"Z(AV0Z50110509)" . "7"^^ . "potassium channel; Saccharomyces cerevisiae; salt sensitivity"@en . . "Nov\u00E9 fenotypy funk\u010Dn\u00ED exprese kan\u00E1lu mKir2.1 v kmenech Saccharomyces cerevisiae postr\u00E1daj\u00EDc\u00EDch vlastn\u00ED syst\u00E9my pro v\u00FDstup drasl\u00EDku"@cs . "532736" . "Funk\u010Dn\u00ED exprese my\u0161\u00EDho kan\u00E1lu mKir2.1 uk\u00E1zala vhodnost mutantn\u00EDch kmen\u016F S. cerevisiae postr\u00E1daj\u00EDc\u00EDch transport\u00E9ry drasl\u00EDku. Kombinace delec\u00ED gen\u016F pro transport drasl\u00EDku dovnit\u0159 a z bun\u011Bk (ena1-4delta nha1delta trk1delta trk2delta) umo\u017E\u0148uje sledovat fenotyp funk\u010Dn\u00ED exprese na dvou \u00FArovn\u00EDch \u2013 jako zv\u00FD\u0161en\u00ED citlivosti p\u0159i r\u016Fstu na vysok\u00FDch vn\u011Bj\u0161\u00EDch koncentrac\u00EDch KCl, a jako zlep\u0161en\u00ED r\u016Fstu na n\u00EDzk\u00FDch limitn\u00EDch koncentrac\u00EDch KCl. Pou\u017Eit\u00ED dele\u010Dn\u00EDho kmene kvasinek se ukazuje b\u00FDt vhodn\u00FDm n\u00E1strojem pro charakterizaci vlastnost\u00ED heterologn\u011B exprimovan\u00FDch transport\u00E9r\u016F zaji\u0161\u0165uj\u00EDc\u00EDch jak v\u00FDstup tak vstup kation\u016F alkalick\u00FDch kov\u016F do bun\u011Bk"@cs . "Hasenbrink, G." . . "New phenotypes of functional expression of the mKir2.1 channel in potassium efflux-deficient Saccharomyces cerevisiae strains" . "0749-503X" . . . "New phenotypes of functional expression of the mKir2.1 channel in potassium efflux-deficient Saccharomyces cerevisiae strains"@en . "9"^^ . "Sychrov\u00E1, Hana" . . . . . "Kol\u00E1\u010Dn\u00E1, Lucie" . . . "New phenotypes of functional expression of the mKir2.1 channel in potassium efflux-deficient Saccharomyces cerevisiae strains" . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "RIV/67985823:_____/05:00025066!RIV06-AV0-67985823" . . "3"^^ . "The use of a mutant strain lacking both potassium efflux and uptake transporters (ena1-4DELTA nha1DELTA trk1DELTA trk2DELTA) enabled the monitoring of mKir2.1 channel activity on two levels \u2013 the provision of the necessary amount of intracellular K+ in media with low potassium concentrations, and simultaneously channel\u2019s contribution to cell potassium sensitivity in the presence of high external K+. This combination of mutations proved to be new, sensitive and practical tool to characterize the properties of heterologously expressed transporters mediating both the efflux and influx of alkali metal cations"@en . "Yeast" . "Nov\u00E9 fenotypy funk\u010Dn\u00ED exprese kan\u00E1lu mKir2.1 v kmenech Saccharomyces cerevisiae postr\u00E1daj\u00EDc\u00EDch vlastn\u00ED syst\u00E9my pro v\u00FDstup drasl\u00EDku"@cs . . "Schwarzer, S." . "1315;1323" . "16" . . "Zimmermannov\u00E1, Olga" . . "The use of a mutant strain lacking both potassium efflux and uptake transporters (ena1-4DELTA nha1DELTA trk1DELTA trk2DELTA) enabled the monitoring of mKir2.1 channel activity on two levels \u2013 the provision of the necessary amount of intracellular K+ in media with low potassium concentrations, and simultaneously channel\u2019s contribution to cell potassium sensitivity in the presence of high external K+. This combination of mutations proved to be new, sensitive and practical tool to characterize the properties of heterologously expressed transporters mediating both the efflux and influx of alkali metal cations" . "22" . . "[2223B5150B01]" . "New phenotypes of functional expression of the mKir2.1 channel in potassium efflux-deficient Saccharomyces cerevisiae strains"@en . "Lichtenberg-Frat\u00E9, H." . "Ludwig, J." . "RIV/67985823:_____/05:00025066" .