"Adam, Vojt\u011Bch" . . "180" . "Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one of the possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidized GSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. Finally, we utili"@en . "Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions"@en . "Elektroanal\u00FDza komplexu cisplatiny s glutathionem a interakce cisplatiny s DNA"@cs . "Toxicology Letters" . "RIV/62156489:43210/08:00133454" . "Elektroanal\u00FDza komplexu cisplatiny s glutathionem a interakce cisplatiny s DNA"@cs . . . "1" . . "glutathion; DNA; biointeraction"@en . "Kizek, Ren\u00E9" . "365759" . . . . "Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions" . . "Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions"@en . "43210" . "[DEEAD23C5F7D]" . "Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions" . . . "0378-4274" . . . . "5"^^ . . "5"^^ . "P(IAA401990701)" . . "Kuli vysok\u00E9 reaktivit\u011B SH-skupin glutathionu nen\u00ED p\u0159ekvapuj\u00EDc\u00ED, \u017Ee glutathion m\u016F\u017Ee ovliv\u0148ovat l\u00E9\u010Dbu nemoc\u00ED v\u010Detn\u011B onkologick\u00FDch. V pr\u00E1ci jsme se elektrochemick\u00FDmi metodami pokusili charakterizovat GSH a oxidovan\u00FD glutathion s ohledem na dosa\u017Een\u00ED nejni\u017E\u0161\u00EDch detek\u010Dn\u00EDch limit\u016F. Za nejvhodn\u011Bj\u0161\u00EDch podm\u00EDnek jsme dos\u00E1hli limit\u016F GSH/GSSG - 100 pg / ml a 50 ng / ml, resp. Pak byla zkoum\u00E1na interakce GSH a cisplatiny a DNA. Pr\u016Fm\u011Brn\u00E9 mno\u017Estv\u00E9 l\u00E9\u010Debn\u00E9 cisplatiny nav\u00E1zan\u00E9 na 500 ng DNA byla 8,1 ng. Elektrochemick\u00E1 detekce p\u0159edstavuje alternativn\u00ED methodu pro stanoven\u00ED biologicky aktivn\u00EDch slou\u010Denin. Zde jsme uk\u00E1zaly r\u016Fzn\u00E9 elektrochemick\u00E9 techniky jako vhodn\u00E9 n\u00E1stroje pro sledov\u00E1n\u00ED interakce mezi thioly, cytostatik a DNA."@cs . "H\u00FAska, Dalibor" . . "Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one of the possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidized GSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. Finally, we utili" . . . "1"^^ . "Z\u00EDtka, Ond\u0159ej" . "NL - Nizozemsko" . "RIV/62156489:43210/08:00133454!RIV09-AV0-43210___" . . "Trnkov\u00E1, Libu\u0161e" .