. . . "CZ - \u010Cesk\u00E1 republika" . "Tato pr\u00E1ce porovn\u00E1v\u00E1 pou\u017Eit\u00ED polymerasov\u00E9 \u0159et\u011Bzov\u00E9 reakce (PCR) se standardn\u00ED kultiva\u010Dn\u00ED metodou CSN/ISO/10272 pro stanoven\u00ED Campylobacter spp. v potravin\u00E1ch obsahuj\u00EDc\u00EDch ku\u0159e\u010D\u00ED maso a ur\u010Den\u00FDch k p\u0159\u00EDm\u00E9 spot\u0159eb\u011B. Pro PCR se pou\u017Eily primery specifick\u00E9 pro C. jejuni, C. coli, C. lari a intern\u00ED kontrola. Stanoven\u00ED kampylobakter\u016F bylo prov\u00E1d\u011Bno jak v neo\u0161et\u0159en\u00FDch vzorc\u00EDch tak tak\u00E9 v um\u011Ble kontaminovan\u00FDch vzorc\u00EDch potravin zakoupen\u00FDch v maloobchodn\u00ED s\u00EDti. Detek\u010Dn\u00ED limit PCR byl 2 KTJ/g po 48h kultivaci v buj\u00F3nu Parka a Sanderse. Duplex PCR se uk\u00E1zal b\u00FDt vysoce spolehliv\u00FD pro stanoven\u00ED kampylobakter\u016F v r\u016Fzn\u00FDch typech potravin. I bez um\u011Bl\u00E9 kontaminace vyk\u00E1zaly vzorky z celosv\u011Btov\u00E9ho \u0159et\u011Bzce s rychl\u00FDm ob\u010Derstven\u00EDm po PCR amplifikaci pozitivn\u00ED produkt zat\u00EDmco v\u00FDsledky kultivac\u00ED stejn\u00FDch vzork\u016F byly negativn\u00ED."@cs . "1"^^ . "[766F6EDBCDC9]" . . "Z(AV0Z60870520), Z(MSM6046137305)" . . "1212-1800" . . . "polymerase chain reaction; internal control; Campylobacter spp."@en . "This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken meat. PCR was carried out with the primers specific to C. jejuni, C. coli, C. lari, and was modified with an internal control. The detection of campylobacters by PCR was performed on both untreated and spiked samples of real food purchased in local stores. For PCR, the detection limit was 2 CFU/g after 48 h enrichment in Park and Sanders broth. Duplex PCR proved to be highly reliable in the detection of campylobacters in different food types. Without extra spiking, samples from a global fast food chain exhibited positive amplification of the PCR product while but negative results were obtained from the cultivation of the same samples."@en . . "Optimalizace PCR metody pro stanoven\u00ED Campylobacter jejuni a Campylobacter coli ve vzorc\u00EDch potravin obsahuj\u00EDc\u00EDch ku\u0159ec\u00ED maso a ur\u010Den\u00FDch k p\u0159\u00EDm\u00E9 spot\u0159eb\u011B"@cs . "Czech Journal of Food Science" . "3"^^ . . . "Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals"@en . . "Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals" . . "26" . "RIV/67179843:_____/08:00312083" . "7"^^ . . . "Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals"@en . "Optimalizace PCR metody pro stanoven\u00ED Campylobacter jejuni a Campylobacter coli ve vzorc\u00EDch potravin obsahuj\u00EDc\u00EDch ku\u0159ec\u00ED maso a ur\u010Den\u00FDch k p\u0159\u00EDm\u00E9 spot\u0159eb\u011B"@cs . . "RIV/67179843:_____/08:00312083!RIV09-AV0-67179843" . "Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals" . "000259006100008" . "-" . "\u0160abatkov\u00E1, Zde\u0148ka" . "This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken meat. PCR was carried out with the primers specific to C. jejuni, C. coli, C. lari, and was modified with an internal control. The detection of campylobacters by PCR was performed on both untreated and spiked samples of real food purchased in local stores. For PCR, the detection limit was 2 CFU/g after 48 h enrichment in Park and Sanders broth. Duplex PCR proved to be highly reliable in the detection of campylobacters in different food types. Without extra spiking, samples from a global fast food chain exhibited positive amplification of the PCR product while but negative results were obtained from the cultivation of the same samples." . "Demnerov\u00E1, K." . "Pazlarov\u00E1, J." . "385199" . .