. . . "Herynkov\u00E1, Pavla" . "3"^^ . . "3"^^ . "[918532ABC140]" . "Fyziologick\u00FD \u00FAstav AV\u010CR, v.v" . "S. cerevisiae BYT1245 a BYT12345" . "316175" . . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations" . "S. cerevisiae mutant strains lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA5, TOK1) and potassium importers (TRK1, TRK2) have been constructed. The TRK1 gene has been substituted by the LEU2 gene; the three ENA genes, TOK1 and TRK2 genes have been replaced by the kanMX cassette. Finally, the LEU2 and kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background lacking either 6 or 7 transporters have been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryots"@en . . "P(GA204/08/0354), P(IAA500110801), P(LC531), Z(AV0Z50110509)" . . "Petrezs\u00E9lyov\u00E1, Silvia" . . "Sychrov\u00E1, Hana" . . . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations" . . "S. cerevisiae mutant strains lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA5, TOK1) and potassium importers (TRK1, TRK2) have been constructed. The TRK1 gene has been substituted by the LEU2 gene; the three ENA genes, TOK1 and TRK2 genes have been replaced by the kanMX cassette. Finally, the LEU2 and kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background lacking either 6 or 7 transporters have been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryots" . "RIV/67985823:_____/09:00337845" . . . . . "n\u00E1klady v 300 000,- K\u010D, licen\u010Dn\u00ED poplatek 50 000,- K\u010D" . . . . "RIV/67985823:_____/09:00337845!RIV10-MSM-67985823" . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations"@en . . . . "yeast heterologous expression; S. cerevisiae; salt-sensitive mutant"@en . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations"@en . . . "Mutantn\u00ED kmeny S. cerevisiae BYT1245 (nha1 ena1-5 trk1 trk2) a BYT12345 (nha1 ena1-5 tok1 trk1 trk2) s vysokou citlivost\u00ED k sol\u00EDm alkalick\u00FDch kation\u016F a z\u00E1rove\u0148 s neschopnost\u00ED r\u016Fst na m\u00E9di\u00EDch s limitn\u011B n\u00EDzk\u00FDm obsahem draseln\u00FDch sol\u00ED" . . .