. "2" . . . "Tom\u0161ovsk\u00FD, Michal" . . "pcr; donkioporia expansa; wood; fungi; dna; serpula lacrymans"@en . "Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions"@en . "Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions"@en . "The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR--based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA."@en . "Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis : Acta of Mendel University of agriculture and forestry Brno = Acta Mendelovy zem\u011Bd\u011Blsk\u00E9 a lesnick\u00E9 univerzity v Brn\u011B" . "Hor\u00E1\u010Dek, Petr" . . . "56" . . . "4"^^ . "CZ - \u010Cesk\u00E1 republika" . "3"^^ . "Bobekov\u00E1, Elena" . "RIV/62156489:43410/08:00125099" . "RIV/62156489:43410/08:00125099!RIV09-MSM-43410___" . "43410" . . "Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions" . "Pou\u017Eit\u00ED metod molekul\u00E1rn\u00ED genetiky pro identifikaci d\u0159evn\u00EDch hub v d\u0159ev\u011Bn\u00FDch konstrukc\u00EDch"@cs . "3"^^ . . "[2852E2CF2391]" . "The aim of the paper is to evaluate the utilization of molecular biology methods for detection of wood decaying fungi directly from decomposed wood using a commercial DNA extraction kit developed for soil substrates (PowerSoil DNA isolation kit). The experiment based on dry rot fungus (Serpula lacrymans) detection from inoculated wooden pieces under laboratory conditions was followed by field detection of wood-decaying fungi from wood structures on building constructions. Fungal DNA was identified using the PCR--based methods including species-specific PCR and sequencing of amplified ITS region of ribosomal DNA." . "C\u00EDlem tohoto \u010Dl\u00E1nku je zhodnotit mo\u017En\u00E9 pou\u017Eit\u00ED molekul\u00E1rn\u011B biologick\u00FDch metod pro detekci d\u0159evn\u00EDch hub p\u0159\u00EDmo z rozlo\u017Een\u00E9ho d\u0159eva za pou\u017Eit\u00ED komer\u010Dn\u00EDho kitu pro izolaci DNA vyvinut\u00E9ho pro p\u016Fdn\u00ED substr\u00E1t. Experiment zalo\u017Een\u00FD na identifikaci d\u0159evomorky dom\u00E1c\u00ED (Serpula lacrymans) z nao\u010Dkovan\u00FDch d\u0159ev\u011Bn\u00FDch \u0161pal\u00ED\u010Dk\u016F v laboratorn\u00EDch podm\u00EDnk\u00E1ch byl n\u00E1sledov\u00E1n ter\u00E9nn\u00ED detekc\u00ED d\u0159evn\u00EDch hub p\u0159\u00EDmo ze d\u0159ev\u011Bn\u00FDch konstrukc\u00ED. Houbov\u00E1 DNA byla identifikov\u00E1na za pou\u017Eit\u00ED metod zalo\u017Een\u00FDch na polymer\u00E1zov\u00E9 \u0159et\u011Bzov\u00E9 reakci (PCR) zahrnuj\u00EDc\u00ED druhov\u011B specifickou PCR a sekvenaci namno\u017Een\u00E9 ITS oblasti ribozom\u00E1ln\u00ED DNA. V\u00FDsledky uk\u00E1zaly, \u017Ee pou\u017Eit\u00FD kit (PowerSoil DNA extraction kit), vyvinut\u00FD v\u00FDrobcem na izolaci z p\u016Fdy, je pom\u011Brn\u011B vhodn\u00FD na izolaci DNA i ze d\u0159eva -- v p\u0159\u00EDpad\u011B laboratorn\u00EDho experimentu se poda\u0159ilo detekovat d\u0159evomorku se 100 % \u00FAsp\u011B\u0161nost\u00ED. Na druhou stranu, v p\u0159\u00EDpad\u011B star\u0161\u00EDch vzork\u016F zetlel\u00E9ho d\u0159eva bez viditeln\u00E9ho mycelia byla \u00FAsp\u011B\u0161nost detekce ni\u017E\u0161\u00ED. U jin\u00FDch vzork\u016F z ter\u00E9nu byla pomoc\u00ED sekvenace ITS oblasti detek"@cs . . "Application of molecular genetic methods for identification of wood-decaying fungi in wood constructions" . "Z(MSM6215648902)" . . "Pou\u017Eit\u00ED metod molekul\u00E1rn\u00ED genetiky pro identifikaci d\u0159evn\u00EDch hub v d\u0159ev\u011Bn\u00FDch konstrukc\u00EDch"@cs . . "1211-8516" . . . . . . . "356765" . .