. . "RIV/62156489:43210/10:00155795!RIV10-MSM-43210___" . "Potravin\u00E1rstvo - Food Science (elektronick\u00E1 verze)" . . "Detection of alleles for high-molecular-weight glutenin subunits in triticale using DNA markers"@en . "Detekce alel pro vysokomolekul\u00E1rn\u00ED podjednotky glutenin\u016F u tritikale pomoc\u00ED DNA marker\u016F" . . . "43210" . "RIV/62156489:43210/10:00155795" . "253663" . . . "supplement" . "Vyhn\u00E1nek, Tom\u00E1\u0161" . "Molecular markers were used to identify the allele composition of loci Glu-A1, Glu-B1 and Glu-D1 of high-molecular-weight (HMW) glutenin subunits in 15 triticale genotypes (varieties and breeding lines). Amplified DNA fragments of HMW glutenin alleles were separated by agarose slab-gel electrophoresis. Differences between all the three alleles at the locus Glu-A1 [Glu-A1a (encoding Ax1), 1b (Ax2*) and 1c (AxNull)] were found. We optimised PCR reaction (annealing) of a specific marker for the allele Glu-A1b in PS3 primer combination. In the variety Palomino the alleles Glu-A1b and Glu-A1c were detected from different DNA isolation. DNA was isolated from a mixed sample and so it was not possible to distinguish whether it was an admixture in the seed sample or a heterozygous constitution of the genotype. Two alleles were detected at the locus Glu-B1 [Glu-B1-1a (encoding Bx7) and Glu-B1-1b (Bx7*)] by PS4 primer. The allele Glu-D1d (HMW subunits 5+10) was detected in breeding lines that carried translocati"@en . . . "[EAF0970CE62F]" . "4"^^ . "triticale; markers; Glu-1"@en . . . . "Z(MSM2532885901)" . "Martinek, Petr" . . "4" . "3"^^ . . "Detekce alel pro vysokomolekul\u00E1rn\u00ED podjednotky glutenin\u016F u tritikale pomoc\u00ED DNA marker\u016F"@cs . . "Detekce alel pro vysokomolekul\u00E1rn\u00ED podjednotky glutenin\u016F u tritikale pomoc\u00ED DNA marker\u016F"@cs . . "Trojan, V\u00E1clav" . "SK - Slovensk\u00E1 republika" . "1337-0960" . "Detection of alleles for high-molecular-weight glutenin subunits in triticale using DNA markers"@en . "Molekul\u00E1rn\u00ED markery byly vyu\u017Eity pro identifikaci alelick\u00E9ho slo\u017Een\u00ED v lokusech Glu-A1, Glu-B1 a Glu-D1 pro podjednotky glutenin\u016F s vysokou molekulovou hmotnost\u00ED (VMH) u 15 genotyp\u016F tritikale (odr\u016Fdy a \u0161lechtitelsk\u00E9 linie). Amplifikovan\u00E9 fragmenty pro podjednotky glutenin\u016F s VMH byly separov\u00E1ny na agarosov\u00E9 deskov\u00E9 elektrofor\u00E9ze. Byly nalezeny rozd\u00EDly mezi t\u0159emi alelami v lokusu Glu-A1 [Glu-A1a (k\u00F3duj\u00EDc\u00ED Ax1), Glu-A1b (Ax2*) a Glu-A1c (AxNull)]. Optimalizovali jsme PCR reakci (annealing) s pou\u017Eit\u00EDm primerov\u00E9 kombinace PS3 pro specifick\u00FD marker alely Glu-A1b. U odr\u016Fdy Palomino byly detekov\u00E1ny alely Glu-A1b a Glu-A1c v rozd\u00EDln\u00FDch izolac\u00EDch DNA. Vzhledem k tomu, \u017Ee DNA byla izolov\u00E1na ze sm\u011Bsn\u00FDch vzork\u016F, nen\u00ED mo\u017En\u00E9 rozli\u0161it, zda se jedn\u00E1 o p\u0159\u00EDm\u011Bs ve vzorku osiva nebo o heterozygotn\u00ED konstituci v genotypu. Pomoc\u00ED primeru PS4 byly detekov\u00E1ny dv\u011B alely v lokusu Glu-B1 [Glu-B1-1a (k\u00F3duj\u00EDc\u00ED Bx7) a Glu-B1-1b (Bx7*)]. Alela Glu-D1d (VMH podjednotka 5+10) byla detekov\u00E1na pouze u \u0161lechtitelsk\u00FDch lini\u00ED obsahuj\u00EDc\u00EDc" . "Detekce alel pro vysokomolekul\u00E1rn\u00ED podjednotky glutenin\u016F u tritikale pomoc\u00ED DNA marker\u016F" . "Molekul\u00E1rn\u00ED markery byly vyu\u017Eity pro identifikaci alelick\u00E9ho slo\u017Een\u00ED v lokusech Glu-A1, Glu-B1 a Glu-D1 pro podjednotky glutenin\u016F s vysokou molekulovou hmotnost\u00ED (VMH) u 15 genotyp\u016F tritikale (odr\u016Fdy a \u0161lechtitelsk\u00E9 linie). Amplifikovan\u00E9 fragmenty pro podjednotky glutenin\u016F s VMH byly separov\u00E1ny na agarosov\u00E9 deskov\u00E9 elektrofor\u00E9ze. Byly nalezeny rozd\u00EDly mezi t\u0159emi alelami v lokusu Glu-A1 [Glu-A1a (k\u00F3duj\u00EDc\u00ED Ax1), Glu-A1b (Ax2*) a Glu-A1c (AxNull)]. Optimalizovali jsme PCR reakci (annealing) s pou\u017Eit\u00EDm primerov\u00E9 kombinace PS3 pro specifick\u00FD marker alely Glu-A1b. U odr\u016Fdy Palomino byly detekov\u00E1ny alely Glu-A1b a Glu-A1c v rozd\u00EDln\u00FDch izolac\u00EDch DNA. Vzhledem k tomu, \u017Ee DNA byla izolov\u00E1na ze sm\u011Bsn\u00FDch vzork\u016F, nen\u00ED mo\u017En\u00E9 rozli\u0161it, zda se jedn\u00E1 o p\u0159\u00EDm\u011Bs ve vzorku osiva nebo o heterozygotn\u00ED konstituci v genotypu. Pomoc\u00ED primeru PS4 byly detekov\u00E1ny dv\u011B alely v lokusu Glu-B1 [Glu-B1-1a (k\u00F3duj\u00EDc\u00ED Bx7) a Glu-B1-1b (Bx7*)]. Alela Glu-D1d (VMH podjednotka 5+10) byla detekov\u00E1na pouze u \u0161lechtitelsk\u00FDch lini\u00ED obsahuj\u00EDc\u00EDc"@cs . "Halouzkov\u00E1, Eva" . . "7"^^ .