. "43210" . "Havel, Ladislav" . . "7"^^ . . "1"^^ . "Anal\u00FDza fytochelatin\u016F a fytochelatin syntasy pomoc\u00ED HPLC s elektrochemickou detekc\u00ED"@en . "Diopan, V\u00E1clav" . . "P(1M06030)" . . "7"^^ . "Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection"@cs . . "Adam, Vojt\u011Bch" . "Babula, Petr" . "RIV/62156489:43210/08:00133932" . "7th Workshop on Sulfur in Plants" . . . "Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection" . "Anal\u00FDza fytochelatin\u016F a fytochelatin syntasy pomoc\u00ED HPLC s elektrochemickou detekc\u00ED"@en . "Heavy metals are considered to be dangerous pollutants due to their toxic effects to all organisms. Most of the methods used for remediation of polluted environment suffer from high costs. One of the alternative way represents phytoremediation [1]. This process describes the treatment of environmental problems (bioremediation) through the use of plants. When heavy metals enter into a plant cell, they initiate the biosynthesis of proteins rich in sulphur called phytochelatins, which are used for heavy metal detoxification. Phytochelatins (PC) are cysteine-rich small peptides consist of 4-23 amino acids, which have a basic formula (Glu-Cys)n-Gly (n = 2 to 11). The synthesis of phytochelatins proceeds from glutahione by transferring a Glu-Cys moiety from a donor to an acceptor molecule. The reaction is catalyzed by Glu-Cys dipeptidyl transpeptidase (EC 2.3.2.15), which has been called as phytochelatin synthase (PCS). In vitro the activity of the partially purified enzyme was active only in the presence" . . . "fytochelatins; HPLC; fytochelatinsynthasa"@en . "[38B2D49625C5]" . . "83-917432-8-4" . . . . "H\u00FAska, Dalibor" . . "Institute of Biochemistry and Biophysics, Polish Academy of Sciences" . . . "Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection" . "Warsaw, Poland" . "Baloun, Ji\u0159\u00ED" . "Warsaw, Poland" . "2008-01-01+01:00"^^ . "355860" . "Kizek, Ren\u00E9" . . "RIV/62156489:43210/08:00133932!RIV09-MSM-43210___" . "Analysis of phytochelatin and phytochelatin synthase using liquid chromatography with electrochemical detection"@cs . . . . "T\u011B\u017Ek\u00E9 kovy jsou pova\u017Eov\u00E1ny za nebezpe\u010Dn\u00E9 l\u00E1tky zne\u010Di\u0161\u0165uj\u00EDc\u00ED v\u0161echny organism\u016F. V\u011Bt\u0161ina metod pou\u017E\u00EDvan\u00FDch pro sanace zne\u010Di\u0161t\u011Bn\u00E9ho \u017Eivotn\u00EDho prost\u0159ed\u00ED trp\u00ED vysok\u00FDmi n\u00E1klady. Jedn\u00EDm z alternativn\u00ED zp\u016Fsob p\u0159edstavuje Fytoremediace [1]. Tento proces popisuje l\u00E9\u010Den\u00ED probl\u00E9m\u016F t\u00FDkaj\u00EDc\u00EDch se \u017Eivotn\u00EDho prost\u0159ed\u00ED (Bioremediace) prost\u0159ednictv\u00EDm vyu\u017Eit\u00ED rostlin. Rostliny p\u0159ij\u00EDmaj\u00ED t\u011B\u017Ek\u00E9 kovy a zahajuj\u00ED biosynt\u00E9zu b\u00EDlkovin bohat\u00FDch na SH naz\u00FDvan\u00FDch phytochelatiny, kter\u00E9 se pou\u017E\u00EDvaj\u00ED pro detoxikaci t\u011B\u017Ek\u00FDch kov\u016F. Phytochelatins (PC) jsou bohat\u00E9 na cystein-mal\u00E9 peptidy se skl\u00E1daj\u00ED z 4-23 aminokyselin, kter\u00E9 maj\u00ED z\u00E1kladn\u00ED vzorec (Glu-Cys) n-Gly (n = 2 a\u017E 11). Synt\u00E9za phytochelatins vych\u00E1z\u00ED z kondenzace n\u011Bkolika molekul glutahionu. Tato reakce je catalyzed Glu-Cys dipeptidyl transpeptidase (ES 2.3.2.15), kter\u00E1 byla ozna\u010Dov\u00E1na jako phytochelatin synt\u00E1za (PCS). In vitro aktivita \u010D\u00E1ste\u010Dn\u011B \u010Di\u0161t\u011Bn\u00FD enzym byl aktivn\u00ED pouze v p\u0159\u00EDtomnosti iont\u016F kov\u016F. Nejlep\u0161\u00ED aktiv\u00E1tor PCS byl testov\u00E1n kadmium n\u00E1sleduje st\u0159\u00EDbro, bismut, olovo"@en . "Heavy metals are considered to be dangerous pollutants due to their toxic effects to all organisms. Most of the methods used for remediation of polluted environment suffer from high costs. One of the alternative way represents phytoremediation [1]. This process describes the treatment of environmental problems (bioremediation) through the use of plants. When heavy metals enter into a plant cell, they initiate the biosynthesis of proteins rich in sulphur called phytochelatins, which are used for heavy metal detoxification. Phytochelatins (PC) are cysteine-rich small peptides consist of 4-23 amino acids, which have a basic formula (Glu-Cys)n-Gly (n = 2 to 11). The synthesis of phytochelatins proceeds from glutahione by transferring a Glu-Cys moiety from a donor to an acceptor molecule. The reaction is catalyzed by Glu-Cys dipeptidyl transpeptidase (EC 2.3.2.15), which has been called as phytochelatin synthase (PCS). In vitro the activity of the partially purified enzyme was active only in the presence"@cs . .