"The quantitative analysis of the histological changes in seminiferous epithelium of tomcats during the reversible suppression of sexual activity with GnRH agonist deslorelin"@en . . . . "2012-05-30+02:00"^^ . . . "6"^^ . . "V projektu bylo pou\u017Eito 5 kocour\u016F v kontroln\u00ED skupin\u011B (Skupina 1) a dal\u0161\u00EDch 22 kocour\u016F, kter\u00FDm byl zaveden subkut\u00E1nn\u011B implant\u00E1t do oblasti prav\u00E9 lopatky, bylo n\u00E1sledn\u011B rozd\u011Bleno do dvou skupin. Zv\u00ED\u0159ata ve Skupin\u011B 2 (n=14) byla pozorov\u00E1na ode dne implantace a sledov\u00E1n\u00ED trvalo 4 m\u011Bs\u00EDce. Ve skupin\u011B 3 (n=8) byla v\u0161echna zv\u00ED\u0159ata sledov\u00E1na od data zaveden\u00ED implant\u00E1tu. Po \u010Dty\u0159ech m\u011Bs\u00EDc\u00EDch od zaveden\u00ED byly kocour\u016Fm ve Skupin\u011B 3 implant\u00E1ty vyjmuty a zv\u00ED\u0159ata byla monitorov\u00E1na dal\u0161\u00ED \u010Dty\u0159i m\u011Bs\u00EDce. U v\u0161ech kocour\u016F byla provedena p\u0159i prvn\u00ED kontrole a n\u00E1sledn\u011B v m\u011Bs\u00ED\u010Dn\u00EDch intervalech n\u00E1sleduj\u00EDc\u00ED vy\u0161et\u0159en\u00ED: odb\u011Br krve a m\u011B\u0159en\u00ED hladiny testosteronu p\u0159ed (T0) a po aplikaci (T4) hum\u00E1nn\u00EDho choriov\u00E9ho gonadotropinu (HCG), m\u011B\u0159en\u00ED velikosti varlat. U v\u0161ech kocour\u016F byl proveden odb\u011Br ejakul\u00E1tu v pr\u016Fb\u011Bhu prvn\u00ED n\u00E1v\u0161t\u011Bvy a pot\u00E9 opakovan\u011B ve dvoum\u011Bs\u00ED\u010Dn\u00EDch intervalech a\u017E do konce sledov\u00E1n\u00ED. U ka\u017Ed\u00E9ho ejakul\u00E1tu byl stanoven celkov\u00FD po\u010Det spermi\u00ED. Dva a\u017E \u010Dty\u0159i kocou\u0159i byli kastrov\u00E1n\u00ED v t\u00FDdnech 4, 8, 12, 16, 20, 24, 28 a 32 a bylo provedeno histologick\u00E9 vy\u0161et\u0159en\u00ED varlat. Zhodnocen\u00EDm 200 p\u0159\u00ED\u010Dn\u00FDch pr\u016F\u0159ez\u016F semenotvorn\u00FDmi kan\u00E1lky byl ur\u010Den stupe\u0148 suprese spermatogeneze a zv\u00ED\u0159ata ve skupin\u00E1ch 2 a 3 byla rozd\u011Blena do skupin podle v\u011Bt\u0161inov\u00E9ho v\u00FDskytu tubul\u016F s nejvyvinut\u011Bj\u0161\u00EDmi z\u00E1rode\u010Dn\u00FDmi bu\u0148kami n\u00E1sledovn\u011B: G1, spermatocyty; G2, okrouhl\u00E9 spermatidy; G3, elonguj\u00EDc\u00ED se spermatidy a G4, elongovan\u00E9 spermatidy. Byla spo\u010D\u00EDt\u00E1na pr\u016Fm\u011Brn\u00E1 plocha jader Leydigov\u00FDch bun\u011Bk. U zv\u00ED\u0159at ve Skupin\u011B 2 byla sledov\u00E1na suprese pohlavn\u00ED aktivity po zaveden\u00ED implant\u00E1tu. Do\u0161lo k poklesu koncentrace T4, velikosti varlat a celkov\u00E9ho po\u010Dtu spermi\u00ED v ejakul\u00E1tu (P { 0.01; P { 0.01; respektive P { 0.05) do \u010Dty\u0159 m\u011Bs\u00EDc\u016F po implantaci. Histologick\u00E9 posouzen\u00ED prok\u00E1zalo vysokou individu\u00E1ln\u00ED variabilitu ve stupni suprese spermatogeneze. U zv\u00ED\u0159at ve Skupin\u011B 3 byl implant\u00E1t \u010Dty\u0159i m\u011Bs\u00EDce po zaveden\u00ED vyjmut a byl monitorov\u00E1n n\u00E1vrat pohlavn\u00ED aktivity. Do \u010Dty\u0159 m\u011Bs\u00EDc\u016F vyjmut\u00ED dos\u00E1hla koncentrace T4 a celkov\u00E9ho po\u010Det spermi\u00ED v ejakul\u00E1tu hodno"@cs . "16170" . "Kvantitativn\u00ED anal\u00FDza histologick\u00FDch zm\u011Bn