. "The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p { 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p { 0.05) in experiment 1. The fluid from ovarian cysts } 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO(3), BE, pCO(2) and pO(2) (experiment 2). The indicators were compared between samples 1 and 2. Higher pO(2) as well as pH and lower pCO(2) in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration."@en . "acid-base indicators; cysts; follicles; ovum pick-up; collection of follicular fluid; heifer; Cow"@en . . . "0001-7213" . . . "Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle" . . "RIV/62157124:16170/11:43870732" . "Z(MSM6215712403)" . "CZ - \u010Cesk\u00E1 republika" . . "209573" . "Dole\u017Eel, Radovan" . . "Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle" . "Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle"@en . . "Lopat\u00E1\u0159ov\u00E1, Miloslava" . "Pechov\u00E1, Alena" . "6"^^ . "Indrov\u00E1, Eva" . . "6"^^ . "RIV/62157124:16170/11:43870732!RIV12-MSM-16170___" . . "Acta veterinaria Brno" . . "000293898400007" . . . "10.2754/avb201180020179" . . "2" . . "[49CC8AD3B9CD]" . "\u010Cech, Svatopluk" . "80" . . . . "Mal\u00E1, Jana" . . "16170" . . . "6"^^ . . . . "Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle"@en . "The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p { 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p { 0.05) in experiment 1. The fluid from ovarian cysts } 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO(3), BE, pCO(2) and pO(2) (experiment 2). The indicators were compared between samples 1 and 2. Higher pO(2) as well as pH and lower pCO(2) in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration." .