"603023" . "34;49" . "Moon, H. E." . "0022-3042" . "RIV/67985823:_____/03:20030014!RIV/2004/MSM/A20004/N" . "Novotn\u00FD, Ji\u0159\u00ED" . "Low-density membrane fragments (domains) were separated from the bulk plasma membranes of HEK293 cells expressing DOP receptor-Gi1alpha fusion protein by drastic homogenization and flotation on sucrose density gradient. Low-density domains exhibited four times higher agonist-stimulated GTPase activity and GTPgammaS binding than the bulk plasma membranes (when corrected for expression levels of the receptor). RGS1 enhanced further the G protein functional activity but did not remove the difference between domain-bound and plasma membrane pools of G protein. By contrast, detergent-extraction of membrane domains diminished functional coupling between G protein\u016Fcoupled receptor and corresponding G protein." . "Svoboda, Petr" . "85" . . . "Bou\u0159ov\u00E1, Lenka" . "0"^^ . . "0"^^ . "1" . . . "Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains." . "Ko\u0161trnov\u00E1, Alexandra" . . . . "6"^^ . . "Journal of Neurochemistry" . . "8"^^ . . "Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains." . "P(GA309/01/0255), P(LN00A026), Z(AV0Z5011922), Z(MSM 113100003)" . "Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains."@en . . "RIV/67985823:_____/03:20030014" . . "[1ED3B5F0F943]" . . "Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains."@en . . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Hejnov\u00E1, Lucie" . "Milligan, G." . . "16"^^ . "Low-density membrane fragments (domains) were separated from the bulk plasma membranes of HEK293 cells expressing DOP receptor-Gi1alpha fusion protein by drastic homogenization and flotation on sucrose density gradient. Low-density domains exhibited four times higher agonist-stimulated GTPase activity and GTPgammaS binding than the bulk plasma membranes (when corrected for expression levels of the receptor). RGS1 enhanced further the G protein functional activity but did not remove the difference between domain-bound and plasma membrane pools of G protein. By contrast, detergent-extraction of membrane domains diminished functional coupling between G protein\u016Fcoupled receptor and corresponding G protein."@en . . . . . "membrane domains; GTPase activity; G protein coupling"@en . . "Moravcov\u00E1, Zuzana" . .