. . "Pimkov\u00E1, K." . "000298749300041" . "Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes"@en . . "172555" . "Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes" . . . "1618-2642" . "surface plasmon resonance; myelodysplastic syndromes; vascular endothelial growth factor"@en . "7"^^ . "Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes"@en . . "10.1007/s00216-011-5395-3" . . "P(KAN200670701), P(NS10633), Z(AV0Z20670512), Z(MZ0UHKT2005)" . . "Hegnerov\u00E1, Kate\u0159ina" . "Homola, Ji\u0159\u00ED" . "7"^^ . "Analytical and Bioanalytical Chemistry" . "\u010Cerm\u00E1k, J." . . . "[27DE8AC5DA9E]" . . "3"^^ . . . "The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use."@en . "Dyr, J. E." . . "402" . "1" . . "Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes" . "Suttnar, J." . . . . "The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use." . "Bockov\u00E1, Mark\u00E9ta" . "RIV/67985882:_____/12:00374555!RIV13-AV0-67985882" . . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "RIV/67985882:_____/12:00374555" . .