"RIV/62157124:16170/11:43870446" . "Vojtkov\u00E1, Lucy" . . . . . "3"^^ . "78" . . "209177" . . "G\u00E1l, Petr" . . . "L\u00E9\u010Dba kostn\u00EDho m\u016Fstku transplantac\u00ED mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk a chondrocyt\u016F v kompozitn\u00EDm nosi\u010Di u prasat - experiment\u00E1ln\u00ED studie." . . . "Crha, Michal" . "Proks, Pavel" . "L\u00E9\u010Dba kostn\u00EDho m\u016Fstku transplantac\u00ED mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk a chondrocyt\u016F v kompozitn\u00EDm nosi\u010Di u prasat - experiment\u00E1ln\u00ED studie."@cs . . "CZ - \u010Cesk\u00E1 republika" . "The presented experimental study describes the results of using a combination of allogeneic mesenchymal cells (MSCs) with chondrocytes (CHCs) and a novel scaffold based on type I collagen and chitosan fibres. This biocomposite was transplanted into a defect produced by excision of a bone bridge to induce new cartilaginous tissue formation. The left femur was treated by transplantation into a defect of distal epiphysis; the right femur with implantation of the scaffold only served as control. A better therapeutic result was therefore expected in the left femur - the reduction of growth and angular deformities, and the histological finding of a tissue similar to the cartilage excised from the left femur. The miniature pig was selected as an experimental model and 10 pigs were used. Mesenchymal stem cells derived from femoral bone marrow and chondrocytes derived from a sample harvested from the non-weight-bearing articular surface of the distal end of the femur were cultured in medium. The novel scaffold was based on collagen containing chitosan nano-fibres. To make manipulation during implantation easier, the cilindrical scaffolds after lyophilisation were again placed in 96-well plates for seeding. The scaffolds before implantation were seeded with 2x106 allogeneic MSCs and 1x106 allogeneic CHCs. The outcomes of treatment were assessed by measuring the length of bone and the degree of distal femoral valgus deformity, and by the histological findings obtained (properties and maturity of the newly-formed tissue, detection of type II collagen, PAS reaction). The right and left legs were examined for longitudinal bone growth and the valgus angle and compared. The treated left leg showed a higher average value for longitudinal growth than the untreated right leg (p = 0.004). The average degree of angular deformity was lower in the left leg than in the right leg (p = 0.008). The microscopic findings showed that a tissue similar to hyaline cartilage was more frequently pr"@en . . "6"^^ . "Treatment of bone bridge using transplantation of mesenchymal stem cells and chondrocytes in composite scaffold in pigs - experimental study"@en . "0001-5415" . "scaffold; bone bridge; growth plate; Mesenchymal stem cells"@en . "L\u00E9\u010Dba kostn\u00EDho m\u016Fstku transplantac\u00ED mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk a chondrocyt\u016F v kompozitn\u00EDm nosi\u010Di u prasat - experiment\u00E1ln\u00ED studie." . "6" . "Pl\u00E1nka, Ladislav" . "[D476850E85A0]" . . "L\u00E9\u010Dba kostn\u00EDho m\u016Fstku transplantac\u00ED mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk a chondrocyt\u016F v kompozitn\u00EDm nosi\u010Di u prasat - experiment\u00E1ln\u00ED studie."