"RIV/62156489:43210/09:00144736!RIV10-MSM-43210___" . "Martinek, Petr" . "978-80-89417-04-9" . "43210" . "Nov\u00E9 poznatky z genetiky a \u0161\u013Eachtenia po\u013Enohospod\u00E1rskych rastl\u00EDn" . . . "CVRV Pie\u0161\u0165any - V\u00FDskumn\u00FD \u00FAstav rastlinnej v\u00FDroby Pie\u0161\u0165any, Slovensko" . "Pie\u0161\u0165any" . "triticale; Glu-A1; DNA markers"@en . "Identifikace alel lokusu Glu-A1 tritikale pomoc\u00ED DNA marker\u016F"@cs . "Identifikace alel lokusu Glu-A1 tritikale pomoc\u00ED DNA marker\u016F" . . . "Molekul\u00E1rn\u00EDmi markery byly detekov\u00E1ny alely v lokusu Glu-A1 u vybran\u00FDch genotyp\u016F tritikale. Byly detekov\u00E1ny rozd\u00EDly mezi v\u0161emi 3 alelami lokusu Glu-A1 [Glu-A1a (k\u00F3duj\u00EDc\u00ED Ax1), 1b (Ax2*) a 1c (AxNull)]. V p\u0159\u00EDpad\u011B primerov\u00E9 kombinace PS3 byla optimalizov\u00E1na metodika detekce." . "Identifikace alel lokusu Glu-A1 tritikale pomoc\u00ED DNA marker\u016F" . "Identifikace alel lokusu Glu-A1 tritikale pomoc\u00ED DNA marker\u016F"@cs . "Z(MSM2532885901)" . . "[406C25FB2043]" . . "Identification of alleles at the locus Glu-A1 pomoc\u00ED DNA marker\u016F"@en . . "Halouzkov\u00E1, Eva" . "3"^^ . . . "Identification of alleles at the locus Glu-A1 pomoc\u00ED DNA marker\u016F"@en . . . . . "318375" . . "2"^^ . "RIV/62156489:43210/09:00144736" . "Molecular markers were used to identify the allele composition of loci Glu-A1 of high-molecular-weight (HMW) glutenin subunits in fifteen triticale genotypes (varieties and breeding lines). Amplified DNA fragments of HMW glutenin Glu-A1 alleles were separated by agarose slab-gel electrophoresis. Differences between all 3 alleles at the locus Glu-A1 [Glu-A1a (encoding Ax1), 1b (Ax2*) and 1c (AxNull)] were revealed. We optimised of PCR reaction (annealing) of specific marker for the allele Glu-A1b in PS3 primer combination. In the variety Palomino were detected alleles Glu-A1b and Glu-A1c from different DNA isolation. DNA was isolated from a mixed sample and so it was not possible to distinguish if it was detection of admixture in a seed sample or a heterozygous constitution of the genotype."@en . "Vyhn\u00E1nek, Tom\u00E1\u0161" . "2"^^ . . "Molekul\u00E1rn\u00EDmi markery byly detekov\u00E1ny alely v lokusu Glu-A1 u vybran\u00FDch genotyp\u016F tritikale. Byly detekov\u00E1ny rozd\u00EDly mezi v\u0161emi 3 alelami lokusu Glu-A1 [Glu-A1a (k\u00F3duj\u00EDc\u00ED Ax1), 1b (Ax2*) a 1c (AxNull)]. V p\u0159\u00EDpad\u011B primerov\u00E9 kombinace PS3 byla optimalizov\u00E1na metodika detekce."@cs . .