. "[F28AA168C323]" . . "Reliability of phytoplasma ESFY detection by PCR with specific or non-specific primers"@en . "Fytoplazma ESFY v sou\u010Dasn\u00E9 dob\u011B ozna\u010Dovan\u00E9 jako 'Candidatus phytoplasma prunorum' (Seem\u00FCller a Schneider, 2004) se stala hlavn\u00EDm probl\u00E9mem p\u0159i p\u011Bstov\u00E1n\u00ED merun\u011Bk v \u010Cesk\u00E9 republice, a to jak v produk\u010Dn\u00EDch v\u00FDsadb\u00E1ch, tak p\u0159i dop\u011Bstov\u00E1n\u00ED \u0161kolka\u0159sk\u00E9ho materi\u00E1lu. Prvn\u00ED pr\u00E1ce zab\u00FDvaj\u00EDc\u00ED se laboratorn\u00ED diagnostikou fytoplazmy ESFY byly publikov\u00E1ny koncem minul\u00E9ho stolet\u00ED (nap\u0159. Ahrens a Seem\u00FCller, 1992; Lee et al., 1993; Lorenz et al., 1994). V sou\u010Dasn\u00E9 dob\u011B pat\u0159\u00ED mezi nejv\u00EDce pou\u017E\u00EDvan\u00E9 metody pro detekci ESFY polymer\u00E1zov\u00E1 \u0159et\u011Bzov\u00E1 reakce (PCR) v kombinaci s RFLP anal\u00FDzou (Carraro et al., 2001; Jarausch et al., 2000). PCR produkt je mo\u017En\u00E9 tak\u00E9 identifikovat porovn\u00E1n\u00EDm z\u00EDskan\u00FDch sekvenc\u00ED s p\u0159\u00EDslu\u0161n\u00FDmi sekvencemi dan\u00E9ho organizmu v datab\u00E1z\u00EDch (NCBI). V certifika\u010Dn\u00EDm sch\u00E9matu standardu EPPO -- PM 4/30 pro mandlo\u0148, meru\u0148ku, broskvo\u0148 a \u0161vestku je jako dal\u0161\u00ED metoda doporu\u010Deno pou\u017Eit\u00ED d\u0159evit\u00FDch indik\u00E1tor\u016F podno\u017Ee GF305 a odr\u016Fdy meru\u0148ky Luizet) (http://www.eppo.org/index.htm).C\u00EDlem p\u0159edlo\u017Een\u00E9 pr\u00E1ce bylo porovnat di"@cs . . "Kop\u0159ivov\u00E1, Vladim\u00EDra" . "147"^^ . . "396589" . "RIV/62156489:43510/08:00170205" . . . "European stone fruit yellows phytoplasma is called as Candidatus phytoplasma prunorum at present (Seem\u00FCller and Schneider, 2004), is still the main problem in growing apricots in the Czech Republic, both in commercial orchards and in plant nurseries. The first works are engaged in laboratory diagnostic of phytoplasma ESFY, were published in the late last century (for example Ahrens a Seem\u00FCller, 1992; Lee et al., 1993; Lorenz et al., 1994). At the present time one of the most popular methods for detecting ESFY is Polymerase chain reaction in combination with RFLP analysis (Carraro et al., 2001; Jarausch et al., 2000). PCR product is possible identified comparing the resulting sequences with the appropriate sequences for the given organism in databases (for example NCBI). In the EPPO certification scheme standards for almond, apricot, peach and plum, another method recommended is the use of woody indicators (rootstock GF 305 and the apricot variety Luizet). The goal of this work was a