"Biology of Reproduction" . "In this study, butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinases, was shown to block germinal vesicle (CV) breakdown (GVBD) in bovine oocytes in a concentration-dependent manner; GVBD was almost totally inhibited over the course of 24-48 h of culture when 100 mu M BL I was included in tissue culture medium 199 containing either polyvinyl alcohol or BSA. Correlated with this inhibition was the failure of either p34(cdc2) kinase or mitogen-activated protein (MAP) kinase to become activated, and it was unlikely that BL I directly inhibited MAP kinase, since 100 mu M BL I did not inhibit MAP kinase activity present in extracts obtained from metaphase II-arrested bovine eggs that possess high levels of MAP kinase activity. Nevertheless, the formation of highly condensed bivalents was observed in 78% of the BL I-treated GV-intact oocytes, This result suggests that chromosome condensation during first meiosis in bovine oocytes does not require the activity of either p34cdc2" . "706002" . . . "292;302" . . . . "RIV/67985904:_____/00:21003004!RIV/2003/AV0/A21003/N" . . "11"^^ . . "Kubelka, Michal" . "P(GV524/96/K162), P(KSK2038602), Z(AV0Z5045916)" . . . "Butyrolactone I Reversibly Inhibits Meiotic Maturation of Bovine Oocytes, Without Influencing Chromosome Condensation Activity."@en . . . "0006-3363" . "N/A"@en . . "Butyrolactone I Reversibly Inhibits Meiotic Maturation of Bovine Oocytes, Without Influencing Chromosome Condensation Activity." . . "Motl\u00EDk, Jan" . . "3"^^ . "0"^^ . "Butyrolactone I Reversibly Inhibits Meiotic Maturation of Bovine Oocytes, Without Influencing Chromosome Condensation Activity."@en . "0"^^ . "62" . . "In this study, butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinases, was shown to block germinal vesicle (CV) breakdown (GVBD) in bovine oocytes in a concentration-dependent manner; GVBD was almost totally inhibited over the course of 24-48 h of culture when 100 mu M BL I was included in tissue culture medium 199 containing either polyvinyl alcohol or BSA. Correlated with this inhibition was the failure of either p34(cdc2) kinase or mitogen-activated protein (MAP) kinase to become activated, and it was unlikely that BL I directly inhibited MAP kinase, since 100 mu M BL I did not inhibit MAP kinase activity present in extracts obtained from metaphase II-arrested bovine eggs that possess high levels of MAP kinase activity. Nevertheless, the formation of highly condensed bivalents was observed in 78% of the BL I-treated GV-intact oocytes, This result suggests that chromosome condensation during first meiosis in bovine oocytes does not require the activity of either p34cdc2"@en . "[083B513F35CE]" . "Schultz, R. M." . "2"^^ . "N/A" . "RIV/67985904:_____/00:21003004" . . . "Butyrolactone I Reversibly Inhibits Meiotic Maturation of Bovine Oocytes, Without Influencing Chromosome Condensation Activity." . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" .