. . "Optimalizovan\u00FD protokol pro transgenezi hlodavc\u016F pomoc\u00ED traspozonov\u00FDch vektor\u016F Sleeping Beauty"@cs . "94411" . . "RIV/67985823:_____/13:00427117" . "Optimalizovan\u00FD protokol pro transgenezi hlodavc\u016F pomoc\u00ED traspozonov\u00FDch vektor\u016F Sleeping Beauty" . . . . . "4"^^ . . . . "Z\u00EDdek, V\u00E1clav" . "Uhl\u00EDk, L." . "D\u00EDl\u010D\u00ED v\u00FDsledek, kter\u00FD umo\u017En\u00ED efektivn\u00ED transgenezi pro produkci transgenn\u00EDch potkan\u016F pro biomedic\u00EDnsk\u00FD v\u00FDzkum" . "I, P(TA02010013)" . "Protokol je zalo\u017Een\u00FD na injekci prvojader oplozen\u00FDch oocyt\u016F sm\u011Bs\u00ED syntetick\u00E9 mRNA, k\u00F3duj\u00EDc\u00ED SB100X hyperaktivn\u00ED transpoz\u00E1zu spole\u010Dn\u011B s cirkul\u00E1rn\u00ED plasmidovou DNA nesouc\u00ED transgenn\u00ED konstrukt, kter\u00FD m\u00E1 po stran\u00E1ch vazebn\u00E1 m\u00EDsta pro transpoz\u00E1zu. Po translaci transpoz\u00E1zov\u00E9 mRNA dojde k enzymatick\u00E9 excizi transgenn\u00ED kazety z plazmidov\u00E9 DNA a n\u00E1sledn\u00E9 permanentn\u00ED inzerci do genomu. Vysoce efektivn\u00ED transgeneze pomoc\u00ED tohoto protokolu trv\u00E1 p\u0159ibli\u017En\u011B 3 m\u011Bs\u00EDce"@cs . "3"^^ . "Landa, Vladim\u00EDr" . "Protokol SB transgeneze" . "Optimalizovan\u00FD protokol pro transgenezi hlodavc\u016F pomoc\u00ED traspozonov\u00FDch vektor\u016F Sleeping Beauty"@cs . "Protokol je zalo\u017Een\u00FD na injekci prvojader oplozen\u00FDch oocyt\u016F sm\u011Bs\u00ED syntetick\u00E9 mRNA, k\u00F3duj\u00EDc\u00ED SB100X hyperaktivn\u00ED transpoz\u00E1zu spole\u010Dn\u011B s cirkul\u00E1rn\u00ED plasmidovou DNA nesouc\u00ED transgenn\u00ED konstrukt, kter\u00FD m\u00E1 po stran\u00E1ch vazebn\u00E1 m\u00EDsta pro transpoz\u00E1zu. Po translaci transpoz\u00E1zov\u00E9 mRNA dojde k enzymatick\u00E9 excizi transgenn\u00ED kazety z plazmidov\u00E9 DNA a n\u00E1sledn\u00E9 permanentn\u00ED inzerci do genomu. Vysoce efektivn\u00ED transgeneze pomoc\u00ED tohoto protokolu trv\u00E1 p\u0159ibli\u017En\u011B 3 m\u011Bs\u00EDce" . . . "Pravenec, Michal" . "Optimalizovan\u00FD protokol pro transgenezi hlodavc\u016F pomoc\u00ED traspozonov\u00FDch vektor\u016F Sleeping Beauty" . . "transposon transgenesis; Sleeping Beauty; rat"@en . . "Optimized protocol for rodent transgenesis with the Sleeping Beauty transposon vectors"@en . "The procedure is based on co-injection of synthetic mRNA encoding the SB100X hyperactive transposase, together with circular plasmid DNA carrying a transgene construct flanked by binding sites for the transposase, into the pronuclei of fertilized oocytes. Upon translation of the transposase mRNA, enzyme-mediated excision of the transgene cassettes from the injected plasmids followed by permanent genomic insertion. Highly effective transgenesis by using this protocol takes approximately 3 months"@en . "RIV/67985823:_____/13:00427117!RIV14-TA0-67985823" . . . . . . "Optimized protocol for rodent transgenesis with the Sleeping Beauty transposon vectors"@en . "[2AA7D94986C1]" . "Technick\u00E9 parametry v\u00FDsledku jsou detailn\u011B popsan\u00E9 v \u010Dl\u00E1nku Ivics, Landa, Z\u00EDdek, Pravenec et al. Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons. Nature Protocols 9: 773-793, 2014" . . . . .