. "RIV/67985823:_____/08:00324265" . . . "CandidaCNH1-YEP352/pGRU1" . . "394529" . "Kinclov\u00E1, Olga" . "[4BA820090187]" . . "Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)" . "RIV/67985823:_____/08:00324265!RIV09-AV0-67985823" . "The CNH1 genes encoding homologous plasma membrane Na+/H+ antiporters from pathogenic C. albicans, C. glabrata, C. parapsilosis or C. dubliniensis were amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Cnh1 proteins tagged with GFP at their C termini). Resulting 8 plasmids allow the heterologous expression of Candida sp. Cnh1 antiporters in S. cerevisiae strains and the study and comparison of their substrate specificity, activity or localization" . "Sychrov\u00E1, Hana" . . . "Candida sp.; plasma-membrane Na+/H+ antiporter; heterologous expression"@en . . "FGU-C" . . . . . "Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)" . . . . . . "Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)"@en . "Geny CNH1 k\u00F3duj\u00EDc\u00ED homologn\u00ED Na+/H+-antiportn\u00ED syst\u00E9my plasmatick\u00E9 membr\u00E1ny z patogenn\u00EDch kvasinek C. albicans, C. glabrata, C. parapsilosis nebo C. dubliniensis byly amplifikov\u00E1ny PCR a klonov\u00E1ny za konstitutivn\u00ED promotor genu NHA1 ze Saccharomyces cerevisiae do \u010Dlunkov\u00FDch vektor\u016F S. cerevisiae/E.coli (URA3/AmpR) YEp352 nebo pGRU1 (pro expresi protein\u016F Cnh1 zna\u010Den\u00FDch GFP na C konci). V\u00FDsledn\u00FDch 8 plasmid\u016F umo\u017E\u0148uje heterologn\u00ED expresi antiporter\u016F Cnh1 z Candida sp. v kmenech S. cerevisiae a t\u00EDm i studium a porovn\u00E1n\u00ED jejich substratov\u00E9 specifity, transportn\u00ED aktivity nebo lokalizace v bu\u0148k\u00E1ch"@cs . "n\u00E1klady 300 000,- K\u010D, licen\u010Dn\u00ED poplatek 50 000,- K\u010D" . . . "The CNH1 genes encoding homologous plasma membrane Na+/H+ antiporters from pathogenic C. albicans, C. glabrata, C. parapsilosis or C. dubliniensis were amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Cnh1 proteins tagged with GFP at their C termini). Resulting 8 plasmids allow the heterologous expression of Candida sp. Cnh1 antiporters in S. cerevisiae strains and the study and comparison of their substrate specificity, activity or localization"@en . . "Krauke, Yannick" . . "3"^^ . "Sada mnohokopiov\u00FDch plasmid\u016F pro studium transportn\u00EDch vlastnost\u00ED a lokalizace Na+/H+-antiportn\u00EDch syst\u00E9m\u016F ze 4 patogenn\u00EDch druh\u016F kvasinek rodu Candida (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)"@cs . . "P(KJB5011307), P(LC531), Z(AV0Z50110509)" . "Steriln\u00ED vodn\u00E9 roztoky osmi plasmidov\u00FDch DNA ur\u010Den\u00FDch pro transformaci E.coli (selekce AmpR) nebo S. cerevisiae (selekce Ura+) a t\u0159i po\u010D\u00EDta\u010Dov\u00E9 soubory zn\u00E1zor\u0148uj\u00EDc\u00ED sekvence DNA plasmid\u016F" . "3"^^ . "Sada mnohokopiov\u00FDch plasmid\u016F pro studium transportn\u00EDch vlastnost\u00ED a lokalizace Na+/H+-antiportn\u00EDch syst\u00E9m\u016F ze 4 patogenn\u00EDch druh\u016F kvasinek rodu Candida (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)"@cs . "Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)"@en . . .