semenotvorn\u00E9ho epitelu varlat kocour\u016F p\u0159i reverzibiln\u00ED supresi pohlavn\u00ED aktivity gnrh agonistou deslorelinem" . . "Novotn\u00FD, Ladislav" . . "Kvantitativn\u00ED anal\u00FDza histologick\u00FDch zm\u011Bn semenotvorn\u00E9ho epitelu varlat kocour\u016F p\u0159i reverzibiln\u00ED supresi pohlavn\u00ED aktivity gnrh agonistou deslorelinem" . "3"^^ . "[CAC6F59948B4]" . "Jano\u0161ovsk\u00E1, Michaela" . . . "Using a control group (Group 1, n = 5), 22 domestic tomcats were given the implant subcutaneously in the region of the right shoulder blade and were then divided into two treatment groups. Animals in Group 2 (n = 14) were observed from the date of implant surgery and the observation lasted for four months. In Group 3 (n = 8) all animals were monitored from the date of implant surgery. Then, after four months, all implants were removed and the toms were observed for a further four months. In all animals during their first visit and then in one-month intervals, changes in testosterone concentrations were assessed before (T0) and four hours after (T4) human chorionic gonadotropin (HCG) administration and testis size was measured. In all tomcats, semen collection was performed, using an electroejaculator, in the course of the first visit and then in two-month intervals or at the end of observation. Total sperm count was determined in each semen sample. Two to four animals were castrated at weeks 4, 8, 12, 16, 20, 24, 28 and 32 and histological assessment of the testes was performed. By evaluation of 200 cross-sections of seminiferous tubules, the degree of spermatogenic suppression was assessed and animals in groups 2 and 3 were assigned into groups according to the majority of tubules with the most developed germ cell observed: G1, spermatocytes; G2, round spermatids; G3, elongating spermatids and G4, elongated spermatids. The mean area of Leydig-cell nuclei was calculated. In animals in Group 2, suppression after implant insertion was monitored. T4 concentrations, testis size, and total sperm count gradually decreased (P { 0.01; P { 0.01; and P { 0.05 respectively) within four months after implantation. Histological evaluation showed a high individual variation in the degree of suppression of spermatogenesis. In animals in Group 3, the implant was removed four months after insertion and the return of sexual activity was monitored."@en . . . "The quantitative analysis of the histological changes in seminiferous epithelium of tomcats during the reversible suppression of sexual activity with GnRH agonist deslorelin"@en . "Kvantitativn\u00ED anal\u00FDza histologick\u00FDch zm\u011Bn semenotvorn\u00E9ho epitelu varlat kocour\u016F p\u0159i reverzibiln\u00ED supresi pohlavn\u00ED aktivity gnrh agonistou deslorelinem"@cs . "Sborn\u00EDk p\u0159\u00EDsp\u011Bvk\u016F - Konference mlad\u00FDch v\u011Bdeck\u00FDch pracovn\u00EDk\u016F s mezin\u00E1rodn\u00ED \u00FA\u010Dast\u00ED 2012 na FVL VFU Brno" . "Kvantitativn\u00ED anal\u00FDza histologick\u00FDch zm\u011Bn semenotvorn\u00E9ho epitelu varlat kocour\u016F p\u0159i reverzibiln\u00ED supresi pohlavn\u00ED aktivity gnrh agonistou deslorelinem"@cs . "Vit\u00E1sek, Roman" . . "Veterin\u00E1rn\u00ED a farmaceutick\u00E1 univerzita