. "4"^^ . "Nesvadbov\u00E1, Michaela" . "RIV/62156489:43210/11:00166608" . . "43210" . . . "The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR"@en . "2"^^ . "CZ - \u010Cesk\u00E1 republika" . . "The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR" . "The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR"@en . . "P(GA523/09/0844)" . "2"^^ . "The evaluation of reference genes for gene expression studies in pig muscle tissue with real-time PCR" . "[CF11B43FD9BE]" . "The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18 has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets."@en . . . "56" . . "Czech Journal of Animal Science" . "5" . "The selection of reference genes is essential for gene expression studies when using real-time quantitative PCR. Reference gene selection should be performed for each individual experiment because the gene expression level may change in different experimental conditions. In this study, the stability of mRNA expression was determined for seven genes: HPRT1, RPS18, NACA, TBP, TAF4B, RPL32 and OAZ1. The stability of these reference genes was investigated in the skeletal muscle tissue of pig foetuses, piglets and adult pigs using real-time quantitative PCR and SYBR green chemistry. The expression stability of used reference genes was calculated using geNorm application. Different gene expression profiles between the age categories of pigs were found. RPS18 has been identified as the gene with the most stable expression in muscle tissue of all pig age categories. HPRT1 and RPL32 were found to have the highest stability in piglets and adult pigs, and in foetuses and adults pigs, respectively. The newly used reference gene, TAF4B, reached the highest expression stability in piglets." . . "198346" . . . "Knoll, Ale\u0161" . . . "1212-1819" . . "RIV/62156489:43210/11:00166608!RIV12-GA0-43210___" . . "reference gene; pig"@en .