"Z(MSM6215712403)" . "3"^^ . "Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry"@en . "3"^^ . . "The goal of the study was to optimize the method of blood and tissue selenium determination in ruminants and implement it in research and diagnostic practice. A method for selenium determination by hydride generation atomic absorption spectrophotometry (HG-AAS) in full blood, blood plasma and serum, the liver, skeletal muscle tissue, the myocardium, and the kidneys was developed. The measurements were verified by using diverse types of reference materials with declared selenium concentration and by a method of yield measurement of calibration solution addition in mineralized samples. The results corresponded with the stated values at the 95% probability level for all reference materials used. This methodology is acceptable as to both detection limit (0.762 mg.l-1) and error of measurement (4.6-15%) required for authorized use in research as well as clinical and preventive livestock medicine." . . . "Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry" . "Stanoven\u00ED selenu v krvi a tk\u00E1n\u00EDch hydridovou technikou atomov\u00E9 absorp\u010Dn\u00ED spektrofotometrie"@cs . . . . "Stanoven\u00ED selenu v krvi a tk\u00E1n\u00EDch hydridovou technikou atomov\u00E9 absorp\u010Dn\u00ED spektrofotometrie"@cs . . "AAS; liver; muscle; myocardium; microwave mineralization"@en . "Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry" . "CZ - \u010Cesk\u00E1 republika" . "Illek, Josef" . . "Pechov\u00E1, Alena" . "Acta veterinaria Brno" . "16170" . "RIV/62157124:16170/05:00000366!RIV06-MSM-16170___" . "483-490" . . . "Pavlata, Leo\u0161" . "The goal of the study was to optimize the method of blood and tissue selenium determination in ruminants and implement it in research and diagnostic practice. A method for selenium determination by hydride generation atomic absorption spectrophotometry (HG-AAS) in full blood, blood plasma and serum, the liver, skeletal muscle tissue, the myocardium, and the kidneys was developed. The measurements were verified by using diverse types of reference materials with declared selenium concentration and by a method of yield measurement of calibration solution addition in mineralized samples. The results corresponded with the stated values at the 95% probability level for all reference materials used. This methodology is acceptable as to both detection limit (0.762 mg.l-1) and error of measurement (4.6-15%) required for authorized use in research as well as clinical and preventive livestock medicine."@en . "8"^^ . "0001-7213" . "Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry"@en . . . . "4" . . "74" . . . "[CF717F9FED75]" . . "C\u00EDlem pr\u00E1ce byla optimalizace metody na stanoven\u00ED selenu v krvi a tk\u00E1n\u00EDch p\u0159e\u017Ev\u00FDkavc\u016F a praktick\u00E9 zaveden\u00ED vyvinut\u00E9 metody do v\u00FDzkumu a diagnostick\u00E9 praxe. Byla zavedena metoda stanoven\u00ED selenu hydridovou technikou atomov\u00E9 absorp\u010Dn\u00ED spektrofotometrie (HG AAS) v pln\u00E9 krvi, krevn\u00ED plazm\u011B a s\u00E9ru, j\u00E1trech, kostern\u00ED svalovin\u011B, myokardu a ledvin\u00E1ch. Ov\u011B\u0159en\u00ED spr\u00E1vnosti m\u011B\u0159en\u00ED bylo provedeno pou\u017Eit\u00EDm r\u016Fzn\u00FDch typ\u016F referen\u010Dn\u00EDch materi\u00E1l\u016F s deklarovanou koncentrac\u00ED selenu a metodou m\u011B\u0159en\u00ED v\u00FDt\u011B\u017Enosti p\u0159\u00EDdavku kalibra\u010Dn\u00EDch roztok\u016F do mineralizovan\u00FDch vzork\u016F. V\u00FDsledky u v\u0161ech pou\u017Eit\u00FDch referen\u010Dn\u00EDch materi\u00E1l\u016F odpov\u00EDdaly deklarovan\u00FDm hodnot\u00E1m na 95 % hladin\u011B pravd\u011Bpodobnosti. Uveden\u00E1 metodika vyhovuje z hlediska detek\u010Dn\u00EDho limitu (0,762 mg.l-1) i chyby stanoven\u00ED (4,6 - 15 %) po\u017Eadavku pro vyu\u017Eit\u00ED ve v\u00FDzkumu, klinick\u00E9 i preventivn\u00ED medic\u00EDn\u011B hospod\u00E1\u0159sk\u00FDch zv\u00ED\u0159at."@cs . "RIV/62157124:16170/05:00000366" . . "514047" . .