. . "Tesa\u0159\u00EDk, R." . "The aim of our study was to extend knowledge about possibilities of replacing challenge tests by in vitro methods in cattle on the model of trichophytosis. We correlated results of three in vitro tests for the detection of immune response, i.e. a specific antigen-driven lymphocyte transformation test measured by (3)H-thymidine incorporation, specific antigen-induced production of interferon-gamma and detection of IgG1 and IgG2 isotypes of specific antibodies, in calves vaccinated against the disease or challenged with Trichophyton verrucosum as causative agent. The results obtained in the present study by different methods are correlated together. Lymphocyte transformation test correlated positively with interferon-gamma production. Ratio of IgG1 to IgG2 isotypes of antibody correlated negatively with both cell-mediated methods. Moreover the results show that any of the methods might in future replace the in vivo challenge tests that are still conventionally used for testing of newly developed vaccine"@en . . "A correlation of in vitro tests for the immune response detection: a bovine trichophytosis model."@en . "46" . . "A correlation of in vitro tests for the immune response detection: a bovine trichophytosis model."@en . "Pavlov\u00E1, B." . . "Krej\u010D\u00ED, E." . "0264-410X" . "The aim of our study was to extend knowledge about possibilities of replacing challenge tests by in vitro methods in cattle on the model of trichophytosis. We correlated results of three in vitro tests for the detection of immune response, i.e. a specific antigen-driven lymphocyte transformation test measured by (3)H-thymidine incorporation, specific antigen-induced production of interferon-gamma and detection of IgG1 and IgG2 isotypes of specific antibodies, in calves vaccinated against the disease or challenged with Trichophyton verrucosum as causative agent. The results obtained in the present study by different methods are correlated together. Lymphocyte transformation test correlated positively with interferon-gamma production. Ratio of IgG1 to IgG2 isotypes of antibody correlated negatively with both cell-mediated methods. Moreover the results show that any of the methods might in future replace the in vivo challenge tests that are still conventionally used for testing of newly developed vaccine" . "Rybnik\u00E1\u0159, A." . "A correlation of in vitro tests for the immune response detection: a bovine trichophytosis model." . "Krej\u010D\u00ED, J." . "1"^^ . "[307A91D61B8D]" . . "Obo\u0159ilov\u00E1, E." . . . . . . . . "7"^^ . . "407861" . "7"^^ . "C\u00EDlem studie bylo roz\u0161\u00ED\u0159it poznatky o mo\u017Enostech nahradit experiment\u00E1ln\u00ED \u010Delen\u017En\u00ED testy laboratorn\u00EDmi in vitro metodami u skotu na modelu trichofyt\u00F3zy. Korelovali jsme v\u00FDsledky t\u0159\u00ED test\u016F detekuj\u00EDc\u00EDch imunitn\u00ED odpov\u011B\u010F - test blastick\u00E9 transformace se specifick\u00FDm antigenem m\u011B\u0159en\u00FD inkorporac\u00ED (3)H-thymidinu, produkci interferonu gama po stimulaci specifick\u00FDm antigenem a kvantifikaci IgG1 a IgG2 izotyp\u016F specifick\u00FDch protil\u00E1tek - u zv\u00ED\u0159at vakcinovan\u00FDch a vystaven\u00FDch experiment\u00E1ln\u00ED infekci. V\u00FDsledky z\u00EDskan\u00E9 v t\u00E9to studii spolu korelovaly. Test blastick\u00E9 transformace lymfocyt\u016F koreloval pozitivn\u011B s produkc\u00ED interferonu gama. Pom\u011Br IgG1 a IgG2 izotyp\u016F protil\u00E1tek koreloval negativn\u011B s ob\u011Bma testy bun\u011B\u010Dn\u00E9 imunity. Nav\u00EDc, v\u00FDsledky uk\u00E1zaly, \u017Ee ka\u017Ed\u00E1 z t\u011Bchto metod m\u016F\u017Ee v budoucnu nahradit in vivo \u010Dalen\u017En\u00ED testy, kter\u00E9 jsou st\u00E1le konven\u010Dn\u011B vyu\u017E\u00EDv\u00E1ny p\u0159i testov\u00E1n\u00ED nov\u011B vyv\u00EDjen\u00FDch vakc\u00EDn."@cs . "P(1B44024), Z(MZE0002716201)" . . . "25" . "16170" . "7948-7954" . "A correlation of in vitro tests for the immune response detection: a bovine trichophytosis model." . "NL - Nizozemsko" . "Korelace laboratorn\u00EDch test\u016F pro detekci imunitn\u00ED odpov\u011Bdi: model trichofyt\u00F3zy skotu"@cs . "RIV/62157124:16170/07:00001557!RIV08-MZE-16170___" . . "Trichophytosis; Cattle; Challenge; Immunity; Vaccine; Interferon-gamma"@en . "Faldyna, Martin" . . "Korelace laboratorn\u00EDch test\u016F pro detekci imunitn\u00ED odpov\u011Bdi: model trichofyt\u00F3zy skotu"@cs . "RIV/62157124:16170/07:00001557" . . "Vaccine" . .