comparing diagnost"@en . "Ne\u010Das, Tom\u00E1\u0161" . "Spolehlivost detekce fytoplazmy evropsk\u00E9 \u017Eloutenky peckovin pomoc\u00ED PCR s vyu\u017Eit\u00EDm vybran\u00FDch specifick\u00FDch a nespecifick\u00FDch primer\u016F"@cs . . . "Reliability of phytoplasma ESFY detection by PCR with specific or non-specific primers"@en . . . "S" . . "43510" . "Fytoplazmy v\u00FDznamn\u00E9 patogeny rostlin" . "Spolehlivost detekce fytoplazmy evropsk\u00E9 \u017Eloutenky peckovin pomoc\u00ED PCR s vyu\u017Eit\u00EDm vybran\u00FDch specifick\u00FDch a nespecifick\u00FDch primer\u016F" . "Spolehlivost detekce fytoplazmy evropsk\u00E9 \u017Eloutenky peckovin pomoc\u00ED PCR s vyu\u017Eit\u00EDm vybran\u00FDch specifick\u00FDch a nespecifick\u00FDch primer\u016F" . "RIV/62156489:43510/08:00170205!RIV11-MSM-43510___" . . "Univerzita Palack\u00E9ho v Olomouci a \u010Cesk\u00E1 fytopatologick\u00E1 spole\u010Dnost" . "Spolehlivost detekce fytoplazmy evropsk\u00E9 \u017Eloutenky peckovin pomoc\u00ED PCR s vyu\u017Eit\u00EDm vybran\u00FDch specifick\u00FDch a nespecifick\u00FDch primer\u016F"@cs . . "Kostelec na Han\u00E9" . . "2"^^ . . "molecular detection; primers; phytoplasma esfy; nested pcr"@en . "2"^^ . "5"^^ . "80-903545-2-1" . "Fytoplazma ESFY v sou\u010Dasn\u00E9 dob\u011B ozna\u010Dovan\u00E9 jako 'Candidatus phytoplasma prunorum' (Seem\u00FCller a Schneider, 2004) se stala hlavn\u00EDm probl\u00E9mem p\u0159i p\u011Bstov\u00E1n\u00ED merun\u011Bk v \u010Cesk\u00E9 republice, a to jak v produk\u010Dn\u00EDch v\u00FDsadb\u00E1ch, tak p\u0159i dop\u011Bstov\u00E1n\u00ED \u0161kolka\u0159sk\u00E9ho materi\u00E1lu. Prvn\u00ED pr\u00E1ce zab\u00FDvaj\u00EDc\u00ED se laboratorn\u00ED diagnostikou fytoplazmy ESFY byly publikov\u00E1ny koncem minul\u00E9ho stolet\u00ED (nap\u0159. Ahrens a Seem\u00FCller, 1992; Lee et al., 1993; Lorenz et al., 1994). V sou\u010Dasn\u00E9 dob\u011B pat\u0159\u00ED mezi nejv\u00EDce pou\u017E\u00EDvan\u00E9 metody pro detekci ESFY polymer\u00E1zov\u00E1 \u0159et\u011Bzov\u00E1 reakce (PCR) v kombinaci s RFLP anal\u00FDzou (Carraro et al., 2001; Jarausch et al., 2000). PCR produkt je mo\u017En\u00E9 tak\u00E9 identifikovat porovn\u00E1n\u00EDm z\u00EDskan\u00FDch sekvenc\u00ED s p\u0159\u00EDslu\u0161n\u00FDmi sekvencemi dan\u00E9ho organizmu v datab\u00E1z\u00EDch (NCBI). V certifika\u010Dn\u00EDm sch\u00E9matu standardu EPPO -- PM 4/30 pro mandlo\u0148, meru\u0148ku, broskvo\u0148 a \u0161vestku je jako dal\u0161\u00ED metoda doporu\u010Deno pou\u017Eit\u00ED d\u0159evit\u00FDch indik\u00E1tor\u016F podno\u017Ee GF305 a odr\u016Fdy meru\u0148ky Luizet) (http://www.eppo.org/index.htm).C\u00EDlem p\u0159edlo\u017Een\u00E9 pr\u00E1ce bylo porovnat di" . .