. "Byl vytvo\u0159en mutantn\u00ED kmen S. cerevisiae postr\u00E1daj\u00EDc\u00ED geny k\u00F3duj\u00EDc\u00ED exportn\u00ED syst\u00E9my pro kationy alkalick\u00FDch kov\u016F (NHA1, ENA1, ENA2,ENA3,ENA4) a geny pro importn\u00ED syst\u00E9my draseln\u00FDch kation\u016F. Geny NHA1 a TRK1 byly nahrazeny genem LEU2 a \u010Dty\u0159i geny ENA spolu TRK2 byly vym\u011Bn\u011Bny za gen HIS3. Vznikl tak unik\u00E1tn\u00ED GMO v genetick\u00E9m pozad\u00ED W303 s vyu\u017Eit\u00EDm pro studium heterologn\u011B exprimovan\u00FDch transport\u00E9r\u016F draseln\u00FDch kation\u016F z ni\u017E\u0161\u00EDch i vy\u0161\u0161\u00EDch eukaryot"@cs . . "Genov\u011B modifikovan\u00FD kmen S. cerevisiae postr\u00E1daj\u00EDc\u00ED v plasmatick\u00E9 membr\u00E1n\u011B exportn\u00ED i importn\u00ED syst\u00E9my pro draseln\u00E9 kationy"@cs . . "Sychrov\u00E1, Hana" . "2"^^ . "Kinclov\u00E1, Olga" . "FGU-C" . . "RIV/67985823:_____/06:00323796!RIV09-AV0-67985823" . "2"^^ . . . . . . "An S. cerevisiae mutant strain lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA3, ENA4) and potassium importers (TRK1 TRK2) has been constructed. The NHA1 and TRK1 genes have been substituted by the LEU2 gene; the four ENA genes and the TRK2 gene have been replaced by the HIS3 gene. A unique GMO strain in the W303 background has been created to study heterologously expressed potassium transporters from both lower and higher eukaryots"@en . . . "Mutantn\u00ED kmen S. cerevisiae MAB2d(nha1 ena1-4 trk1 trk2)s vysokou citlivost\u00ED k sol\u00EDm alkal. kation\u016F a s neschopnost\u00ED r\u016Fst na m\u00E9di\u00EDch s limitn\u011B n\u00EDzk\u00FDm obsah. draseln\u00FDch sol\u00ED, vhodn\u00FD pro heterologn\u00ED expresi transport\u00E9r\u016F drasl\u00EDku a sod\u00EDku z jin\u00FDch eukaryot" . "Genov\u011B modifikovan\u00FD kmen S. cerevisiae postr\u00E1daj\u00EDc\u00ED v plasmatick\u00E9 membr\u00E1n\u011B exportn\u00ED i importn\u00ED syst\u00E9my pro draseln\u00E9 kationy"@cs . . "yeast heterologous expression; S. cerevisiae; potassium sensitive mutant"@en . . . "P(IAA5011407), P(LC531), Z(AV0Z50110509)" . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium cations" . . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium cations" . . . . . "An S. cerevisiae mutant strain lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA3, ENA4) and potassium importers (TRK1 TRK2) has been constructed. The NHA1 and TRK1 genes have been substituted by the LEU2 gene; the four ENA genes and the TRK2 gene have been replaced by the HIS3 gene. A unique GMO strain in the W303 background has been created to study heterologously expressed potassium transporters from both lower and higher eukaryots" . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium cations"@en . . . "Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium cations"@en . "476879" . "RIV/67985823:_____/06:00323796" . "n\u00E1klady 500 000 K\u010D, licen\u010Dn\u00ED poplatek 50 000 K\u010D" . . "[3F1DADC10A6D]" . "S. cerevisiae MAB2d" . .