. . "NL - Nizozemsko" . . "circovirus; antibodies; quantitative PCR; viremia"@en . "5"^^ . . . . . . . "Na souboru 400 prase\u010D\u00EDch s\u00E9r byla uskute\u010Dn\u011Bna kvantifikace PCV2 viru metodou Real-time PCR a z\u00E1rove\u0148 uskute\u010Dn\u011Bna detekce PCV2 specifick\u00FDch protil\u00E1tek metodou ELISA. Vzorky poch\u00E1zely z farem s konven\u010Dn\u00EDm chovem prasat a z\u00E1rove\u0148 s v\u00FDskytem cirkovirov\u00E9ho ch\u0159adnut\u00ED selat po odstavu. Sledov\u00E1n\u00ED bylo uskute\u010Dn\u011Bno na selatech poch\u00E1zej\u00EDc\u00EDch ze 7 vrh\u016F selat (ve v\u011Bku 6-25 t\u00FDdn\u016F), kter\u00E1 byla odeb\u00EDr\u00E1na v intervalu 2-3 t\u00FDdn\u016F. IgM protil\u00E1tky byly poprv\u00E9 zaznamen\u00E1ny ve v\u011Bku 8 t\u00FDdn\u016F a dos\u00E1hly maxim\u00E1ln\u00ED \u00FArovn\u011B ve 12 t\u00FDdnech st\u00E1\u0159\u00ED; IgG protil\u00E1tky byly poprv\u00E9 zji\u0161t\u011Bny v 10. t\u00FDdnu a vrcholily v 16. t\u00FDdnu. (pr\u016Fm\u011Brn\u00FD titr 1:3500). Myxim\u00E1ln\u00E1 titr viru byl zaznamen\u00E1n ve v\u011Bku 10 t\u00FDdn\u016F (7x10(7) kopi\u00ED genomu/ml s\u00E9ra), virov\u00E1 infekce perzistovala a\u017E do dosp\u011Bl\u00E9ho v\u011Bku."@cs . "The levels of PCV2 specific antibodies and viremia in pigs"@en . . "The levels of PCV2 specific antibodies and viremia in pigs" . . "Research in Veterinary Science" . . "RIV/62157124:16170/07:00001419" . . "Smola, Ji\u0159\u00ED" . . "Hladiny PCV2 specifick\u00FDch protil\u00E1tek a viremie u prasat"@cs . . "Tak\u00E1\u010Dov\u00E1, Krist\u00EDna" . "Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera)."@en . "430926" . "[269CC1534047]" . . "5"^^ . "Celer, Vladim\u00EDr" . "Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera)." . "Trundov\u00E1, M\u00E1ria" . "Molinkov\u00E1, Dobromila" . "The levels of PCV2 specific antibodies and viremia in pigs" . "5" . "7"^^ . "16170" . . "0034-5288" . . "12" . "Lobov\u00E1, Dana" . "274-278" . "The levels of PCV2 specific antibodies and viremia in pigs"@en . . "P(GA524/06/1640)" . . "Hladiny PCV2 specifick\u00FDch protil\u00E1tek a viremie u prasat"@cs . "Carasov\u00E1, Patricie" . "RIV/62157124:16170/07:00001419!RIV08-GA0-16170___" .