"\u0160\u00EDlen\u00FD, Jan" . . "Shestivska, Violetta" . . "43210" . . "Shestivska, Violetta" . "7"^^ . . "Elektrochemick\u00E1 anal\u00FDza interakc\u00ED mez\u00ED DNA a elipticinem pomoc\u00ED minaturizovan\u00FDch elektrod"@cs . . "Electrochemical study of interactions of nucleic acids with ellipticine using miniaturized electrodes" . "1"^^ . "Electrochemical study of interactions of nucleic acids with ellipticine using miniaturized electrodes"@en . "365790" . . . "RIV/62156489:43210/08:00134099!RIV09-AV0-43210___" . . "Adam, Vojt\u011Bch" . "4"^^ . . "Ellipticine (5,11-demethylovan\u00FD-6H-pyridol (4,3-b) karbazolu) je rostlinn\u00FD alcaloid izolov\u00E1n z Apocyanaceae rodiny (nap\u0159. Ochrosia borbonica, Excavativa cocinea). Tato slou\u010Denina a n\u011Bkter\u00E9 jeho deriv\u00E1ty vykazuj\u00ED v\u00FDrazn\u00FD protin\u00E1dorov\u00FD \u00FA\u010Dinnek. Protin\u00E1dorov\u00FD mechanismus ellipticinu spo\u010D\u00EDv\u00E1 v DNA interiakc\u00ED a / nebo inhibice topoizomer\u00E1zy II. Bylo prok\u00E1z\u00E1no, \u017Ee ellipticine aktivov\u00E1n pomoc\u00ED cytochromu P450 je schopen kovalentn\u011B v\u00E1zat na deoxyguanosine ve struktu\u0159e DNA. C\u00EDlem t\u00E9to pr\u00E1ce bylo studium interakc\u00ED ellipticine a DNA pomoc\u00ED miniaturizovan\u00FD elektrody. V na\u0161ich experimentech je vyrobena miniaturizovan\u00FD senzor. Prvn\u00EDm krokem bylo studium ellipticine je (0,5, 1 uM) elektrochemick\u00E9 chov\u00E1n\u00ED pomoc\u00ED cyklick\u00E9 voltametrie a obd\u00E9ln\u00EDkovou vlnou. Z voltammogram\u016F z\u00EDskan\u00E9 oxida\u010Dn\u00ED sign\u00E1ly v potenci\u00E1l asi 600 mV byly pozorovateln\u00E9. D\u00E1le elektrochemick\u00E9 vlastnosti dsDNA byly studov\u00E1ny. Mez detekce dsDNA byla ni\u017E\u0161\u00ED ne\u017E 1 ug / ml a jednotn\u00E9 z\u00E1klady byly odli\u0161eny. V n\u00E1sleduj\u00EDc\u00EDch pokusech interakce ellipticine a"@cs . "Prague, Czech Republic" . . "Electrochemical study of interactions of nucleic acids with ellipticine using miniaturized electrodes" . "RIV/62156489:43210/08:00134099" . "0009-2770" . . "Kizek, Ren\u00E9" . . "H\u00FAska, Dalibor" . "Ellipticine (5,11-demethyl-6H-pyridol(4,3-b)carbazole) is a plant alcaloid isolated from Apocyanaceae family (e.g. Ochrosia borbonica, Excavativa cocinea). This compound and some its derivatives exhibit significant antitumour activity. Mechanism of ellipticine antitumour consists in DNA intercalation and/or topoisomerase II inhibition. It was shown that ellipticine activated by cytochrome P450 is able to bind covalently to deoxyguanosine in DNA structure. The aim of this work was to study of interaction of ellipticine and DNA using miniaturized electrodes. In our experiments the fabricated carbon paste printed electrodes modified with carbon nanotubes were used. The first step was to study of ellipticine's (0.5, 1 uM) electrochemical behavior by using cyclic and square wave voltammetry. In voltammograms obtained the oxidation signals in potential of about 600 mV were observable. Further the electrochemical properties of dsDNA were studied. The detection limit of dsDNA was below 1 ug/ml and single ba" . "Elektrochemick\u00E1 anal\u00FDza interakc\u00ED mez\u00ED DNA a elipticinem pomoc\u00ED minaturizovan\u00FDch elektrod"@cs . "12th International Conference on Electroanalysis" . "ellipticine; DNA"@en . "[D7072B5F67BD]" . "Electrochemical study of interactions of nucleic acids with ellipticine using miniaturized electrodes"@en . . "Czech Chemical Society" . "Hub\u00E1lek, Jarom\u00EDr" . . . "P(KAN208130801)" . "Ellipticine (5,11-demethyl-6H-pyridol(4,3-b)carbazole) is a plant alcaloid isolated from Apocyanaceae family (e.g. Ochrosia borbonica, Excavativa cocinea). This compound and some its derivatives exhibit significant antitumour activity. Mechanism of ellipticine antitumour consists in DNA intercalation and/or topoisomerase II inhibition. It was shown that ellipticine activated by cytochrome P450 is able to bind covalently to deoxyguanosine in DNA structure. The aim of this work was to study of interaction of ellipticine and DNA using miniaturized electrodes. In our experiments the fabricated carbon paste printed electrodes modified with carbon nanotubes were used. The first step was to study of ellipticine's (0.5, 1 uM) electrochemical behavior by using cyclic and square wave voltammetry. In voltammograms obtained the oxidation signals in potential of about 600 mV were observable. Further the electrochemical properties of dsDNA were studied. The detection limit of dsDNA was below 1 ug/ml and single ba"@en . "Stiborov\u00E1, Marie" . .