. . . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou mutac\u00ED 3CYS" . . . . "Recombinant rat vanilloid receptor subtype 1 (TRPV1) with functionally approved triple mutation 3CYS-TRPV1, which reduces the sensitivity of the channel to reducing agents"@en . "The expression plasmid of recombinant protein 3CYS-TRPV1 derived from the vanilloid receptor subtype 1 was constructed using molecular cloning methods, and the technology of its recombinant expression in HEK293T cells was optimized. The triple mutant protein was electrophysiologically tested and its sensitivity to redox-active substances was compared with those of the wild type channel. As TRPV1 is widely used as a universal marker for primary sensory neurons involved in pain signaling, the resulting double mutant could have a remarkable practical value for molecular and functional characterization of the effects of potential analgetics"@en . "Recombinant rat vanilloid receptor subtype 1 (TRPV1) with functionally approved triple mutation 3CYS-TRPV1, which reduces the sensitivity of the channel to reducing agents"@en . . . . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou trojitou mutac\u00ED odstra\u0148uj\u00EDc\u00ED cysteinov\u00E9 zbytky exponovan\u00E9 vn\u011Bj\u0161\u00EDmu prost\u0159ed\u00ED 3CYS-TRPV1" . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou trojitou mutac\u00ED odstra\u0148uj\u00EDc\u00ED cysteinov\u00E9 zbytky exponovan\u00E9 vn\u011Bj\u0161\u00EDmu prost\u0159ed\u00ED 3CYS-TRPV1"@cs . . "2"^^ . . . "P(1M0517), P(GA305/06/0319), P(LC554), Z(AV0Z50110509)" . "Odhad: 350 tis. K\u010D" . . "Molekul\u00E1rn\u00EDm klonov\u00E1n\u00EDm byl vytvo\u0159en expresn\u00ED plasmid produkuj\u00EDc\u00ED rekombinantn\u00ED b\u00EDlkovinu odvozenou od potkan\u00EDho vanilodn\u00EDho receptoru podtypu 1 3CYS-TRPV1. Byla optimalizov\u00E1na technologie rekombinantn\u00ED exprese tohoto mutantn\u00EDho proteinu v HEK293T bu\u0148k\u00E1ch a ov\u011B\u0159ena jeho funkce. Konstrukt byl elektrofyziologicky testov\u00E1n a jeho citlivost k redox-aktivn\u00EDm l\u00E1tk\u00E1m byla porovn\u00E1na s rekombinantn\u00EDm proteinem TRPV1 divok\u00E9ho typu. Proto\u017Ee TRPV1 receptor je pou\u017E\u00EDv\u00E1n jako zna\u010Dka (tzv. marker) prim\u00E1rn\u00EDch aferentn\u00EDch senzorick\u00FDch neuron\u016F s nociceptivn\u00ED charakteristikou (neuron\u016F zprost\u0159edkov\u00E1vaj\u00EDc\u00EDch informaci o bolestiv\u00FDch podn\u011Btech), v\u00FDsledn\u00FD konstrukt m\u016F\u017Ee b\u00FDt vyu\u017Eit pro charakterizaci molekul\u00E1rn\u00EDch vlastnost\u00ED a farmakologick\u00FDch \u00FA\u010Dink\u016F potenci\u00E1ln\u00EDch analgetik" . . "recombinant protein; vanilloid receptor subtype 1; TRP receptors"@en . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou trojitou mutac\u00ED odstra\u0148uj\u00EDc\u00ED cysteinov\u00E9 zbytky exponovan\u00E9 vn\u011Bj\u0161\u00EDmu prost\u0159ed\u00ED 3CYS-TRPV1"@cs . . "Vlachov\u00E1, Viktorie" . . . "2"^^ . . . . "Teisinger, Jan" . "Molekul\u00E1rn\u00EDm klonov\u00E1n\u00EDm byl vytvo\u0159en expresn\u00ED plasmid produkuj\u00EDc\u00ED rekombinantn\u00ED b\u00EDlkovinu odvozenou od potkan\u00EDho vanilodn\u00EDho receptoru podtypu 1 3CYS-TRPV1. Byla optimalizov\u00E1na technologie rekombinantn\u00ED exprese tohoto mutantn\u00EDho proteinu v HEK293T bu\u0148k\u00E1ch a ov\u011B\u0159ena jeho funkce. Konstrukt byl elektrofyziologicky testov\u00E1n a jeho citlivost k redox-aktivn\u00EDm l\u00E1tk\u00E1m byla porovn\u00E1na s rekombinantn\u00EDm proteinem TRPV1 divok\u00E9ho typu. Proto\u017Ee TRPV1 receptor je pou\u017E\u00EDv\u00E1n jako zna\u010Dka (tzv. marker) prim\u00E1rn\u00EDch aferentn\u00EDch senzorick\u00FDch neuron\u016F s nociceptivn\u00ED charakteristikou (neuron\u016F zprost\u0159edkov\u00E1vaj\u00EDc\u00EDch informaci o bolestiv\u00FDch podn\u011Btech), v\u00FDsledn\u00FD konstrukt m\u016F\u017Ee b\u00FDt vyu\u017Eit pro charakterizaci molekul\u00E1rn\u00EDch vlastnost\u00ED a farmakologick\u00FDch \u00FA\u010Dink\u016F potenci\u00E1ln\u00EDch analgetik"@cs . "3CYS-TRPV1" . "RIV/67985823:_____/06:00324286!RIV09-AV0-67985823" . "RIV/67985823:_____/06:00324286" . "[6645A051E8C3]" . . "497014" . . . "Rekombinantn\u00ED protein potkan\u00EDho vaniloidn\u00EDho receptoru podtypu 1 (TRPV1) s funk\u010Dn\u011B ov\u011B\u0159enou trojitou mutac\u00ED odstra\u0148uj\u00EDc\u00ED cysteinov\u00E9 zbytky exponovan\u00E9 vn\u011Bj\u0161\u00EDmu prost\u0159ed\u00ED 3CYS-TRPV1" . "FGU-C" .