. "Havel, Ladislav" . "RIV/62156489:43210/10:00164691!RIV15-MSM-43210___" . . . . "Pr\u00E1ce je zam\u011B\u0159ena na metodiku izolace celkov\u00E9 RNA ze zaj\u00EDc\u00EDch obilek p\u0161enice s nestandardn\u00ED zabarven\u00EDm obilky jako prvn\u00ED krok pro studium gen\u016F z biosyntetick\u00E9 dr\u00E1hy anthokyan\u016F. Byly odzkou\u0161eny 3 komer\u010Dn\u011B dod\u00E1van\u00E9 kity a na z\u00E1klad\u011B m\u011B\u0159en\u00ED koncetrace a \u010Distoty RNA byl pro dal\u0161\u00ED pr\u00E1ci doporu\u010Den a vyu\u017E\u00EDv\u00E1n kit f. MO BIO. Tento kit poskytuje celkovou RNA v dostate\u010Dn\u00E9m mno\u017Estv\u00ED a \u010Distot\u011B, kter\u00E1 je nutn\u00E1 pro p\u0159epis do cDNA." . "Trojan, V\u00E1clav" . "Pr\u00E1ce je zam\u011B\u0159ena na metodiku izolace celkov\u00E9 RNA ze zaj\u00EDc\u00EDch obilek p\u0161enice s nestandardn\u00ED zabarven\u00EDm obilky jako prvn\u00ED krok pro studium gen\u016F z biosyntetick\u00E9 dr\u00E1hy anthokyan\u016F. Byly odzkou\u0161eny 3 komer\u010Dn\u011B dod\u00E1van\u00E9 kity a na z\u00E1klad\u011B m\u011B\u0159en\u00ED koncetrace a \u010Distoty RNA byl pro dal\u0161\u00ED pr\u00E1ci doporu\u010Den a vyu\u017E\u00EDv\u00E1n kit f. MO BIO. Tento kit poskytuje celkovou RNA v dostate\u010Dn\u00E9m mno\u017Estv\u00ED a \u010Distot\u011B, kter\u00E1 je nutn\u00E1 pro p\u0159epis do cDNA."@cs . "265101" . . . "RIV/62156489:43210/10:00164691" . "Izolace RNA z genetick\u00FDch zdroj\u016F p\u0161enice s nestandardn\u00EDm zabarven\u00EDm obilek" . . "[A3A8B813982E]" . "Musilov\u00E1, Milena" . "Izolace RNA z genetick\u00FDch zdroj\u016F p\u0161enice s nestandardn\u00EDm zabarven\u00EDm obilek"@cs . "978-80-7375-453-2" . . . "RNA isolation; wheat; colored grain"@en . "43210" . . "2010-11-24+01:00"^^ . "Izolace RNA z genetick\u00FDch zdroj\u016F p\u0161enice s nestandardn\u00EDm zabarven\u00EDm obilek"@cs . "341786800108" . . "https://mnet.mendelu.cz/mendelnet2010/mendelnet_2010_fulltext.pdf" . . "4"^^ . "Vyhn\u00E1nek, Tom\u00E1\u0161" . . "Brno" . "Izolace RNA z genetick\u00FDch zdroj\u016F p\u0161enice s nestandardn\u00EDm zabarven\u00EDm obilek" . "Brno" . "Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B" . "MendelNet 2010 - Proceedings of International Ph.D. Students Conference" . . "The RNA isolation from genetic resources of coloured grain wheat"@en . "S" . "5"^^ . . "4"^^ . . "There are several genotypes of wheat with different, genetically determined, grain colour: purple, blue yellow and white. These coloured grain wheat was obtained through wide hybridization among common wheat (Triticum L., 2n=42) and other related genotypes, such as Agropyron elongatum, purple-grained tetraploid wheats from Ethiopia etc. The grown common wheat has red coloured grain. The different coloured grains can be used in food industry for a production of new products which could be not only attractive for consumers but they would be also good for their health. The purple and blue forms content different anthocynanins, compounds well known as antioxidants. Our objective based on flavonoid biochemical pathway was to find genes responsible for different grain coloration in a group of 5 genotypes. The first procedure of the analyses was the RNA isolation, a very important step to manage the whole experiment. The isolation of intact, functional total RNA from coloured-grained wheat containing high levels of starches, polysaccharides, and flavonoids was extremely difficult, that is why were used 3 comercional isolation kits (NucleoSpin RNA Plant - Macherey-Nagel, RNeasy Plant Mini Kit - Qiagen, UltraClean Plant RNA Isolation Sample Kit - MO BIO). There is very important to obtain enough RNA of good quality by the isolation, because there is requested at least 125 ng.ul-1of RNA for RT PCR (Reverse Transcripthase Polymerase Chain Reaction). The range of isolated RNA number is optimal from 125-150 ng.ul-1. According to the results from 3 different isolations 1 kit was chosen. The amount of RNA isolated by the kit UltraClean Plant RNA Isolation Sample Kit (MO BIO) was 530.3 ng.ul-1 of purity 2.1."@en . . "The RNA isolation from genetic resources of coloured grain wheat"@en . .