"Protein based electrochemical biosensor for detection of silver(I) ions"@en . "H\u00FAska, Dalibor" . "Beklov\u00E1, Miroslava" . . . . . "Environmental Toxicology and Chemistry" . . "0730-7268" . . "5"^^ . . . . "RIV/62156489:43210/10:00160473!RIV11-GA0-43210___" . "K\u0159\u00ED\u017Ekov\u00E1, So\u0148a" . "Hub\u00E1lek, Jarom\u00EDr" . "3" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Trnkov\u00E1, Libu\u0161e" . "P(GA102/08/1546), P(GA526/07/0674), P(LC06035)" . "29" . . "Kizek, Ren\u00E9" . "Silver(I) ions are extremely toxic to aquatic animals. Hence, monitoring of these ions in the environment is needed. The aim of the present study was to suggest a simple biosensor for silver(I) ions detection. The suggested biosensor is based on the modification of a hanging mercury drop electrode (HMDE) by the heavy metal binding protein metallothionein (MT) for silver(I) ions detection. Metallothionein accumulated for 120 s onto the HMDE surface. After rinsing the electrode, the biosensor (MT modified HMDE) was prepared prior to detection of silver(I) ions. The biosensor was immersed in a solution containing silver(I) ions. These ions were bound to the MT structure. Furthermore, the electrode was rinsed and transferred to a pure supporting electrolyte solution, in which no interference was present. Under these experimental conditions, other signals relating to heavy metals naturally occurring in MT were not detected. This phenomenon confirms the strong affinity of silver(I) i" . . "Protein based electrochemical biosensor for detection of silver(I) ions" . . . "43210" . . "RIV/62156489:43210/10:00160473" . "Protein based electrochemical biosensor for detection of silver(I) ions" . . "silver(I) ions; voltammetry; biosensor"@en . . "Adam, Vojt\u011Bch" . "283058" . "Silver(I) ions are extremely toxic to aquatic animals. Hence, monitoring of these ions in the environment is needed. The aim of the present study was to suggest a simple biosensor for silver(I) ions detection. The suggested biosensor is based on the modification of a hanging mercury drop electrode (HMDE) by the heavy metal binding protein metallothionein (MT) for silver(I) ions detection. Metallothionein accumulated for 120 s onto the HMDE surface. After rinsing the electrode, the biosensor (MT modified HMDE) was prepared prior to detection of silver(I) ions. The biosensor was immersed in a solution containing silver(I) ions. These ions were bound to the MT structure. Furthermore, the electrode was rinsed and transferred to a pure supporting electrolyte solution, in which no interference was present. Under these experimental conditions, other signals relating to heavy metals naturally occurring in MT were not detected. This phenomenon confirms the strong affinity of silver(I) i"@en . . "7"^^ . . . "7"^^ . "[D7EEE3AFCCBF]" . . "Protein based electrochemical biosensor for detection of silver(I) ions"@en . .