. "373206" . . "Investigation of effects of glutathione synthesis inhibition on early somatic embryos treated with cadmium (II) ions" . "RIV/62156489:43210/08:00133467" . . . . . . "Havel, Ladislav" . . "Kizek, Ren\u00E9" . "43210" . "Toxicology Letters" . "V\u00FDzkum efektu inhibice synt\u00E9zi glutathionu v \u010Dasn\u00FDch somatick\u00FDch embri\u00EDch smrku ovlivn\u011Bn\u00FDch kademnat\u00FDmi ionty"@cs . . . . "Baloun, Ji\u0159\u00ED" . . . "glutathione"@en . "Glutathione can be considered as ubiquitous molecule because of its presence in all living organisms even at units of mM. The main aim of this work was to investigate the effect of glutathione biosynthesis inhibitor buthione sulfoximine (BSO) on total content of mRNA and glutathione at early somatic embryos of Spruce treated with various doses of cadmium(II) ions. Primarily we investigated the influence of BSO on the total content of mRNA (transcriptome) at ESEs. We found that the level of total mRNA enhanced for more than 10% at BSO treated ESEs compared to control embryos. At the end of the treatment the highest BSO dose (500 uM) stimulated synthesis of mRNA four times compared to control embryos. Furthermore we aimed our attention on ESEs treated with BSO + cadmium(II) ions. The highest enhancing of transcriptome level was determined at ESEs treated with 500 uM of cadmium(II) ions. Four days after application of the highest dose of BSO + cadmium(II) ions the level of transcriptome increased three t"@en . . . "Beklov\u00E1, Miroslava" . "[CCCAFFD168E4]" . "RIV/62156489:43210/08:00133467!RIV09-GA0-43210___" . . "Kry\u0161tofov\u00E1, Olga" . "P(GA522/07/0692)" . "0378-4274" . . "NL - Nizozemsko" . "1"^^ . . "8"^^ . "Investigation of effects of glutathione synthesis inhibition on early somatic embryos treated with cadmium (II) ions" . "Zehn\u00E1lek, Josef" . "180" . "Glutathion m\u016F\u017Ee b\u00FDt pova\u017Eov\u00E1no za v\u0161udyp\u0159\u00EDtomn\u00E9 molekuly, proto\u017Ee jeho p\u0159\u00EDtomnost ve v\u0161ech \u017Eiv\u00FDch organismech i na jednotk\u00E1ch mm. Hlavn\u00EDm c\u00EDlem t\u00E9to pr\u00E1ce bylo vy\u0161et\u0159it vliv Glutathion inhibitor biosynt\u00E9zy buthione sulfoximine (BSO) na celkov\u00FD obsah mRNA a Glutathion v ran\u00FDch somatick\u00FDch embry\u00ED smrku zach\u00E1zet s r\u016Fzn\u00FDmi d\u00E1vkami kadmia (II) iont\u016F. Hlavn\u00ED jsme zkoumali vliv BSO na celkov\u00FD obsah mRNA (transkriptomu) na environment\u00E1ln\u011B vhodn\u00FD energetick\u00FD syst\u00E9m. Zjistili jsme, \u017Ee \u00FArove\u0148 celkov\u00E9 mRNA pos\u00EDlen\u00E9 o v\u00EDce ne\u017E 10% na BSO zach\u00E1zet environment\u00E1ln\u011B vhodn\u00FD energetick\u00FD syst\u00E9m v porovn\u00E1n\u00ED s kontroln\u00ED embry\u00ED. Na konci l\u00E9\u010Dby nejvy\u0161\u0161\u00ED BSO d\u00E1vka (500 uM) stimulov\u00E1na synt\u00E9za mRNA \u010Dty\u0159n\u00E1sobek v porovn\u00E1n\u00ED s kontroln\u00ED embry\u00ED. D\u00E1le jsme na\u0161i pozornost zam\u011B\u0159ena na environment\u00E1ln\u011B vhodn\u00FD energetick\u00FD syst\u00E9m zach\u00E1z\u00ED s BSO + kadmium (II) iont\u016F. Nejvy\u0161\u0161\u00ED zv\u00FD\u0161en\u00ED o transkriptomu \u00FArove\u0148 byla stanovena na environment\u00E1ln\u011B vhodn\u00FD energetick\u00FD syst\u00E9m zach\u00E1z\u00ED s 500 uM kadmia (II) iont\u016F. \u010Cty\u0159i dny po aplikaci na nejvy\u0161\u0161\u00ED d\u00E1vku"@cs . "Investigation of effects of glutathione synthesis inhibition on early somatic embryos treated with cadmium (II) ions"@en . "H\u00FAska, Dalibor" . . "8"^^ . "Investigation of effects of glutathione synthesis inhibition on early somatic embryos treated with cadmium (II) ions"@en . "1" . "Glutathione can be considered as ubiquitous molecule because of its presence in all living organisms even at units of mM. The main aim of this work was to investigate the effect of glutathione biosynthesis inhibitor buthione sulfoximine (BSO) on total content of mRNA and glutathione at early somatic embryos of Spruce treated with various doses of cadmium(II) ions. Primarily we investigated the influence of BSO on the total content of mRNA (transcriptome) at ESEs. We found that the level of total mRNA enhanced for more than 10% at BSO treated ESEs compared to control embryos. At the end of the treatment the highest BSO dose (500 uM) stimulated synthesis of mRNA four times compared to control embryos. Furthermore we aimed our attention on ESEs treated with BSO + cadmium(II) ions. The highest enhancing of transcriptome level was determined at ESEs treated with 500 uM of cadmium(II) ions. Four days after application of the highest dose of BSO + cadmium(II) ions the level of transcriptome increased three t" . "Adam, Vojt\u011Bch" . "V\u00FDzkum efektu inhibice synt\u00E9zi glutathionu v \u010Dasn\u00FDch somatick\u00FDch embri\u00EDch smrku ovlivn\u011Bn\u00FDch kademnat\u00FDmi ionty"@cs . .