Brno" . "S" . . "RIV/62157124:16170/12:43871352!RIV13-MSM-16170___" . "Barto\u0161kov\u00E1, Alena" . . "V projektu bylo pou\u017Eito 5 kocour\u016F v kontroln\u00ED skupin\u011B (Skupina 1) a dal\u0161\u00EDch 22 kocour\u016F, kter\u00FDm byl zaveden subkut\u00E1nn\u011B implant\u00E1t do oblasti prav\u00E9 lopatky, bylo n\u00E1sledn\u011B rozd\u011Bleno do dvou skupin. Zv\u00ED\u0159ata ve Skupin\u011B 2 (n=14) byla pozorov\u00E1na ode dne implantace a sledov\u00E1n\u00ED trvalo 4 m\u011Bs\u00EDce. Ve skupin\u011B 3 (n=8) byla v\u0161echna zv\u00ED\u0159ata sledov\u00E1na od data zaveden\u00ED implant\u00E1tu. Po \u010Dty\u0159ech m\u011Bs\u00EDc\u00EDch od zaveden\u00ED byly kocour\u016Fm ve Skupin\u011B 3 implant\u00E1ty vyjmuty a zv\u00ED\u0159ata byla monitorov\u00E1na dal\u0161\u00ED \u010Dty\u0159i m\u011Bs\u00EDce. U v\u0161ech kocour\u016F byla provedena p\u0159i prvn\u00ED kontrole a n\u00E1sledn\u011B v m\u011Bs\u00ED\u010Dn\u00EDch intervalech n\u00E1sleduj\u00EDc\u00ED vy\u0161et\u0159en\u00ED: odb\u011Br krve a m\u011B\u0159en\u00ED hladiny testosteronu p\u0159ed (T0) a po aplikaci (T4) hum\u00E1nn\u00EDho choriov\u00E9ho gonadotropinu (HCG), m\u011B\u0159en\u00ED velikosti varlat. U v\u0161ech kocour\u016F byl proveden odb\u011Br ejakul\u00E1tu v pr\u016Fb\u011Bhu prvn\u00ED n\u00E1v\u0161t\u011Bvy a pot\u00E9 opakovan\u011B ve dvoum\u011Bs\u00ED\u010Dn\u00EDch intervalech a\u017E do konce sledov\u00E1n\u00ED. U ka\u017Ed\u00E9ho ejakul\u00E1tu byl stanoven celkov\u00FD po\u010Det spermi\u00ED. Dva a\u017E \u010Dty\u0159i kocou\u0159i byli kastrov\u00E1n\u00ED v t\u00FDdnech 4, 8, 12, 16, 20, 24, 28 a 32 a bylo provedeno histologick\u00E9 vy\u0161et\u0159en\u00ED varlat. Zhodnocen\u00EDm 200 p\u0159\u00ED\u010Dn\u00FDch pr\u016F\u0159ez\u016F semenotvorn\u00FDmi kan\u00E1lky byl ur\u010Den stupe\u0148 suprese spermatogeneze a zv\u00ED\u0159ata ve skupin\u00E1ch 2 a 3 byla rozd\u011Blena do skupin podle v\u011Bt\u0161inov\u00E9ho v\u00FDskytu tubul\u016F s nejvyvinut\u011Bj\u0161\u00EDmi z\u00E1rode\u010Dn\u00FDmi bu\u0148kami n\u00E1sledovn\u011B: G1, spermatocyty; G2, okrouhl\u00E9 spermatidy; G3, elonguj\u00EDc\u00ED se spermatidy a G4, elongovan\u00E9 spermatidy. Byla spo\u010D\u00EDt\u00E1na pr\u016Fm\u011Brn\u00E1 plocha jader Leydigov\u00FDch bun\u011Bk. U zv\u00ED\u0159at ve Skupin\u011B 2 byla sledov\u00E1na suprese pohlavn\u00ED aktivity po zaveden\u00ED implant\u00E1tu. Do\u0161lo k poklesu koncentrace T4, velikosti varlat a celkov\u00E9ho po\u010Dtu spermi\u00ED v ejakul\u00E1tu (P { 0.01; P { 0.01; respektive P { 0.05) do \u010Dty\u0159 m\u011Bs\u00EDc\u016F po implantaci. Histologick\u00E9 posouzen\u00ED prok\u00E1zalo vysokou individu\u00E1ln\u00ED variabilitu ve stupni suprese spermatogeneze. U zv\u00ED\u0159at ve Skupin\u011B 3 byl implant\u00E1t \u010Dty\u0159i m\u011Bs\u00EDce po zaveden\u00ED vyjmut a byl monitorov\u00E1n n\u00E1vrat pohlavn\u00ED aktivity. Do \u010Dty\u0159 m\u011Bs\u00EDc\u016F vyjmut\u00ED dos\u00E1hla koncentrace T4 a celkov\u00E9ho po\u010Det spermi\u00ED v ejakul\u00E1tu hodno" . . "RIV/62157124:16170/12:43871352" . . . "Brno" . . "\u010C\u00ED\u017Eek, Petr" . "Brno" . . "Histological evaluation; Semen collection; Testis size; Male cat; GnRH-agonist"@en . "978-80-7305-620-9" . "146156" . "P\u0159inosilov\u00E1, Petra" . . "5"^^ . .