@cs . "Acta Chirurgiae Orthopaedicae et Traumatologiae \u010Cechosl" . . "Ne\u010Das, Alois" . . . "I, P(2B06130), P(NS9896)" . . "P\u0159edkl\u00E1dan\u00E1 experiment\u00E1ln\u00ED studie popisuje v\u00FDsledky pou\u017Eit\u00ED kombinace alogenn\u00EDch mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk (MSCs) s chondrocyty (CHC) a nov\u00E9ho nosi\u010De slo\u017Een\u00E9ho z kolagenu typu I a chitosanov\u00FDch vl\u00E1ken. Transplantace uveden\u00E9ho biokompozitu do defektu vznikl\u00E9ho po resekci kostn\u00EDho m\u016Fstku m\u011Bla indukovat tvorbu nov\u00E9 chrupav\u010Dit\u00E9 tk\u00E1n\u011B. Transplantace byla provedena do defektu dist\u00E1ln\u00ED epif\u00FDzy lev\u00E9 stehenn\u00ED kosti, prav\u00E1 slou\u017Eila jako kontrola, zde byl implantov\u00E1n samotn\u00FD nosi\u010D. Byl tedy o\u010Dek\u00E1v\u00E1n lep\u0161\u00ED terapeutick\u00FD v\u00FDsledek na lev\u00E9 stehenn\u00ED kosti - redukce r\u016Fstov\u00E9 i \u00FAhlov\u00E9 deformity a histologick\u00FD n\u00E1lez odpov\u00EDdaj\u00EDc\u00ED tvorb\u011B tk\u00E1n\u011B podobn\u00E9 chrupavce v resekovan\u00E9m defektu lev\u00E9 stehenn\u00ED kosti. Experiment\u00E1ln\u00EDm modelem bylo zvoleno miniaturn\u00ED prase, experiment\u00E1ln\u00ED skupinu tvo\u0159ilo deset zv\u00ED\u0159at. Zdrojem mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk byla zvolena kostn\u00ED d\u0159e\u0148 ky\u010Deln\u00ED kosti. Zdrojem chondrocyt\u016F byl odebran\u00FD vzorek z nez\u00E1t\u011B\u017Eov\u00E9 \u010D\u00E1sti kloubn\u00ED plochy dist\u00E1ln\u00ED epif\u00FDzy stehenn\u00ED kosti a jejich dal\u0161\u00ED kultivace v \u017Eivn\u00E9m mediu. Nov\u011B pou\u017Eit\u00FD skafold byl na b\u00E1zi kolagenu obsahuj\u00EDc\u00EDho nanovl\u00E1kna z chitosanu. Pro zjednodu\u0161en\u00ED manipulace p\u0159i implantaci byly v\u00E1lcovit\u00E9 substr\u00E1ty po lyofilizaci op\u011Bt p\u0159ed\u00E1ny k osazen\u00ED bu\u0148kami v 96-jamkov\u00E9 desti\u010Dce. V\u00E1le\u010Dky byly p\u0159ed implantac\u00ED osazeny 2x106 alogenn\u00EDmi MSC a 1x106 alogenn\u00EDmi CHC. V\u00FDsledky l\u00E9\u010Dby byly hodnoceny na z\u00E1klad\u011B m\u011B\u0159en\u00ED d\u00E9lky kost\u00ED a valg\u00F3zn\u00ED deformace dist\u00E1ln\u00ED epif\u00FDzy stehenn\u00ED kosti a na z\u00E1klad\u011B histologick\u00E9ho vy\u0161et\u0159en\u00ED (charakter a vyzr\u00E1lost novotvo\u0159en\u00E9 tk\u00E1n\u011B, detekce kolagenu II, PAS reakce). Byl sledov\u00E1n rozd\u00EDl mezi nel\u00E9\u010Denou pravou a l\u00E9\u010Denou levou kon\u010Detinou v parametrech r\u016Fst do d\u00E9lky a rozd\u00EDl v \u00FAhlu valgizace. L\u00E9\u010Den\u00E1 lev\u00E1 kon\u010Detina m\u011Bla vy\u0161\u0161\u00ED pr\u016Fm\u011Br r\u016Fstu do d\u00E9lky proti nel\u00E9\u010Den\u00E9 prav\u00E9 kon\u010Detin\u011B (p = 0,004). Pr\u016Fm\u011Brn\u00FD \u00FAhel valgizace dist\u00E1ln\u00ED epif\u00FDzy lev\u00E9 stehenn\u00ED kosti byl men\u0161\u00ED ne\u017E pr\u016Fm\u011Brn\u00FD \u00FAhel valgizace dist\u00E1ln\u00ED epif\u00FDzy prav\u00E9 stehenn\u00ED kosti (p = 0,008). V defektu lev\u00E9 dist\u00E1ln\u00ED stehenn\u00ED kosti byla \u010Dast\u011Bji nalezena tk\u00E1\u0148 bl\u00EDzk\u00E1 hyalin"@cs . . . "P\u0159edkl\u00E1dan\u00E1 experiment\u00E1ln\u00ED studie popisuje v\u00FDsledky pou\u017Eit\u00ED kombinace alogenn\u00EDch mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk (MSCs) s chondrocyty (CHC) a nov\u00E9ho nosi\u010De slo\u017Een\u00E9ho z kolagenu typu I a chitosanov\u00FDch vl\u00E1ken. Transplantace uveden\u00E9ho biokompozitu do defektu vznikl\u00E9ho po resekci kostn\u00EDho m\u016Fstku m\u011Bla indukovat tvorbu nov\u00E9 chrupav\u010Dit\u00E9 tk\u00E1n\u011B. Transplantace byla provedena do defektu dist\u00E1ln\u00ED epif\u00FDzy lev\u00E9 stehenn\u00ED kosti, prav\u00E1 slou\u017Eila jako kontrola, zde byl implantov\u00E1n samotn\u00FD nosi\u010D. Byl tedy o\u010Dek\u00E1v\u00E1n lep\u0161\u00ED terapeutick\u00FD v\u00FDsledek na lev\u00E9 stehenn\u00ED kosti - redukce r\u016Fstov\u00E9 i \u00FAhlov\u00E9 deformity a histologick\u00FD n\u00E1lez odpov\u00EDdaj\u00EDc\u00ED tvorb\u011B tk\u00E1n\u011B podobn\u00E9 chrupavce v resekovan\u00E9m defektu lev\u00E9 stehenn\u00ED kosti. Experiment\u00E1ln\u00EDm modelem bylo zvoleno miniaturn\u00ED prase, experiment\u00E1ln\u00ED skupinu tvo\u0159ilo deset zv\u00ED\u0159at. Zdrojem mezenchymov\u00FDch kmenov\u00FDch bun\u011Bk byla zvolena kostn\u00ED d\u0159e\u0148 ky\u010Deln\u00ED kosti. Zdrojem chondrocyt\u016F byl odebran\u00FD vzorek z nez\u00E1t\u011B\u017Eov\u00E9 \u010D\u00E1sti kloubn\u00ED plochy dist\u00E1ln\u00ED epif\u00FDzy stehenn\u00ED kosti a jejich dal\u0161\u00ED kultivace v \u017Eivn\u00E9m mediu. Nov\u011B pou\u017Eit\u00FD skafold byl na b\u00E1zi kolagenu obsahuj\u00EDc\u00EDho nanovl\u00E1kna z chitosanu. Pro zjednodu\u0161en\u00ED manipulace p\u0159i implantaci byly v\u00E1lcovit\u00E9 substr\u00E1ty po lyofilizaci op\u011Bt p\u0159ed\u00E1ny k osazen\u00ED bu\u0148kami v 96-jamkov\u00E9 desti\u010Dce. V\u00E1le\u010Dky byly p\u0159ed implantac\u00ED osazeny 2x106 alogenn\u00EDmi MSC a 1x106 alogenn\u00EDmi CHC. V\u00FDsledky l\u00E9\u010Dby byly hodnoceny na z\u00E1klad\u011B m\u011B\u0159en\u00ED d\u00E9lky kost\u00ED a valg\u00F3zn\u00ED deformace dist\u00E1ln\u00ED epif\u00FDzy stehenn\u00ED kosti a na z\u00E1klad\u011B histologick\u00E9ho vy\u0161et\u0159en\u00ED (charakter a vyzr\u00E1lost novotvo\u0159en\u00E9 tk\u00E1n\u011B, detekce kolagenu II, PAS reakce). Byl sledov\u00E1n rozd\u00EDl mezi nel\u00E9\u010Denou pravou a l\u00E9\u010Denou levou kon\u010Detinou v parametrech r\u016Fst do d\u00E9lky a rozd\u00EDl v \u00FAhlu valgizace. L\u00E9\u010Den\u00E1 lev\u00E1 kon\u010Detina m\u011Bla vy\u0161\u0161\u00ED pr\u016Fm\u011Br r\u016Fstu do d\u00E9lky proti nel\u00E9\u010Den\u00E9 prav\u00E9 kon\u010Detin\u011B (p = 0,004). Pr\u016Fm\u011Brn\u00FD \u00FAhel valgizace dist\u00E1ln\u00ED epif\u00FDzy lev\u00E9 stehenn\u00ED kosti byl men\u0161\u00ED ne\u017E pr\u016Fm\u011Brn\u00FD \u00FAhel valgizace dist\u00E1ln\u00ED epif\u00FDzy prav\u00E9 stehenn\u00ED kosti (p = 0,008). V defektu lev\u00E9 dist\u00E1ln\u00ED stehenn\u00ED kosti byla \u010Dast\u011Bji nalezena tk\u00E1\u0148 bl\u00EDzk\u00E1 hyalin" . "9"^^ . "16170" . . . "Treatment of bone bridge using transplantation of mesenchymal stem cells and chondrocytes in composite scaffold in pigs - experimental study"@en . "RIV/62157124:16170/11:43870446!RIV12-MZ0-16170___" .