. . "In recent years, biotechnology has permitted regulation of the expression of endogenous plant genes to improve agronomically important traits. Genetic modification of crops has benefited from emerging knowledge of new genes, especially genes that exhibit novel functions. Pea seed-borne mosaic virus (PSbMV), a member of the genus Potyvirus, family Potyviridae, is currently one of the most frequent viral pathogen causes yield losses of legumes. Beside aphid vectors, PSbMV is transmitted by infected seed batches. These losses can be easily prevented by growing resistant cultivars. In pea (Pisum sativum), recessive resistance genes to several potyviruses have been mapped genetically to linkage groups II and VI. Resistance to the common strains of PSbMV is conferred by a single recessive gene eIF4E, localized on linkage group VI (sbm-1 locus). Homologues eIF4E(iso) gene was found in close relation to sbm- 2 locus (on linkage group II). Eukaryotic translation initiation factor 4E (eIF4E) and its isoform eIF4E(iso) play a key role during virus infection in plants. The eIF4E(iso) genes from several pea lines were isolated and sequenced. Full genomic fragments of eIF4E(iso) were obtained and protein sequences were deduced. Mutations of eIF4E(iso) between resistant and susceptible genotypes have been identified but are not yet functionally assigned. Selected genotypes are currently tested by L1 (P2) PSbMV pathotype."@en . "43210" . "2011-01-01+01:00"^^ . "https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf" . "RIV/62156489:43210/11:00180419!RIV15-MSM-43210___" . "Molekul\u00E1rn\u00ED charakterizace genu rezistence hrachu k viru semenem p\u0159enosn\u00E9 mozaiky (PSbMV)" . . "[199577771856]" . "Pea seed-borne mosaic virus (PSbMV) pat\u0159\u00ED mezi RNA viry, do \u010Deledi Potyviridae, rodu Potyvirus. Spolu s virem ena\u010Dn\u00ED mozaiky hrachu (Pea enation mosaic virus, PEMV) a virem \u017Elut\u00E9 mozaiky hrachu (Bean yellow mosaic virus, BYMV) tvo\u0159\u00ED trojici hlavn\u00EDch virov\u00FDch patogen\u016F, zp\u016Fsobuj\u00EDc\u00EDch hospod\u00E1\u0159sky v\u00FDznamn\u00E9 \u0161kody na porostech hrachu a n\u011Bkter\u00FDch dal\u0161\u00EDch z\u00E1stupc\u016F luskovin. Virus PSbMV je p\u0159en\u00E1\u0161en semenem, mechanicky a hmyz\u00EDmu vektory (m\u0161icemi). V p\u0159\u00EDpad\u011B hrachu bylo pops\u00E1no n\u011Bkolik lokus\u016F rezistence k virov\u00FDm patogen\u016Fm z rodu Poytvirus, kter\u00E9 jsou d\u011Bd\u011Bny recesivn\u011B a tvo\u0159\u00ED dva shluky nach\u00E1zej\u00EDc\u00ED se ve dvou odli\u0161n\u00FDch vazebn\u00FDch skupin\u00E1ch (chromozomech). Lokusu sbm-1 (LG-VI) odpov\u00EDd\u00E1 eukaryotick\u00FD transala\u010Dn\u00ED inicia\u010Dn\u00ED faktor 4E (eIF4E) a v t\u011Bsn\u00E9 vazb\u011B s lokusem sbm-2 byl nalezen jeho homolog eIF4E(iso). Sekvenac\u00ED byla z\u00EDsk\u00E1na \u00FApln\u00E1 genomov\u00E1 sekvence eIF4E(iso) vybran\u00FDch polo\u017Eek hrachu. Byly identifikov\u00E1ny mutace v eIF4E(iso) vedouc\u00ED k aminokyselinov\u00E9 z\u00E1m\u011Bn\u011B mezi rezistentn\u00EDmi a n\u00E1chyln\u00FDmi genotypy, nepoda\u0159ilo naj\u00EDt takov\u00E9 rozd\u00EDly mezi senzitivn\u00ED a rezistentn\u00ED alelou, kter\u00E9 by se daly jako v p\u0159\u00EDpad\u011B sbm-1 vyu\u017E\u00EDt p\u0159i MAS (Marker assisted selection)."