"Hub\u00E1lek, Jarom\u00EDr" . "RIV/62156489:43210/10:00166433" . . . "255657" . "Kizek, Ren\u00E9" . . "voltammetry; microparticles; nucleic acid"@en . "8" . "Adam, Vojt\u011Bch" . . . . . "1613-4982" . . "RIV/62156489:43210/10:00166433!RIV11-GA0-43210___" . "4"^^ . . "H\u00FAska, Dalibor" . . "A method that is easy to use, rapid, with a low cost of detecting viral nucleic acid in a biological sample represents the essential tool in targeted therapy. In this study, we report the use of paramagnetic microparticles covered by streptavidin and modified by an oligonucleotide probe with a specific viral sequence labeled by biotin to detect human immunodeficiency virus (HIV) and influenza virus subtype H5N1. The viral nucleic acids were primarily detected by adsorptive transfer stripping technique coupled with square wave voltammetry using carbon paste, hanging mercury drop or carbon nanotubes-based screen-printed working electrodes. Detection limits were estimated for both sequences down to picograms per 3 ul. To isolate the viral sequences, paramagnetic microparticles covered with biotin-labeled oligonucleotides were used. We calculated the yield of isolation for H5N1 and/or HIV sequences, which was defined as %22isolated concentration of viral nucleic acid sequence%22/%22given viral nucleic acid sequ"@en . . "4"^^ . "[BDE36F22B106]" . . . "Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes"@en . "11"^^ . . . . "P(GA102/08/1546), P(KAN208130801)" . "Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes" . . "Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes" . "43210" . "A method that is easy to use, rapid, with a low cost of detecting viral nucleic acid in a biological sample represents the essential tool in targeted therapy. In this study, we report the use of paramagnetic microparticles covered by streptavidin and modified by an oligonucleotide probe with a specific viral sequence labeled by biotin to detect human immunodeficiency virus (HIV) and influenza virus subtype H5N1. The viral nucleic acids were primarily detected by adsorptive transfer stripping technique coupled with square wave voltammetry using carbon paste, hanging mercury drop or carbon nanotubes-based screen-printed working electrodes. Detection limits were estimated for both sequences down to picograms per 3 ul. To isolate the viral sequences, paramagnetic microparticles covered with biotin-labeled oligonucleotides were used. We calculated the yield of isolation for H5N1 and/or HIV sequences, which was defined as %22isolated concentration of viral nucleic acid sequence%22/%22given viral nucleic acid sequ" . "Easy to use and rapid isolation and detection of a viral nucleic acid by using of paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes"@en . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "3" . "Microfluidics and Nanofluidics" . . .