ . . . "Culture of bovine oocytes from small antral follicles in meiosis-inhibiting medium with butyrolactone I: RNA synthesis, nucleolar morphology and meiotic competence."@en .