@cs . . . . "3"^^ . . "Kone\u010Dn\u00E1, Eva" . "7"^^ . "2"^^ . "Mendel University in Brno" . . "MendelNet 2011 - Proceedings of International Ph.D. Students Conference" . "Han\u00E1\u010Dek, Pavel" . "Molekul\u00E1rn\u00ED charakterizace genu rezistence hrachu k viru semenem p\u0159enosn\u00E9 mozaiky (PSbMV)"@cs . . "978-80-7375-563-8" . . "Molekul\u00E1rn\u00ED charakterizace genu rezistence hrachu k viru semenem p\u0159enosn\u00E9 mozaiky (PSbMV)" . "eIF4E; resistance; pea; PSbMV; eIF4E(iso)"@en . . . "S" . "Molekul\u00E1rn\u00ED charakterizace genu rezistence hrachu k viru semenem p\u0159enosn\u00E9 mozaiky (PSbMV)"@cs . . "Molecular characterization of Pea seed-borne mosaic virus (PSbMV) resistance gene"@en . "Brno" . . "Brno" . "213519" . "RIV/62156489:43210/11:00180419" . "Sm\u00FDkal, Petr" . . . "Pea seed-borne mosaic virus (PSbMV) pat\u0159\u00ED mezi RNA viry, do \u010Deledi Potyviridae, rodu Potyvirus. Spolu s virem ena\u010Dn\u00ED mozaiky hrachu (Pea enation mosaic virus, PEMV) a virem \u017Elut\u00E9 mozaiky hrachu (Bean yellow mosaic virus, BYMV) tvo\u0159\u00ED trojici hlavn\u00EDch virov\u00FDch patogen\u016F, zp\u016Fsobuj\u00EDc\u00EDch hospod\u00E1\u0159sky v\u00FDznamn\u00E9 \u0161kody na porostech hrachu a n\u011Bkter\u00FDch dal\u0161\u00EDch z\u00E1stupc\u016F luskovin. Virus PSbMV je p\u0159en\u00E1\u0161en semenem, mechanicky a hmyz\u00EDmu vektory (m\u0161icemi). V p\u0159\u00EDpad\u011B hrachu bylo pops\u00E1no n\u011Bkolik lokus\u016F rezistence k virov\u00FDm patogen\u016Fm z rodu Poytvirus, kter\u00E9 jsou d\u011Bd\u011Bny recesivn\u011B a tvo\u0159\u00ED dva shluky nach\u00E1zej\u00EDc\u00ED se ve dvou odli\u0161n\u00FDch vazebn\u00FDch skupin\u00E1ch (chromozomech). Lokusu sbm-1 (LG-VI) odpov\u00EDd\u00E1 eukaryotick\u00FD transala\u010Dn\u00ED inicia\u010Dn\u00ED faktor 4E (eIF4E) a v t\u011Bsn\u00E9 vazb\u011B s lokusem sbm-2 byl nalezen jeho homolog eIF4E(iso). Sekvenac\u00ED byla z\u00EDsk\u00E1na \u00FApln\u00E1 genomov\u00E1 sekvence eIF4E(iso) vybran\u00FDch polo\u017Eek hrachu. Byly identifikov\u00E1ny mutace v eIF4E(iso) vedouc\u00ED k aminokyselinov\u00E9 z\u00E1m\u011Bn\u011B mezi rezistentn\u00EDmi a n\u00E1chyln\u00FDmi genotypy, nepoda\u0159ilo naj\u00EDt takov\u00E9 rozd\u00EDly mezi senzitivn\u00ED a rezistentn\u00ED alelou, kter\u00E9 by se daly jako v p\u0159\u00EDpad\u011B sbm-1 vyu\u017E\u00EDt p\u0159i MAS (Marker assisted selection)." . "Molecular characterization of Pea seed-borne mosaic virus (PSbMV) resistance gene"@en . "341783400078" . . .