"Li\u0161kov\u00E1, Marcela" . "Bioluminescence determination of active caspase-3 in single apoptotic cells"@en . "12" . "Klep\u00E1rn\u00EDk, Karel" . "Hegrov\u00E1, Jitka" . "Foret, Franti\u0161ek" . "10.1002/elps.201200675" . . "I, P(GAP206/11/2377), P(GAP502/12/1285)" . . . "Bioluminescence determination of active caspase-3 in single apoptotic cells"@en . "Bioluminescence determination of active caspase-3 in single apoptotic cells" . "Caspase-3 is an executive caspase, in the central position within apoptotic machinery. Investigation of this molecules is of a great importance for basic as well as applied research. In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. Three different instrumental arrangements were compared for the determination of caspase-3 in cells. The best setting of the miniaturized system detected the mean amount as low as about 6.5 fg corresponding to 122000 molecules of caspase-3 in individual apoptotic cells."@en . "7"^^ . . . . "P\u0159ikryl, Jan" . . "63488" . "16170" . "Electrophoresis" . . "1"^^ . . . "RIV/62157124:16170/13:43871765!RIV14-GA0-16170___" . . "Bioluminescence determination of active caspase-3 in single apoptotic cells" . . . "Caspase-3 is an executive caspase, in the central position within apoptotic machinery. Investigation of this molecules is of a great importance for basic as well as applied research. In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. Three different instrumental arrangements were compared for the determination of caspase-3 in cells. The best setting of the miniaturized system detected the mean amount as low as about 6.5 fg corresponding to 122000 molecules of caspase-3 in individual apoptotic cells." . "\u0160vandov\u00E1, Eva" . "000320385400009" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "0173-0835" . "6"^^ . "bioluminiscence; caspase 3"@en . "[D92FCBA0CA0E]" . . . "34" . "Matalov\u00E1, Eva" . . "RIV/62157124:16170/13:43871765" .