"4"^^ . "CZ - \u010Cesk\u00E1 republika" . "Hauptman, Karel" . "1" . "\u010Cl\u00E1nek popisuje metodu odb\u011Bru krve z vena cava cranialis (VCC) u potkana. S c\u00EDlem omezit stres, odb\u011Br krve by m\u011Bl b\u00FDt proveden pod kr\u00E1tkodobou inhala\u010Dn\u00ED anest\u00E9zi\u00ED, nap\u0159\u00EDklad ve sm\u011Bsi kysl\u00EDk/izolfuran. M\u00EDsto vpichu je t\u011Bsn\u011B p\u0159ed prvn\u00EDm \u017Eebrem, 0,3 -0,8 cm later\u00E1ln\u011B od manubrium sterni p\u0159i dorz\u00E1ln\u00ED poloze zv\u00ED\u0159ete. Jehla, p\u0159ipojen\u00E1 na st\u0159\u00EDka\u010Dku je zavedena pod \u00FAhlem 30\u00B0 sm\u011Brem k femor\u00E1ln\u00ED hlavici opa\u010Dn\u00E9 strany t\u011Bla. Po p\u0159ekon\u00E1n\u00ED k\u016F\u017Ee, je t\u0159eba vyvinout negativn\u00ED tlak ve st\u0159\u00EDka\u010Dce a v zav\u00E1d\u011Bn\u00ED jehly pokra\u010Dovat d\u00E1le ve sm\u011Bru dal\u0161\u00EDch 0,2 a\u017E 1 cm dokud neza\u010Dne t\u00E9ci krev. Je mo\u017En\u00E9 odebrat 0,8 a\u017E 2,5 ml krve v z\u00E1vislosti na t\u011Blesn\u00E9 hmotnosti pacienta. Vyzkou\u0161eli jsme odb\u011Br na 50 potkanech ve v\u011Bku 5 -24 m\u011Bs\u00EDc\u016F, p\u0159i\u010Dem\u017E 25 potkan\u016F bylo odebr\u00E1no jednou, 8 potkan\u016F bylo odbr\u00E1no dvakr\u00E1t v sedmidenn\u00EDm intervalu, 11 potkan\u016F bylo odebr\u00E1no dvakr\u00E1t v intervalu t\u0159\u00ED t\u00FDdn\u016F a \u010Dty\u0159i potkani byly odbr\u00E1ni \u010Dty\u0159ikr\u00E1t v intervalu 4 t\u00FDdn\u016F - celkem 87 odb\u011Br\u016F krve. Z\u00E1va\u017Een\u00E9 komplikace uv\u00E1d\u011Bn\u00E9 p\u0159i odb\u011Bru krve z VCC u jin\u00FDch experim"@cs . "3"^^ . . "RIV/62157124:16170/05:00000250!RIV06-MSM-16170___" . "rodents; small mammals; laboratory diagnostics; bleeding; cranial vena cava; repeated"@en . "514049" . "39" . "Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus)"@en . . . . "This paper describes blood sampling from the cranial vena cava (CVC) in the Norway rat. In order to limit stress, the blood sampling should be done under short-term inhalation anaesthesia, for example, an oxygen/isoflurane mixture. The injection site is just cranial to the first rib, 0.3-0.8 cm lateral to the manubrium when the animal is in dorsal recumbency. The needle, attached to a syringe, is inserted at 30 degrees in the direction of the opposite femoral head. After penetration of the skin, negative pressure is developed in the syringe and the insertion of the needle is continued for another 0.2-1 cm in the given direction until blood begins to flow. The amount of blood sampled ranges from 0.8 to 2.5 mL depending on the body weight of the patient. A trial on 50 rats aged 5-24 months included 25 rats sampled once, eight rats sampled twice with an interval of seven days, 11 rats sampled twice with an interval of three weeks and four rats sampled four times with intervals of four weeks--a total of 8" . . "Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus)" . . . "16170" . . "Knotek, Zden\u011Bk" . "Z(MSM 161700002)" . "This paper describes blood sampling from the cranial vena cava (CVC) in the Norway rat. In order to limit stress, the blood sampling should be done under short-term inhalation anaesthesia, for example, an oxygen/isoflurane mixture. The injection site is just cranial to the first rib, 0.3-0.8 cm lateral to the manubrium when the animal is in dorsal recumbency. The needle, attached to a syringe, is inserted at 30 degrees in the direction of the opposite femoral head. After penetration of the skin, negative pressure is developed in the syringe and the insertion of the needle is continued for another 0.2-1 cm in the given direction until blood begins to flow. The amount of blood sampled ranges from 0.8 to 2.5 mL depending on the body weight of the patient. A trial on 50 rats aged 5-24 months included 25 rats sampled once, eight rats sampled twice with an interval of seven days, 11 rats sampled twice with an interval of three weeks and four rats sampled four times with intervals of four weeks--a total of 8"@en . "236-239" . . "Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus)" . . . . . . . "Jeklov\u00E1, Edita" . "0023-6772" . . "Odb\u011Br krve z vena cava cranialis u potkana (Rattus norvegicus)"@cs . "Laboratory Animals" . . "RIV/62157124:16170/05:00000250" . . "[204744A05309]" . "Odb\u011Br krve z vena cava cranialis u potkana (Rattus norvegicus)"@cs . "4"^^ . "Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus)"@en . . . "Jekl, Vladim\u00EDr" .