"978-80-86793-17-7" . "RIV/62156489:43210/11:00174345!RIV12-GA0-43210___" . . . "Masarykuv Onkologicky ustav v Brne" . . . . "218613" . . . . "Optimization, detection, and analysis of DNA intercalation"@en . . . "[88ADF5DD5302]" . "Hub\u00E1lek, Jarom\u00EDr" . "Adam, Vojt\u011Bch" . "Eckschlager, Tom\u00E1\u0161" . "Pro sledov\u00E1n\u00ED interakce protin\u00E1dorov\u00E9ho l\u00E9\u010Diva doxorubicinu s DNA jsme vyu\u017Eili elektrochemickou techniku cyklick\u00E9 volumetrie. Byla m\u011B\u0159ena DNA izolovan\u00E1 z bun\u011Bk senzitivn\u00EDch a rezistentn\u00EDch k doxorubicinu. Z\u00EDskali jsme charakteristick\u00FD voltamogram DNA izolovan\u00E9 z bun\u011Bk. Na pr\u016Fb\u011Bhu z\u00E1znamu jsme pozorovali dva p\u00EDky. Prvn\u00ED p\u00EDk (1) odpov\u00EDd\u00E1 reduk\u010Dn\u00EDmu sign\u00E1lu cytosinu a adeninu (kolem potenci\u00E1lu -1.5 V), druh\u00FD p\u00EDk (2) odpov\u00EDd\u00E1 oxida\u010Dn\u00EDmu sign\u00E1lu guaninu (kolem potenci\u00E1lu -0.2 V). D\u00E1le jsme studovali vliv 0,1 uM a 1 uM koncentrace doxorubicinu na sign\u00E1l p\u0159i rychlosti polarizace 1280 mV/s. DNA izolovan\u00E1 jak z rezistentn\u00EDch tak i senzitivn\u00EDch bun\u011Bk byla tedy m\u011B\u0159ena p\u0159i r\u016Fzn\u00FDch rychlostech polarizace. Z\u00EDskali jsme line\u00E1rn\u00ED z\u00E1vislosti, ze kter\u00FDch jsme ur\u010Dili sm\u011Brnice p\u0159\u00EDmek, kter\u00E9 jsme porovn\u00E1vali se sm\u011Brnicemi kontroln\u00EDho vzorku (bu\u0148ky kultivovan\u00E9 bez p\u0159\u00EDtomnosti doxorubicinu). Obr. 1 a 2 ukazuje pr\u016Fm\u011Brn\u00E9 hodnoty sm\u011Brnic v z\u00E1vislosti na koncentraci doxorubicinu pro resistentn\u00ED, respektive senzitivn\u00ED bu\u0148ky. Na svisl\u00E9 ose jsou vyneseny hodnoty sm\u011Brnic (k), jednotliv\u00E9 sloupce odpov\u00EDdaj\u00ED dan\u00FDm koncentrac\u00EDm. Prvn\u00ED sloupec odpov\u00EDd\u00E1 kontroln\u00EDmu vzorku (bez doxorubicinu). Na Obr. 1 m\u016F\u017Eeme pozorovat statistick\u00FD rozd\u00EDl mezi vzorkem s koncentrac\u00ED doxorubicinu 0,05 a kontroln\u00EDm vzorkem. Pro ostatn\u00ED koncentrace nen\u00ED rozd\u00EDl sm\u011Brnic statisticky v\u00FDznamn\u00FD. T\u00EDm se potvrdila rezistence bun\u011Bk, proto\u017Ee zm\u011Bna hodnoty pr\u016Fm\u011Brn\u00FDch sm\u011Brnic se nem\u011Bn\u00ED v z\u00E1vislosti na koncentraci doxorubicinu."@cs . . "XXXV. Brn\u011Bnsk\u00E9 onkologick\u00E9 dny" . . . . "Optimalizace, detekce, interkalace a analyza DNA"@cs . "Kynclov\u00E1, Hana" . . "For monitoring the interaction of anticancer drug doxorubicin with DNA, we used the electrochemical technique of cyclic volumetry. Was measured by DNA isolated from cells sensitive and resistant to doxorubicin. We obtained voltammograms characteristic of DNA isolated from the cells. On the record, we observed two peaks. The first peak (1) is reductionist cytosine and adenine signal (potential around -1.5 V), the second peak (2) corresponds to the guanine oxidation signal (potential around -0.2 V). We also studied the effect of 0.1 uM and 1 uM concentrations of doxorubicin to the signal polarization at the speed of 1280 mV / s. DNA isolated from both resistant and sensitive cells was then measured at different speeds polarization. We obtained a linear dependence, from which we determined the lines of