. "Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration" . "66" . "Urban, Otmar" . "I, P(ED1.1.00/02.0073), P(IAA600870701), P(LM2010007), Z(AV0Z60870520)" . "0366-6352" . "Rubisco content; Rubisco activity; seasonal changes; elevated CO2 concentrations; Fagus sylvatica; Picea abies"@en . . . . "[B7629D7306C6]" . . "RIV/67179843:_____/12:00379290!RIV13-AV0-67179843" . . "1"^^ . "Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration" . "3"^^ . . "Hrstka, M." . . . . "000305563300006" . . . "10.2478/s11696-012-0188-5" . "RIV/67179843:_____/12:00379290" . . "167113" . . . . "6"^^ . "Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration"@en . . . "9" . . . "Chemical Papers" . "We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species - broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mu mol mol(-1)) and elevated (E = 700 mu mol mol(-1)) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2."@en . "SK - Slovensk\u00E1 republika" . . "Seasonal changes of Rubisco content and activity in Fagus sylvatica and Picea abies affected by elevated CO2 concentration"@en . . "We studied Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) content and activity in juvenile tree species - broadleaved Fagus sylvatica L. and coniferous Picea abies (L.) Karsten exposed for three growing seasons to ambient (A = 385 mu mol mol(-1)) and elevated (E = 700 mu mol mol(-1)) CO2 concentrations. Rubisco content was determined by the SDS-PAGE method, Rubisco activity was assayed spectrophotometrically. The highest content of Rubisco enzyme in F. sylvatica was measured immediately after full leaf development followed by a gradual decrease throughout the growing season. By contrast, Rubisco content in P. abies increased markedly during the growing season. In both tree species, down-regulation of Rubisco content in trees cultivated under elevated CO2 concentration was observed. Rubisco activity was stimulated in F. sylvatica by E treatment but not in P. abies. Because no significant differences were found in Rubisco activation state between A and E, we assume that stimulation of Rubisco activity in E is not a consequence of higher carbamylation but could be caused by the release of inhibitors from active sites of Rubisco under elevated CO2." . . "Bab\u00E1k, L." . .