. "RIV/62157124:16270/08:00001773!RIV09-MSM-16270___" . "Stejskal, Karel" . "Beklov\u00E1, Miroslava" . "metallothionein; bio-asssessing; electrochemistry"@en . . . "357992" . "16270" . "Horna, Ale\u0161" . "Bio-hodnocen\u00ED zne\u010Di\u0161t\u011Bn\u00ED prost\u0159ed\u00ED pomoc\u00ED sledov\u00E1n\u00ED hladiny metalothioneinu u\u017Eit\u00EDm elektrochemick\u00E9 detekce"@cs . . . "Trnkov\u00E1, Libu\u0161e" . "Hanu\u0161tiak, Pavel" . "K\u0159\u00ED\u017Ekov\u00E1, So\u0148a" . . "2"^^ . "IEEE Sensors Journal" . "Bio-hodnocen\u00ED zne\u010Di\u0161t\u011Bn\u00ED prost\u0159ed\u00ED pomoc\u00ED sledov\u00E1n\u00ED hladiny metalothioneinu u\u017Eit\u00EDm elektrochemick\u00E9 detekce"@cs . . "Kizek, Ren\u00E9" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Bio-Assesing of environmental pollution via monitoring of metallothionein level using electrochemical detection."@en . "Adam, Vojt\u011Bch" . "C\u00EDlem t\u00E9to pr\u00E1ce bylo zkoum\u00E1n\u00ED vlivu st\u0159\u00EDbrn\u00FDch iont\u016F (0, 0.3, 0.6, 1.2, 2.5 and 5 uM)na rybu pav\u00ED o\u010Dko (Poecilia reticulate), podle dob\u0159e \u0159\u00EDzen\u00FDch experiment\u00E1ln\u00EDch podm\u00EDnek po sedm dn\u00ED. Pro pochopen\u00ED fyziologick\u00FDch zm\u011Bn jsme se sna\u017Eili stanovit metalothionein (MT) jako biomarker stresu z vystaven\u00ED t\u011B\u017Ek\u00FDm kov\u016Fm. Pro tyto \u00FA\u010Dely jsme navrhli nov\u00FD sensor pou\u017E\u00EDvaj\u00EDd\u00ED uhl\u00EDkovou elektrodu s pr\u016Ftokovou injek\u010Dn\u00ED anal\u00FDzou. Nejprve jsme optimalizovali navrhnut\u00FD senzor. Optim\u00E1ln\u00ED podm\u00EDnky pokusu byly n\u00E1sleduj\u00EDc\u00ED - potenci\u00E1l: 750 mV, mobiln\u00ED f\u00E1ze: Britton-Robinson pufr (pH 1.9) s rychlost\u00ED pr\u016Ftoku 0.6 ml/min, \u010Dasov\u00FD filtr: 2,5 s, %22current R%22: 1 uA. Za t\u011Bchto ppodm\u00EDnek byl detek\u010Dn\u00ED limit odhadnut 100 nM MT. Potom n\u00E1sledovala anal\u00FDza ryb\u00ED tk\u00E1n\u011B. Na z\u00E1klad\u011B obdr\u017Een\u00FDch v\u00FDsledk\u016F, byla koncentrace MT zv\u00FD\u0161en\u00E1 se zvy\u0161uj\u00EDc\u00ED se d\u00E1vkou st\u0159\u00EDbrn\u00FDch iont\u016F a d\u00E9lkou vystaven\u00ED st\u0159\u00EDbrn\u00FDm iont\u016Fm. V\u00FDsledky korelovali s adsorptivn\u00ED p\u0159enosovou rozpou\u0161t\u011Bc\u00ED technikou s diferen\u010Dn\u00ED pulzn\u00ED voltametri\u00ED - Brdi\u010Dkova reakce, kter\u00E9 by"@cs . "RIV/62157124:16270/08:00001773" . . "Bio-Assesing of environmental pollution via monitoring of metallothionein level using electrochemical detection." . "In the present work we reports on investigations of affecting of guppy fishes (Poecilia reticulate) by silver ions (0, 0.3, 0.6, 1.2, 2.5 and 5 uM) under well controlled experimental conditions for seven days. To follow the physiological changes we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For these purposes we suggested a new sensor utilizing carbon electrode with flow injection analysis. Primarily, we optimized the suggested sensor. The experimental conditions optimized were as follows - applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with flow rate of 0.6 ml/min, time filter: 2.5 s, %22current R%22: 1 uA. Under these conditions the detection limit was estimated as 100 nM of MT. After the optimizing step, the analysis of fish tissues followed. Based on the results obtained, MT concentration increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained b" . . . "Z(MSM6215712402)" . . "1530-437X" . . "Sures, Bernd" . "Hub\u00E1lek, Jarom\u00EDr" . "Bio-Assesing of environmental pollution via monitoring of metallothionein level using electrochemical detection." . "Bio-Assesing of environmental pollution via monitoring of metallothionein level using electrochemical detection."@en . "12"^^ . "8" . . "Zehn\u00E1lek, Josef" . . "Svobodov\u00E1, Zde\u0148ka" . "[DFC9E22DA227]" . . "In the present work we reports on investigations of affecting of guppy fishes (Poecilia reticulate) by silver ions (0, 0.3, 0.6, 1.2, 2.5 and 5 uM) under well controlled experimental conditions for seven days. To follow the physiological changes we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For these purposes we suggested a new sensor utilizing carbon electrode with flow injection analysis. Primarily, we optimized the suggested sensor. The experimental conditions optimized were as follows - applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with flow rate of 0.6 ml/min, time filter: 2.5 s, %22current R%22: 1 uA. Under these conditions the detection limit was estimated as 100 nM of MT. After the optimizing step, the analysis of fish tissues followed. Based on the results obtained, MT concentration increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained b"@en . "8"^^ . .