. "6"^^ . . "12th International Conference on Electroanalysis" . . . "metalloproteinases; electrochemistry"@en . "RIV/62156489:43210/08:00134121!RIV09-AV0-43210___" . "Adam, Vojt\u011Bch" . . "43210" . "An Investigation of interactions of matrix metalloproteinases with collagen by chronopotentiometric stripping analysis" . "Matrix metalloproteinases (MMPs) represent a family of structurally related zinc-dependent metallopeptidases. MMPs are able to degrade both majority and minority components of extracellular matrix include the native helix of collagen. These enzymes take part in normal biological processes such as ontogenesis or wound healing but also in inflammatory, generative, and especially malign processes. Zinc ion plays structural and regulatory role in MMPs but it is mainly involved directly in the catalytic hydrolysis of protein substrates. The aim of this work was to propose and optimize the chronopotentiometric stripping analysis (CPSA) in connection with adsorptive transfer technique (AdTS) for MMP-9 detection. The method has been further employed to investigate MMP-9 interaction with collagen. Thanks to AdTS CPSA at hanging mercury drop electrode we were able to measure 5 ul sample volume. We optimized the following parameters: time of the accumulation of the sample on HMDE surface and the composition of" . . "RIV/62156489:43210/08:00134121" . "An Investigation of interactions of matrix metalloproteinases with collagen by chronopotentiometric stripping analysis" . "Trnkov\u00E1, Libu\u0161e" . "Czech Chemical Society" . "[A1B13DBBCBF1]" . "1"^^ . "Z\u00EDtka, Ond\u0159ej" . "H\u00FAska, Dalibor" . . . "Studium interakci mezi matrixov\u00FDmi metaloprotein\u00E1sami s kolagen pomoc\u00ED chronopotenciometrie"@cs . . . "355697" . "Masa\u0159\u00EDk, Michal" . "0009-2770" . "Kizek, Ren\u00E9" . . "Studium interakci mezi matrixov\u00FDmi metaloprotein\u00E1sami s kolagen pomoc\u00ED chronopotenciometrie"@cs . . "P(IAA401990701)" . . "An Investigation of interactions of matrix metalloproteinases with collagen by chronopotentiometric stripping analysis"@en . "Matrix metalloproteinases (MMPs) represent a family of structurally related zinc-dependent metallopeptidases. MMPs are able to degrade both majority and minority components of extracellular matrix include the native helix of collagen. These enzymes take part in normal biological processes such as ontogenesis or wound healing but also in inflammatory, generative, and especially malign processes. Zinc ion plays structural and regulatory role in MMPs but it is mainly involved directly in the catalytic hydrolysis of protein substrates. The aim of this work was to propose and optimize the chronopotentiometric stripping analysis (CPSA) in connection with adsorptive transfer technique (AdTS) for MMP-9 detection. The method has been further employed to investigate MMP-9 interaction with collagen. Thanks to AdTS CPSA at hanging mercury drop electrode we were able to measure 5 ul sample volume. We optimized the following parameters: time of the accumulation of the sample on HMDE surface and the composition of"@en . "Matrix metaloprotein\u00E1z (MMPs) p\u0159edstavuj\u00ED rodinu strukturn\u011B p\u0159\u00EDbuzn\u00FDch zinek-z\u00E1visl\u00E9 metallopeptidasi. MMPs jsou schopny degradovat v\u011Bt\u0161\u00ED \u010Di men\u0161\u00ED slo\u017Eky extracelul\u00E1rn\u00ED matrice obsahuj\u00ED nativn\u00ED helix kolagen. Tyto enzymy se \u00FA\u010Dastn\u00ED v b\u011B\u017En\u00FDch biologick\u00FDch procesech, jako jsou ontogeneze nebo hojen\u00ED ran, ale tak\u00E9 v z\u00E1n\u011Btliv\u00E9, generativn\u00ED, a zejm\u00E9na malign\u00ED procesy. Zine\u010Dnat\u00E9 ionty hraj\u00ED struktur\u00E1ln\u00ED a regula\u010Dn\u00ED \u00FAlohu v MMPs, ale jsou zahrnuty hlavn\u011B p\u0159\u00EDmo v katalytick\u00E9 hydrol\u00FDze bilkov\u00FDnn\u00FDch substr\u00E1t\u016F. C\u00EDlem t\u00E9to pr\u00E1ce bylo navrhnout a optimalizovat chronopotenciometrickou anal\u00FDzu (CPSA) v souvislosti s p\u0159evodem adsorptive technika (AdTS) pro MMP-9 v obraze. Tato metoda byla d\u00E1le vyu\u017E\u00EDv\u00E1na k pro\u0161et\u0159en\u00ED MMP-9 interakce s kolagenem. D\u00EDky AdTS CPSA na vis\u00ED kapka rtuti elektrody jsme byli schopni zm\u011B\u0159it objem 5 ml vzorku. My optimalizov\u00E1ny n\u00E1sleduj\u00EDc\u00ED parametry: doba akumulace vzorku na povrch HMDE a slo\u017Een\u00ED podporuj\u00EDc\u00ED elektrolyt. Vhodn\u00E1 akumula\u010Dn\u00ED doba byla 90 s je pro oba MMP-9 a kolagenu a podporuj\u00ED"@cs . "Prague, Czech Republic" . "6"^^ . . "An Investigation of interactions of matrix metalloproteinases with collagen by chronopotentiometric stripping analysis"@en . . . . . .