. . . . . . "Teisinger, Jan" . . "2"^^ . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou mutac\u00ED Cdelta42 zkracuj\u00EDc\u00ED d\u00E9lku karboxylov\u00E9ho konce, zvy\u0161uj\u00EDc\u00ED aktivitu receptorov\u00E9ho kan\u00E1lu vyvolanou chemick\u00FDmi i tepeln\u00FDmi podn\u011Bty"@cs . "Molekul\u00E1rn\u00EDm klonov\u00E1n\u00EDm byl vytvo\u0159en expresn\u00ED plasmid produkuj\u00EDc\u00ED rekombinantn\u00ED b\u00EDlkovinu odvozenou od potkan\u00EDho vanilodn\u00EDho receptoru podtypu 1 TRPV1-Cdelta42. Byla optimalizov\u00E1na technologie rekombinantn\u00ED exprese tohoto mutantn\u00EDho proteinu v HEK293T bu\u0148k\u00E1ch a ov\u011B\u0159ena jeho funkce. Konstrukt byl elektrofyziologicky testov\u00E1n a prok\u00E1z\u00E1no zv\u00FD\u0161en\u00ED citlivosti k chemick\u00FDm i fyzik\u00E1ln\u00EDm podn\u011Bt\u016Fm. Proto\u017Ee TRPV1 receptor je pou\u017E\u00EDv\u00E1n jako zna\u010Dka (tzv. marker) prim\u00E1rn\u00EDch aferentn\u00EDch senzorick\u00FDch neuron\u016F s nociceptivn\u00ED charakteristikou (neuron\u016F zprost\u0159edkov\u00E1vaj\u00EDc\u00EDch informaci o bolestiv\u00FDch podn\u011Btech), v\u00FDsledn\u00FD konstrukt m\u016F\u017Ee b\u00FDt vyu\u017Eit pro charakterizaci molekul\u00E1rn\u00EDch vlastnost\u00ED a farmakologick\u00FDch \u00FA\u010Dink\u016F potenci\u00E1ln\u00EDch analgetik" . . "FGU-C" . . "P(1M0517), P(GA305/06/0319), P(LC554), Z(AV0Z50110509)" . . "Vlachov\u00E1, Viktorie" . . "2"^^ . . . "recombinant protein; vanilloid receptor subtype 1; TRP receptors"@en . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou mutac\u00ED Cdelta42" . "Odhad: 350 tis. K\u010D" . "Truncated recombinant rat vanilloid receptor subtype 1 (TRPV1- Cdelta42), with functionally approved sensitization effects on receptor-channel activity produced by chemical and thermal stimuli"@en . . "TRPV1-Cdelta42" . . . "RIV/67985823:_____/06:00324438!RIV09-AV0-67985823" . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou mutac\u00ED Cdelta42 zkracuj\u00EDc\u00ED d\u00E9lku karboxylov\u00E9ho konce, zvy\u0161uj\u00EDc\u00ED aktivitu receptorov\u00E9ho kan\u00E1lu vyvolanou chemick\u00FDmi i tepeln\u00FDmi podn\u011Bty" . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou mutac\u00ED Cdelta42 zkracuj\u00EDc\u00ED d\u00E9lku karboxylov\u00E9ho konce, zvy\u0161uj\u00EDc\u00ED aktivitu receptorov\u00E9ho kan\u00E1lu vyvolanou chemick\u00FDmi i tepeln\u00FDmi podn\u011Bty"@cs . . "The expression plasmid of recombinant protein TRPV1-Cdelta42 derived from the vanilloid receptor subtype 1 was constructed using molecular cloning methods, and the technology of its recombinant expression in HEK293T cells was optimized. The double mutant protein was functionally tested and the sensitizing effects of truncation were characterized. As TRPV1 is widely used as a universal marker for primary sensory neurons involved in pain signaling, the resulting double mutant could have a remarkable practical value for molecular and functional characterization of the effects of potential analgetics"@en . . . "Molekul\u00E1rn\u00EDm klonov\u00E1n\u00EDm byl vytvo\u0159en expresn\u00ED plasmid produkuj\u00EDc\u00ED rekombinantn\u00ED b\u00EDlkovinu odvozenou od potkan\u00EDho vanilodn\u00EDho receptoru podtypu 1 TRPV1-Cdelta42. Byla optimalizov\u00E1na technologie rekombinantn\u00ED exprese tohoto mutantn\u00EDho proteinu v HEK293T bu\u0148k\u00E1ch a ov\u011B\u0159ena jeho funkce. Konstrukt byl elektrofyziologicky testov\u00E1n a prok\u00E1z\u00E1no zv\u00FD\u0161en\u00ED citlivosti k chemick\u00FDm i fyzik\u00E1ln\u00EDm podn\u011Bt\u016Fm. Proto\u017Ee TRPV1 receptor je pou\u017E\u00EDv\u00E1n jako zna\u010Dka (tzv. marker) prim\u00E1rn\u00EDch aferentn\u00EDch senzorick\u00FDch neuron\u016F s nociceptivn\u00ED charakteristikou (neuron\u016F zprost\u0159edkov\u00E1vaj\u00EDc\u00EDch informaci o bolestiv\u00FDch podn\u011Btech), v\u00FDsledn\u00FD konstrukt m\u016F\u017Ee b\u00FDt vyu\u017Eit pro charakterizaci molekul\u00E1rn\u00EDch vlastnost\u00ED a farmakologick\u00FDch \u00FA\u010Dink\u016F potenci\u00E1ln\u00EDch analgetik"@cs . "[16C8F15A7B4B]" . "RIV/67985823:_____/06:00324438" . "497013" . . . . . "Truncated recombinant rat vanilloid receptor subtype 1 (TRPV1- Cdelta42), with functionally approved sensitization effects on receptor-channel activity produced by chemical and thermal stimuli"@en . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou mutac\u00ED Cdelta42 zkracuj\u00EDc\u00ED d\u00E9lku karboxylov\u00E9ho konce, zvy\u0161uj\u00EDc\u00ED aktivitu receptorov\u00E9ho kan\u00E1lu vyvolanou chemick\u00FDmi i tepeln\u00FDmi podn\u011Bty" .