. . "369137" . . . "Mutantn\u00ED kmen S. cerevisiae ABK1 (nha1 ena1-4 nhx1 kha1) s vysokou citlivost\u00ED k sol\u00EDm alkalick\u00FDch kation\u016F, vhodn\u00FD pro heterologn\u00ED expresi vnitrobun\u011B\u010Dn\u00FDch transport\u00E9r\u016F drasl\u00EDku a sod\u00EDku z jin\u00FDch eukaryot" . "P(IAA5011407), P(LC531), Z(AV0Z50110509)" . . . "Genetically modified S. cerevisiae strain lacking transporters of alkali metal cations" . "Genov\u011B modifikovan\u00FD kmen S. cerevisiae postr\u00E1daj\u00EDc\u00ED transportn\u00ED syst\u00E9my pro kationy alkalick\u00FDch kov\u016F"@cs . . "S. cerevisiae ABK1" . "Genov\u011B modifikovan\u00FD kmen S. cerevisiae postr\u00E1daj\u00EDc\u00ED transportn\u00ED syst\u00E9my pro kationy alkalick\u00FDch kov\u016F"@cs . "Genetically modified S. cerevisiae strain lacking transporters of alkali metal cations" . . . "FGU-C" . . . . "Genetically modified S. cerevisiae strain lacking transporters of alkali metal cations"@en . "An S. cerevisiae mutant strain lacking the genes encoding alkali-metal-cation transporters (NHA1, ENA1, ENA2, ENA3, ENA4, KHA1, NHX1) has been constructed from a strain lacking only the plasma membrane cation exporters (Ena1-4 and Nha1). The NHX1 gene has been substituted by the TRP1 gene, the KHA1 gene has been replaced by the loxP sequence. A unique GMO strain in the W303 background has been created to study heterologously expressed intracellular sodium and potassium transporters from both lower and higher eukaryots" . . "2"^^ . . . . . . "yeast heterologous expression; S. cerevisiae; salt-sensitive mutant"@en . "An S. cerevisiae mutant strain lacking the genes encoding alkali-metal-cation transporters (NHA1, ENA1, ENA2, ENA3, ENA4, KHA1, NHX1) has been constructed from a strain lacking only the plasma membrane cation exporters (Ena1-4 and Nha1). The NHX1 gene has been substituted by the TRP1 gene, the KHA1 gene has been replaced by the loxP sequence. A unique GMO strain in the W303 background has been created to study heterologously expressed intracellular sodium and potassium transporters from both lower and higher eukaryots"@en . "Genetically modified S. cerevisiae strain lacking transporters of alkali metal cations"@en . . "Mare\u0161ov\u00E1, Lydie" . "2"^^ . . "Byl vytvo\u0159en mutantn\u00ED kmen S. cerevisiae postr\u00E1daj\u00EDc\u00ED geny k\u00F3duj\u00EDc\u00ED transportn\u00ED syst\u00E9my pro kationy alkalick\u00FDch kov\u016F (NHA1, ENA1, ENA2, ENA3, ENA4, KHA1, NHX1) z kmene postr\u00E1daj\u00EDc\u00EDho pouze exportn\u00ED syst\u00E9my kationt\u016F v plasmatick\u00E9 membr\u00E1n\u011B (Ena1-4, Nha1). Gen NHX1 byl nahrazen genem TRP1 a gen KHA1 byl vym\u011Bn\u011Bn za sekvenci loxP. Vznikl tak unik\u00E1tn\u00ED GMO v genetick\u00E9m pozad\u00ED W303 s vyu\u017Eit\u00EDm pro studium heterologn\u011B exprimovan\u00FDch vnitrobun\u011B\u010Dn\u00FDch transport\u00E9r\u016F sodn\u00FDch a draseln\u00FDch kation\u016F z ni\u017E\u0161\u00EDch i vy\u0161\u0161\u00EDch eukaryot"@cs . . "RIV/67985823:_____/08:00324283!RIV09-AV0-67985823" . "[42A0D7696D09]" . "Sychrov\u00E1, Hana" . "n\u00E1klady 500 000 K\u010D, licen\u010Dn\u00ED poplatek 50 000 K\u010D" . . . "RIV/67985823:_____/08:00324283" .