"A novel method for analysis of plants' resistance to heavy metals"@en . "A novel method for analysis of plants' resistance to heavy metals"@en . . . "Z\u00EDtka, Ond\u0159ej" . . "\u0160obrov\u00E1, Pavl\u00EDna" . "7"^^ . . "7"^^ . "Zehn\u00E1lek, Josef" . "43210" . . "Beklov\u00E1, Miroslava" . "Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protective mechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable tool for determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2." . "Brno" . . "MendelNet 2011" . "Brno" . "Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protective mechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable tool for determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2."@en . . "978-80-7375-563-8" . "5"^^ . . . . "RIV/62156489:43210/11:00180218!RIV15-MSM-43210___" . "[C1C6F64FBB0D]" . "S" . "341783400130" . . "Kry\u0161tofov\u00E1, Olga" . "phytochelatin synthese; coulometric detection; heavy metals"@en . . "Adam, Vojt\u011Bch" . "Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B" . "Kizek, Ren\u00E9" . "https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf" . . "184021" . . "A novel method for analysis of plants' resistance to heavy metals" . . . . "RIV/62156489:43210/11:00180218" . "A novel method for analysis of plants' resistance to heavy metals" . . "2011-01-01+01:00"^^ . . . .