"antioxidant capacity; photochem; sample dilution; photochemiluminiscence"@en . "133234" . "This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were infl uenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a nonlinear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a diff erent eff ectiveness of antioxidants and a diff erent stability of their products with radicals." . "2"^^ . . . . . "Bal\u00EDk, Josef" . "8" . "2"^^ . "CZ - \u010Cesk\u00E1 republika" . . . . "Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method"@en . "P(QI91B094)" . "H\u00EDc, Pavel" . . . "6"^^ . . "1211-8516" . "60" . . . "Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method"@en . "[1FECFDCD7F76]" . . . "RIV/62156489:43510/12:00193466!RIV13-MZE-43510___" . "Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method" . "43510" . . . . . "RIV/62156489:43510/12:00193466" . "This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were infl uenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a nonlinear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a diff erent eff ectiveness of antioxidants and a diff erent stability of their products with radicals."@en . "Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method" . "Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis" .