"Brno" . "Z\u00EDtka, Ond\u0159ej" . "Brno" . "Optimalization chromatographic isolation of anthocyanins"@en . . "5"^^ . "RIV/62156489:43210/11:00180367" . . "978-80-7375-563-8" . "2011-01-01+01:00"^^ . "Optimalization chromatographic isolation of anthocyanins"@en . "S" . . . "43210" . "Optimalization chromatographic isolation of anthocyanins" . "Anthocyanins are natural colorants. They have been shown to have some beneficial health effect. Red and amber durum wheats contain as major xanthophylls pigments, carotenoids and flavones. Blue and purple wheats are different from those that contain another group of pigments, anthocyanins. Grain from six wheat lines differing in pigmentation was used in this study: %22UC 660 49%22 (blue aleurone), A. Arraseita (purple perikarp), %22TBS%22 (blue aleurone), %22ANK 28B%22 (purple perikarp), %22N 67%22 (red pericarp), Heroldo (white pericarp). Wheat grains were harvested every five days begining 15th day post anthesis, ending once the grains reached physiological maturity (40th day post anthesis), for a total six sampling dates. Anthocyanins were extracted with 10 mL acidifield methanol (methanol and 1.0 N HCl, 85:15 v/v) and shaking on Ika Ultra-Turrax Tube Drive, for 2x29 min. The filtred extract were evaporated at 50 \u00B0C to dryness and redissolved in acidifield methanol. Individual anthocyanins were separated and and quantifield by highperformance liquid chomatography (HPLC). Anthocyanin concentration increased rapidly during grain development and then decreased before maturity. Cyanidin 3-glucoside was the predominant anthocyanin in purple wheat. Delphinidin 3-glucoside is the principal one in blue wheat. Anthocyanins are considered to be physiologically active substances, whose importance in promoting health and reducing the risk of chronic disease has been scientifically documented. This means that the presence of wheat anthocyanins in pigmented wheat, can be a source of bioactive compounds for use in the food industry."@en . . "Optimalization chromatographic isolation of anthocyanins" . "H\u00FAska, Dalibor" . "Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B" . "Kizek, Ren\u00E9" . . "Chabinov\u00E1, Jana" . . . . . . . "Anthocyanins are natural colorants. They have been shown to have some beneficial health effect. Red and amber durum wheats contain as major xanthophylls pigments, carotenoids and flavones. Blue and purple wheats are different from those that contain another group of pigments, anthocyanins. Grain from six wheat lines differing in pigmentation was used in this study: %22UC 660 49%22 (blue aleurone), A. Arraseita (purple perikarp), %22TBS%22 (blue aleurone), %22ANK 28B%22 (purple perikarp), %22N 67%22 (red pericarp), Heroldo (white pericarp). Wheat grains were harvested every five days begining 15th day post anthesis, ending once the grains reached physiological maturity (40th day post anthesis), for a total six sampling dates. Anthocyanins were extracted with 10 mL acidifield methanol (methanol and 1.0 N HCl, 85:15 v/v) and shaking on Ika Ultra-Turrax Tube Drive, for 2x29 min. The filtred extract were evaporated at 50 \u00B0C to dryness and redissolved in acidifield methanol. Individual anthocyanins were separated and and quantifield by highperformance liquid chomatography (HPLC). Anthocyanin concentration increased rapidly during grain development and then decreased before maturity. Cyanidin 3-glucoside was the predominant anthocyanin in purple wheat. Delphinidin 3-glucoside is the principal one in blue wheat. Anthocyanins are considered to be physiologically active substances, whose importance in promoting health and reducing the risk of chronic disease has been scientifically documented. This means that the presence of wheat anthocyanins in pigmented wheat, can be a source of bioactive compounds for use in the food industry." . . "8"^^ . . . . "Klejdus, Bo\u0159ivoj" . . . "RIV/62156489:43210/11:00180367!RIV12-MSM-43210___" . "5"^^ . . "218690" . "MendelNet 2011" . . "[FA43461C251F]" . "optimalization; chromatographic isolation; anthocyan"@en .