. . . . "Neoplasma" . "CZ - \u010Cesk\u00E1 republika" . "Both CD 8+ and CD 4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation snd proliferation of T cells require cellular interactions including adhesion,recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). Dcs werwobtained from peripheral blood mononuclear cells (PBMC) in the presence of IL ? 4 and GM-CSF. TNF ?\u00E1 was added to induce maturation. A conjugate of myeloma idiotypic protein wih keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of IL-2 and IL-7. Autologous DCs were added to the lymphocyte on days 3,10, and17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in response to anit"@en . . "Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2" . "50" . "RIV/65269705:_____/03:00007983" . "9"^^ . "120-123" . . "9"^^ . . . "5" . "Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2"@en . . "Both CD 8+ and CD 4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation snd proliferation of T cells require cellular interactions including adhesion,recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). Dcs werwobtained from peripheral blood mononuclear cells (PBMC) in the presence of IL ? 4 and GM-CSF. TNF ?\u00E1 was added to induce maturation. A conjugate of myeloma idiotypic protein wih keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of IL-2 and IL-7. Autologous DCs were added to the lymphocyte on days 3,10, and17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in response to anit" . . "0028-2685" . "0"^^ . "[6E5FB342AAD5]" . "0"^^ . . "Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2" . "4"^^ . "B\u00FCchler, Tom\u00E1\u0161" . "RIV/65269705:_____/03:00007983!RIV/2004/MZ0/L43004/N" . . . "P(NC6763)" . "Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2"@en . . "T cells;dendritic cells;immunotherapy;in vitor cell culture"@en . . . "613910" .