. "C\u00EDlem pr\u00E1ce bylo studium polymorfismu mikrosatelitn\u00EDch marker\u016F na 3H a 7H chromozomu je\u010Dmene u genotyp\u016F je\u010Dmene rezistentn\u00EDch a n\u00E1chyln\u00FDch k Rhynchosporium secalis. Soubor patn\u00E1cti analyzovan\u00FDch genotyp\u016F je\u010Dmene set\u00E9ho (Hordeum vulgare L.) jarn\u00ED formy poch\u00E1z\u00ED z kolekce genetick\u00FDch zdroj\u016F Zem\u011Bd\u011Blsk\u00E9ho v\u00FDzkumn\u00E9ho \u00FAstavu Krom\u011B\u0159\u00ED\u017E, s.r.o.: Abyssinian, Atlas, Atlas 46, Atlas 57, Bay, Cambrinus, Clipper, Jet, Kitchin, Kompakt, Kor\u00E1l, La Mesita, Nigrinudum Abyssinum, Psaknon a Rapid. K anal\u00FDz\u00E1m bylo vybr\u00E1no celkem 33 SSR marker\u016F, z nich\u017E 17 bylo lokalizov\u00E1no na 3H chromozomu je\u010Dmene a 16 SSR marker\u016F bylo lokalizov\u00E1no na 7H chromozomu je\u010Dmene. Mikrosatelitn\u00ED markery byly vybr\u00E1ny z datab\u00E1ze GrainGenes a z literatury, kde je uvedena sekvence primer\u016F, motiv a lokalizace. Celkov\u00E1 genomick\u00E1 DNA byla izolov\u00E1na pomoc\u00ED izola\u010Dn\u00EDho kitu DNeasy Plant Mini Kit od firmy Qiagen (GE) z list\u016F rostlin ve st\u00E1\u0159\u00ED 6--7 dn\u00ED. Koncentrace izolovan\u00E9 DNA byla zji\u0161\u0165ov\u00E1na spektrofotometricky. V\u00FDsledn\u00E9 produkty PCR reakce byly elektrofor"@cs . "RIV/62156489:43210/09:00139259!RIV09-GA0-43210___" . . "Polymorfismus mikrosatelitn\u00EDch marker\u016F na 3H a 7H chromozomu u genotyp\u016F je\u010Dmene rezistentn\u00EDch a n\u00E1chyln\u00FDch k Rhynchosporium secalis"@cs . . "3"^^ . "Vyhn\u00E1nek, Tom\u00E1\u0161" . "Polymorfismus mikrosatelitn\u00EDch marker\u016F na 3H a 7H chromozomu u genotyp\u016F je\u010Dmene rezistentn\u00EDch a n\u00E1chyln\u00FDch k Rhynchosporium secalis"@cs . . "Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis" . "LVII" . "Nevimov\u00E1, Hana" . "334183" . "Bedn\u00E1\u0159, Jan" . . "3"^^ . . "Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis"@en . "The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgare L.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Krom\u011B\u0159\u00ED\u017E, Ltd. showing various degrees of susceptibility to Rhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes against Rhynchosporium secalis is localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one marker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the"@en . "10"^^ . . "Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis : Acta of Mendel University of agriculture and forestry Brno = Acta Mendelovy zem\u011Bd\u011Blsk\u00E9 a lesnick\u00E9 univerzity v Brn\u011B" . . "43210" . . . . . "Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis"@en . "Polymorphism of microsatellite markers on chromosomes 3H and 7H in barley genotypes resistant and susceptible to Rhynchosporium secalis" . . . "RIV/62156489:43210/09:00139259" . . "microsatellite markers; resistance; polymorphism; Rhynchosporium secalis"@en . . "CZ - \u010Cesk\u00E1 republika" . . "The objective of the present study was to explore the polymorphism of microsatellite markers localised on chromosomes 3H and 7H in 15 genotypes of barley (Hordeum vulgare L.), spring form (2n = 2x = 14 chromosomes, genome HVHV) from the collection of genetic resources of the Agricultural Research Institute Krom\u011B\u0159\u00ED\u017E, Ltd. showing various degrees of susceptibility to Rhynchosporium secalis. The selection of SSR markers was based on hitherto achieved knowledge according to which the greatest amount of resistance genes against Rhynchosporium secalis is localised on chromosomes 3H and 7H of barley. We selected 33 SSR markers for the analyses; 17 were localised on chromosome 3H of barley and 16 on chromosome 7H. Out of the total 33 SSR markers, 32 were polymorphous and one marker (Bmac0282) was monomorphic. In total we detected 172 alleles ranging between 101 and 235 bp; the average number of alleles per locus was 5.21. In terms of the polymorphism of the SSR markers localised on chromosomes 3H and 7H the" . "P(GA521/03/0938)" . "1211-8516" . . "2" . "[6CB1888228B2]" .