"Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples"@en . "4"^^ . "Vollmann, Johann" . "1"^^ . . . "Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples"@en . "Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples" . . "Plant Breeding" . "43210" . "Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples" . . . "RIV/62156489:43210/12:00188047" . . . "Lo\u0161\u00E1k, Tom\u00E1\u0161" . "131" . "In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30--60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/ kg were combined, and a NIRS calibration model was developed (cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wide range of sucrose concentrations. Thus, identification of high-sucrose soybean introgressions should be possible in appropriate segregating populations." . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "0179-9541" . . . "seed quality; sucrose content; near infrared reflectance spectroscopy; soybean"@en . "Sato, Takashi" . "Zahlner, Viola" . . . "RIV/62156489:43210/12:00188047!RIV13-MSM-43210___" . "Berghofer, Emmerich" . . . "5"^^ . . "S" . "153471" . "In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30--60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/ kg were combined, and a NIRS calibration model was developed (cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wide range of sucrose concentrations. Thus, identification of high-sucrose soybean introgressions should be possible in appropriate segregating populations."@en . "[FFCE4AE3059D]" . . .