"340936" . "RIV/67985823:_____/09:00337821!RIV10-MSM-67985823" . "2"^^ . "P(LC531), Z(AV0Z50110509)" . . "Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata" . "Krauke, Yannick" . "2"^^ . "Fyziologick\u00FD \u00FAstav AV\u010CR, v.v" . "Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata" . . "n\u00E1klady 300 000,- K\u010D, licen\u010Dn\u00ED poplatek 50 000,- K\u010D" . . . "Sychrov\u00E1, Hana" . "The ENA1 gene encoding plasma membrane Na+ATPase in the pathogenic C. glabrata was amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Ena1 proteins tagged with GFP at their C termini). Resulting plasmids allow the heterologous expression of Candida glabrata Ena1 transporter in S. cerevisiae strains and the study of its substrate specificity, activity and/or localization"@en . . . . "RIV/67985823:_____/09:00337821" . . . "The ENA1 gene encoding plasma membrane Na+ATPase in the pathogenic C. glabrata was amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Ena1 proteins tagged with GFP at their C termini). Resulting plasmids allow the heterologous expression of Candida glabrata Ena1 transporter in S. cerevisiae strains and the study of its substrate specificity, activity and/or localization" . . . . . . . . "CandidaENA1-YEP352/pGRU1" . "Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata"@en . . . . . "Steriln\u00ED vodn\u00E9 roztoky dvou plasmidov\u00FDch DNA ur\u010Den\u00FDch pro transformaci E.coli (selekce AmpR) nebo S. cerevisiae (selekce Ura+) a po\u010D\u00EDta\u010Dov\u00E9 soubory zn\u00E1zor\u0148uj\u00EDc\u00ED sekvence DNA plasmid\u016F" . . "Candida glabrata; plasma-membrane Na+ATPase; yeast heterologous expression"@en . . "Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata"@en . "[8639097B742D]" .