which we compared with the directives of the control sample (cells cultured without the presence of doxorubicin). Fig. 1 and 2 shows the average value of directives based on the concentration of doxorubicin-resistant, or sensitive cells. The vertical axis values are plotted directives (k), the columns correspond to the given concentrations. The first column corresponds to a control sample (without doxorubicin). In Fig. We can observe a statistical difference between the sample with the concentration of doxorubicin 0.05 and the control sample. For others, the concentration difference is not statistically significant directives. This confirmed the resistance of cells, because the average change in value of guidelines does not change depending on the concentration of doxorubicin."@en . "43210" . . "RIV/62156489:43210/11:00174345" . . "3"^^ . . "Optimalizace, detekce, interkalace a analyza DNA" . "5"^^ . . . "2011-01-01+01:00"^^ . "6"^^ . . "Optimization, detection, and analysis of DNA intercalation"@en . "Optimalizace, detekce, interkalace a analyza DNA"@cs . . "Brno" . "Pro sledov\u00E1n\u00ED interakce protin\u00E1dorov\u00E9ho l\u00E9\u010Diva doxorubicinu s DNA jsme vyu\u017Eili elektrochemickou techniku cyklick\u00E9 volumetrie. Byla m\u011B\u0159ena DNA izolovan\u00E1 z bun\u011Bk senzitivn\u00EDch a rezistentn\u00EDch k doxorubicinu. Z\u00EDskali jsme charakteristick\u00FD voltamogram DNA izolovan\u00E9 z bun\u011Bk. Na pr\u016Fb\u011Bhu z\u00E1znamu jsme pozorovali dva p\u00EDky. Prvn\u00ED p\u00EDk (1) odpov\u00EDd\u00E1 reduk\u010Dn\u00EDmu sign\u00E1lu cytosinu a adeninu (kolem potenci\u00E1lu -1.5 V), druh\u00FD p\u00EDk (2) odpov\u00EDd\u00E1 oxida\u010Dn\u00EDmu sign\u00E1lu guaninu (kolem potenci\u00E1lu -0.2 V). D\u00E1le jsme studovali vliv 0,1 uM a 1 uM koncentrace doxorubicinu na sign\u00E1l p\u0159i rychlosti polarizace 1280 mV/s. DNA izolovan\u00E1 jak z rezistentn\u00EDch tak i senzitivn\u00EDch bun\u011Bk byla tedy m\u011B\u0159ena p\u0159i r\u016Fzn\u00FDch rychlostech polarizace. Z\u00EDskali jsme line\u00E1rn\u00ED z\u00E1vislosti, ze kter\u00FDch jsme ur\u010Dili sm\u011Brnice p\u0159\u00EDmek, kter\u00E9 jsme porovn\u00E1vali se sm\u011Brnicemi kontroln\u00EDho vzorku (bu\u0148ky kultivovan\u00E9 bez p\u0159\u00EDtomnosti doxorubicinu). Obr. 1 a 2 ukazuje pr\u016Fm\u011Brn\u00E9 hodnoty sm\u011Brnic v z\u00E1vislosti na koncentraci doxorubicinu pro resistentn\u00ED, respektive senzitivn\u00ED bu\u0148ky. Na svisl\u00E9 ose jsou vyneseny hodnoty sm\u011Brnic (k), jednotliv\u00E9 sloupce odpov\u00EDdaj\u00ED dan\u00FDm koncentrac\u00EDm. Prvn\u00ED sloupec odpov\u00EDd\u00E1 kontroln\u00EDmu vzorku (bez doxorubicinu). Na Obr. 1 m\u016F\u017Eeme pozorovat statistick\u00FD rozd\u00EDl mezi vzorkem s koncentrac\u00ED doxorubicinu 0,05 a kontroln\u00EDm vzorkem. Pro ostatn\u00ED koncentrace nen\u00ED rozd\u00EDl sm\u011Brnic statisticky v\u00FDznamn\u00FD. T\u00EDm se potvrdila rezistence bun\u011Bk, proto\u017Ee zm\u011Bna hodnoty pr\u016Fm\u011Brn\u00FDch sm\u011Brnic se nem\u011Bn\u00ED v z\u00E1vislosti na koncentraci doxorubicinu." . "Brno" . "H\u00FAska, Dalibor" . "P(GA102/08/1546), P(GAP301/10/0356)" . "detection; optimalization; intercalation"@en . "Optimalizace, detekce, interkalace a analyza DNA" . "Kizek, Ren\u00E9" .