. "Novel detection of c131y mutation using allele specific PCR (AS \u2013 PCR)"@en . "Stehl\u00EDk, Libor" . "1" . . . "CZ - \u010Cesk\u00E1 republika" . . "RIV/62156489:43210/08:9P000445!RIV09-MZE-43210___" . . "In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattle and the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentioned reasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed using on line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1)." . "1"^^ . . "3"^^ . . "1803-4403" . . "Pomoc\u00ED AS-PCR byla detekov\u00E1na mutace C313Y u plemene gasconne. Mutace je odpov\u011Bdn\u00E1 za dvojite osvaleni u tohoto plemene. D\u016Fv\u011Bryhodnost detekce pomoci nov\u00E9 metody byla ov\u011B\u0159ena sekvenovanim dan\u00E9ho \u00FAseku."@cs . . "Novel detection of c131y mutation using allele specific PCR (AS \u2013 PCR)"@en . "25" . . "In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattle and the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentioned reasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed using on line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1)."@en . "1"^^ . "Novel detection of c131y mutation using allele specific PCR (AS \u2013 PCR)" . "383262" . "Journal of Agrobiology" . "Novel detection of c131y mutation using allele specific PCR (AS \u2013 PCR)" . . "P(1G58073), P(2B08037)" . "Nov\u00FD zp\u016Fsob detekce mutace C313Y pomoc\u00ED alelle specific PCR (AS-PCR)"@cs . . "MSTN; Gasconne; AS-PCR"@en . . . . . "43210" . "RIV/62156489:43210/08:9P000445" . "Nov\u00FD zp\u016Fsob detekce mutace C313Y pomoc\u00ED alelle specific PCR (AS-PCR)"@cs . "[0CCE829B2109]" .